Introduction: Over the past decade, there has been lot of reports which prove
the association of Diabetes Mellitus, which can cause potential neuronal impairment
and cognitive deficits. It has been shown that Diabetes can cause dendritic atrophy
in crucial brain structures like hippocampus and prefrontal cortex which are considered
to the seat of memory and decision making. Most of the herbal extracts used for
treatment of diabetes has focused mostly on the hypoglycaemic and anti-oxidant property
and there are no studies which focused on its effect on altered dendritic architecture
caused by Diabetes. This study was taken up to explore the effect of administration
of Trigonella Foneum Graceum (Known as Fenugreek, Methi in Hindi) seed on diabetes
induced dendritic atrophy in the Hippocampus of adult male Wistar rats.
Materials & Methods: Experimental diabetes was induced by administering
single dose of Streptozotocin (60 mg/kg) through intraperitoneal dose. After inducing
diabetes, treatment groups of rats were orally administered Tfg (1 g/kg) for 6 weeks.
Following this, rats were sacrificed and the brains were removed, processed for
the Golgi-Cox stain method, and analyzed by the Sholl’s method.
Results: Clearly, the rats with diabetes mellitus induced by streptozotocin
showed a decrease in the dendritic length and branching points in both apical and
basal dendrites of both CA1 and CA3 pyramidal neurons from all the analyzed segments.
Interestingly, the Fenugreek treated rats showed the reversal of dendritic atrophy
in most of the segments analyzed in both CA1 and CA3 pyramidal neurons.
Conclusion: The present study demonstrates that Fenugreek seed extract having
a proven hypoglycaemic and anti-diabetic property also processes protection to the
Hippocampal pyramidal neurons form Diabetes associated Neuronal atrophy.
seed, Dendritic atrophy, Diabetes Mellitus, Golgi Cox staining.