A Review: Use of Deuterated Internal Standards in Mass Spectometry Techniques
drugs are used to prevent transplant rejections in organ-transplanted patients
but there has to be effective therapeutic drug monitoring because an inadequate
or incorrect dosage could lead to intoxication or transplant rejection. There
have been many designed protocols for the quantification of these drugs and
most of them involve the use of high performance liquid chromatography but
Armin et al in their research work chose to design a protocol following the
mass spectrometry method which is an upcoming method due to its specificity and
sensitivity which was observed to be notably higher. Five immunosuppressive
drugs which included Cyclosporine A, Tacrolimus, Sirolimus, Everolimus and
Mycophenolic acid were evaluated in whole blood and plasma using deuterated
[1.] Athol J., David W.H. (1999). Therapeutic drug
monitoring of immunosuppressant drugs. Br J ClinPharmacol April, 47(4):
[2.] Siuzdak G. (1994). The emergence of mass spectrometry
in biochemical research. ProcNatlAcadSci U.S.A. 22, 91(24): 11290-7.
[3.] Skoog D.A. (1998). Principles of instrumental
analysis: introduction, pp.18. Harcourt Brace.
[4.] Weiling J. (2002). LC-MS-MS experiences with internal
standards. Chromatographia volume 55 issue 1 supplement, pp S107-S113.