A Review: Use of Deuterated Internal Standards in Mass Spectometry Techniques

Download Article

Authors : Opeyemi Orhekafore Bedu


Immunosuppressive drugs are used to prevent transplant rejections in organ-transplanted patients but there has to be effective therapeutic drug monitoring because an inadequate or incorrect dosage could lead to intoxication or transplant rejection. There have been many designed protocols for the quantification of these drugs and most of them involve the use of high performance liquid chromatography but Armin et al in their research work chose to design a protocol following the mass spectrometry method which is an upcoming method due to its specificity and sensitivity which was observed to be notably higher. Five immunosuppressive drugs which included Cyclosporine A, Tacrolimus, Sirolimus, Everolimus and Mycophenolic acid were evaluated in whole blood and plasma using deuterated internal standards.


[1.] Athol J., David W.H. (1999). Therapeutic drug monitoring of immunosuppressant drugs. Br J ClinPharmacol April, 47(4): 339-350.

[2.] Siuzdak G. (1994). The emergence of mass spectrometry in biochemical research. ProcNatlAcadSci U.S.A. 22, 91(24): 11290-7.

[3.] Skoog D.A. (1998). Principles of instrumental analysis: introduction, pp.18. Harcourt Brace.

[4.] Weiling J. (2002). LC-MS-MS experiences with internal standards. Chromatographia volume 55 issue 1 supplement, pp S107-S113