Synergistic Antioxidant and Antimicrobial Effects of Antrocaryon Micraster and Panda Oleosa
Abstract:
Antrocaryon micraster and Panda oleosa are medicinal plants used in ethnomedicine for treating various ailments, owing to their rich phytochemical profiles and reported bioactivities. This study investigated the antioxidant and antimicrobial properties of these plants, individually and in combination, to assess their therapeutic potential. The study aimed to extract bioactive compounds from A. micraster and P. oleosa using cold maceration, screen for phytochemicals, and evaluate their antioxidant and antimicrobial activities. Plant samples (stem bark) were collected from designated locations in Ghana. Cold maceration with methanol and chloroform was employed for extraction. Phytochemical screening and thin-layer chromatography (TLC) were conducted to identify bioactive constituents. Antioxidant activity was assessed using 2,2-Diphenyl-1-picrylhydrazy (DPPH) and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ABTS radical scavenging assays. Antimicrobial activity was tested against six microorganisms: Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Aspergillus niger, and Escherichia coli. Phytochemical analysis revealed the presence of flavonoids, tannins, saponins, steroids, and glycosides. Both extracts exhibited notable antioxidant properties, with radical scavenging activity ranging from 52.79% to 100% (DPPH) and 90.91% to 100% (ABTS). Antimicrobial assays demonstrated inhibitory effects against the tested microorganisms. A. micraster extract had the lowest Minimum Inhibitory Concentration (MIC) (3.125 mg/mL) and highest Minimum Bactericidal Concentration (MBC) (12.5 mg/mL), while the combined extracts exhibited bactericidal and bacteriostatic effects, as well as fungicidal activity against A. Niger. This study confirms the antioxidant and antimicrobial potential of A. micraster and P. oleosa. Their combined use enhances bioactivity, suggesting potential as sources of natural therapeutic agents. Further research is recommended to explore their clinical applications.References:
[1]. Azwanida, N. N., 2015, A review on
the extraction methods used in medicinal plants: Principles, strengths and
limitations. Medicinal & Aromatic Plants, 4(3), 1–6.
[2]. Bhatt, I. D., Rawat, S., &
Badhani, A., 2017, Antioxidant and nutraceutical potential of selected wild
edible fruits of the Indian Himalayan region. Frontiers in Pharmacology,
8, 515. https://doi.org/10.3389/fphar.2017.00515
[3]. Blois, M. S., 1958, Antioxidant
determinations by the use of a stable free radical. Nature, 181(4617),
1199–1200.
[4]. Cowan, M. M., 1999, Plant products
as antimicrobial agents. Clinical Microbiology Reviews, 12(4),
564–582.
[5]. Cushnie, T. P. T., & Lamb, A. J.,
2005, Antimicrobial activity of flavonoids. International Journal of
Antimicrobial Agents, 26(5), 343–356.
[6]. Eloff, J. N., 1998. A sensitive and
quick microplate method to determine the minimal inhibitory concentration of
plant extracts for bacteria. Planta Medica, 64(8), 711–713.
[7]. Essel, E., Ocloo, A., & Fiagbe,
N., 2017, Phytochemical screening and antimicrobial activity of the bark
extracts of Antrocaryon micraster. International Journal of
Pharmacognosy and Phytochemical Research, 9(5), 675–679.
[8]. Ghosal, S., Singh, S. K., &
Kumar, Y., 1990, Alkaloids and glycosides in medicinal plant pharmacology. Phytotherapy
Research, 4(3), 115–120.
[9]. Handa, S. S., Khanuja, S. P. S.,
Longo, G., & Rakesh, D. D., 2008, Extraction technologies for medicinal and
aromatic plants. International Centre for Science and High Technology.
[10]. Kachkoul, R., El Moussaoui, A.,
Laglaoui, A., & Bourais, I., 2021, In vitro antimicrobial and antioxidant
activity of selected medicinal plant extracts. Journal of Applied
Pharmaceutical Science, 11(4), 135–143.
[11]. Lobo, V., Patil, A., Phatak, A.,
& Chandra, N., 2010, Free radicals, antioxidants and functional foods:
Impact on human health. Pharmacognosy Reviews, 4(8), 118–126.
[12]. Muhoya, B. N., Mburu, D. N., &
Mwangi, B. K., 2017, Phytochemical and antimicrobial analysis of Panda
oleosa root extracts. Journal of Medicinal Plants Studies, 5(3),
107–113.
[13]. Prior, R. L., Wu, X., & Schaich,
K., 2005, Standardized methods for the determination of antioxidant capacity
and phenolics in foods and dietary supplements. Journal of Agricultural and
Food Chemistry, 53(10), 4290–4302.
[14]. Rasooli, I., Razzaghi-Abyaneh, M.,
& Shams-Ghahfarokhi, M., 2022, Mechanisms of antifungal actions of selected
plant-derived compounds. Mycoses, 65(1), 3–17.
[15]. Re, R., Pellegrini, N., Proteggente,
A., Pannala, A., Yang, M., & Rice-Evans, C., 1999, Antioxidant activity
applying an improved ABTS radical cation decolorization assay. Free Radical
Biology and Medicine, 26(9–10), 1231–1237.
[16]. Sharifi-Rad, J., Rodrigues, C. F.,
Stojanovic-Radic, Z. Z., & Aleksic, V., 2020, Antioxidant and antimicrobial
properties of bioactive compounds from plants. Frontiers in Microbiology,
11, 588502. https://doi.org/10.3389/fmicb.2020.588502
[17]. Singleton, V. L., & Rossi, J. A.,
1965, Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid
reagents. American Journal of Enology and Viticulture, 16(3),
144–158.
[18]. Sofowora, A., 1993, Medicinal plants
and traditional medicine in Africa. Spectrum Books Ltd.
[19]. Sies, H., 2020, Oxidative stress: A
concept in redox biology and medicine. Redox Biology, 29, 101521.
[20]. Tugume, P., & Nyakoojo, C., 2019,
Ethnobotanical survey of medicinal plant species used by communities around
Mabira Central Forest Reserve, Uganda. Journal of Ethnobiology and
Ethnomedicine, 15(1), 1–21.
[21]. Vidal Bonifácio, B. G., Silva, M.
L., Ferreira, M. A. N. D., & Barbosa, W. L., 2014, Antibacterial activity
of plant extracts against antibiotic-resistant bacteria. Revista Brasileira
de Farmacognosia, 24(1), 9–16.
[22]. Wagner, H., & Bladt, S., 1996,
Plant drug analysis: A thin layer chromatography atlas (2nd ed.). Springer-Verlag.
[23]. Vigbedor, B. Y., Essel, E., &
Klu, Y., 2022, Evaluation of antioxidant properties of some Ghanaian medicinal
plants. African Journal of Biomedical Research, 25(1), 45–54.
[24]. Adwas, A. A., Elsayed, A. S., Azab,
A. E., & Quwaydir, F. A., 2019, Oxidative stress and antioxidant mechanisms
in human body. Journal of Applied Biotechnology & Bioengineering, 6(1),
43–47.
[25]. Gulcin, I., 2020, Antioxidants and antioxidant methods: An updated overview. Archives of Toxicology, 94, 651–715.
