The vascular complication of diabetes
mellitus is a problem for the patient, and the ability to cope with the disease
and the associated inflammation is a critical aspect of diabetes. Cytokines-induced
inflammation in vascular endothelial cells (VECs) plays an active role in chronic
diseases such as atherosclerosis, diabetes mellitus, kidney injury, and stroke.
We investigated the role of total salvianolic acids (TSA), total tanshinones (TTSN),
and their combination (TSA/TTSN) on the activated vascular endothelial cell and
its inhibitory effect on signal transduction and cytokines regulation. In the extracellular
medium of the injury model of human umbilical vein endothelial cells (HUVECs) induced
by thrombin, the human IL-6, VCAM-1, and ICAM-1 were significantly elevated (p ˂
0.05). However, suppression in the TSA, TTSN, and TSA/TTSN (100 µg/L)-treated groups
(p > 0.05) were notable. TSA alone but not TTSN and TSA/TTSN combination, inhibited
the expression of P-selectin (p < 0.05) and E-selectin (p < 0.01) respectively,
in VECs. Western blot analysis showed JAK and STAT expression in VECs however, the
protein expression was modest in the Salvia miltiorrhiza-treated groups, indicating
the potential of TSA/TTSN in the inflammatory pathways of IL-6, JAK, and STAT signal
transduction in endothelial cells (ECs). This study has made novel observations
regarding the components of Salvia miltiorrhiza regulatory effect on cytokines in
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