Abstract:

Medicinal plant products have been used in health care for the treatment of human illnesses since old times. Various homegrown medicinal plants have been used the cure of blood-related issues due to the disturbance of the coagulation pathway. The goal of the study is to characterise the isolated plant leaf extracts of Tridax procumbens that displayed strong anticoagulant properties. The objective of the study is to demonstrate haemolytic activity, wound healing activity, and assessment of the impurity in the partially purified leaf extract from the plant Tridax procumbens. The same fractions were assessed for pro- or anti-angiogenic properties as their therapeutic potential and cellular toxicity for the safety and efficacy of the purified plant extract. The purified leaf extracts contained 31% carbohydrates, 1.1% protein, and 0.9% lipids. The primary active component responsible for the anticoagulant activity is likely to be Azulene, a naphthalene-based derivative. A significant cellular migration was observed, assisting the wound healing. The anti-angiogenic was observed in the purified leaf extracts with no significant cellular toxicity by haemolysis and MTT assay. Traces of protein and lipid sources are present in the purified leaf extract fractions. About 31% of the leaf-extracted fraction has carbohydrates as an active anticoagulant content. The extract showed 90-60% cell migration, suggesting it to be a potent wound healing ability. The purified fractions did not show cytotoxicity at a concentration of 4-500 μg/mL in T47D cells.

References:

[1]. Bhat, R. B., Etejere, E. O., Oladipo, V. T., 1990, Ethnobotanical studies from central Nigeria. Economic Botany, 44(3), 382-390.

[2]. Diwan, P. V., Tilloo, L. D., & Kulkarni, D. R., 1982, Influence of Tridax procumbens on wound healing. Indian J Med Res, 75, 460-464.

[3]. Jachak, S. M., Gautam, R., Selvam, C., Madhan, H., Srivastava, A., & Khan, T., 2011, Anti-inflammatory, cyclooxygenase inhibitory and antioxidant activities of standardized extracts of Tridax procumbens L. Fitoterapia, 82(2), 173-177. doi:10.1016/j.fitote.2010.08.016

[4]. Singh, C. P., Mishra, P. K., & Gupta, S. P., 2016, Design and Formulation of Tridax procumbens based Polyherbal Cream for Wound Healing Potential. Pharm. Lett., 8, 15-21

[5]. Taddei, A., & Rosas-Romero, A. J., 2000, Bioactivity studies of extracts from Tridax procumbens. Phytomedicine, 7(3), 235-238. doi:10.1016/S0944-7113(00)80009-4

[6]. Tiwari, U., Rastogi, B., Singh, P., Saraf, D. K., & Vyas, S. P., 2004, Immunomodulatory effects of aqueous extract of Tridax procumbens in experimental animals. J Ethnopharmacol, 92(1), 113-119. doi:10.1016/j.jep.2004.02.001

[7]. Cushnie, T., & Lamb, A., 2005, Antimicrobial activity of flavonoids International Journal of Antimicrobial Agents 26 (5): 343-56.

[8]. Ndebia, E., Kamgang, R., & Nkeh-ChungagAnye, B., 2007, Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum torvum (Solanaceae). African Journal of Traditional, Complementary and Alternative Medicines, 4(2), 240-244.

[9]. Ramachandran, V., Kumar, G. V., Sugumar, S., Sundaram, V., & Ahamed, H. N., 2020, Cardioprotective effect of Tridax procumbens Linn in Isoproterenol Induced Myocardial Infarction in rats. Research Journal of Pharmacy and Technology, 13(4), 1923-1927.

[10]. Thalkari, A. B., Karwa, P. N., Shinde, P. S., Gawli, C. S., & Chopane, P. S., 2020, Pharmacological actions of Tridax procumbens L.: a scientific review. Research Journal of Pharmacognosy and Phytochemistry, 12(1), 27-30.

[11]. Balkrishna, A., Sharma, N., Srivastava, D., Kukreti, A., Srivastava, S., & Arya, V., 2024, Exploring the Safety, Efficacy, and Bioactivity of Herbal Medicines: Bridging Traditional Wisdom and Modern Science in Healthcare. Future Integrative Medicine, 3(1), 35-49.

[12]. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J., 1951, Protein measurement with the Folin phenol reagent. J Biol Chem, 193(1), 265-275.

[13]. Morse, E. E., 1947, Anthrone in Estimating Low Concentrations of Sucrose. Analytical Chemistry, 19(12), 1012-1013. doi:10.1021/ac60012a021.

[14]. Pasha, A., Kumbhakar, D. V., et al., 2022, Role of Biosynthesized Ag-NPs Using Aspergillus niger (MK503444.1) in Antimicrobial, Anti-Cancer and Anti-Angiogenic Activities. Front. Pharmacol., 12 :812474.doi: 10.3389/fphar.2021.812474

[15]. Rodriguez, L. G., Wu, X., & Guan, J. L., 2005, Wound-healing assay. Methods Mol Biol, 294, 23-29. doi:10.1385/1-59259-860-9:023

[16]. Rajkumar, K., Mvs, S., Koganti, S., & Burgula, S., 2020, Selenium nanoparticles synthesized using Pseudomonas stutzeri (MH191156) show antiproliferative and anti-angiogenic activity against cervical cancer cells. International journal of nanomedicine, 4523-4540.

[17]. Ribatti, D., Vacca, A., Roncali, L., & Dammacco, F., 1996, The chick embryo chorioallantoic membrane as a model for in vivo research on angiogenesis. The International journal of developmental biology, 40(6), 1189-1197

[18]. Kumar, P., Nagarajan, A., & Uchil, P. D., 2018, Analysis of Cell Viability by the MTT Assay. Cold Spring Harb Protoc, 2018(6). doi:10.1101/pdb.prot095505

[19]. Naqash, S. Y., & Nazeer, R. A., 2011, Anticoagulant, antiherpetic and antibacterial activities of sulphated polysaccharide from Indian medicinal plant Tridax procumbens L. (Asteraceae). Appl Biochem Biotechnol, 165(3-4), 902-912. doi:10.1007/s12010-011-9307-y

[20]. Grada, A., Otero-Vinas, M., Prieto-Castrillo, F., Obagi, Z., & Falanga, V., 2017, Research Techniques Made Simple: Analysis of Collective Cell Migration Using the Wound Healing Assay. J Invest Dermatol, 137(2), e11-e16. doi:10.1016/j.jid.2016.11.020

[21]. Ausprunk, D. H., Knighton, D. R., & Folkman, J., 1974, Differentiation of vascular endothelium in the chick chorioallantois: a structural and autoradiographic study. Developmental biology, 38(2), 237-248.

[22]. Manjamalai, A., Kumar, M., & Grace, V., 2012, Essential oil of Tridax procumbens L induces apoptosis and suppresses angiogenesis and lung metastasis of the B16F-10 cell line in C57BL/6 mice. Asian Pacific Journal of Cancer Prevention, 13(11), 5887-5895.

[23]. Burgos-Pino, J., Gual-Orozco, B., Vera-Ku, M., Loria-Cervera, E. N., Guillermo-Cordero, L., Martinez-Vega, P. P., Gamboa-Leon, R., 2023, Acute oral toxicity in BALB/c mice of Tridax procumbens and Allium sativum extracts and (3S)-16,17-didehydrofalcarinol. J Ethnopharmacol, 301, 115840. doi:10.1016/j.jep.2022.115840