The Antimicrobial Properties of Terminalia Glaucescens and Zanthoxylum Zanthoxyloides Extracts and the Spectrum of their Activity

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Authors : Mgbe Chinenye Glory, Mgbe Emeka Kevin

Abstract:

The antimicrobial activity of the ethanol extracts of two Nigerian chewing sticks (Terminalia glaucescens and Zanthoxylum zanthoxyloides) against oral bacteria and fungi isolates implicated in dental caries were assessed. The aim of the study is to isolate microbial organisms associated with dental caries and to assess the antimicrobial activity of the ethanol extracts of Zanthoxylum zanthoxyloides and Terminalia glaucescens stem used as chewing sticks. The dental extracts from 20 (twenty) patients with clinical features of dental caries were collected from School of dental technology and therapy clinic, Trans-ekulu, Enugu. The patients comprised of 8 males and 12 females with ages ranging from 16-40 years. The disc diffusion method was used in evaluating sensitivity to the extracts and it was observed that all the isolates were sensitive to T. glaucescens at different levels of concentration with a MIC and MBC of 6.25mg/ml for S. pyogenes. The isolated and tested dental pathogens were Escherisia coli, Pseudomonas aerugenosa, Streptococcus mutans, Streptococcus pyogenes, Staphylococcus aureus and Candida albicans. However, of the all the isolates E. coli proved resistant to Z. zanthoxyloides extract. Conventional antibiotics were used as positive control while distilled water was used as negative control.

The ability of T. glaucescens and Z. zanthoxyloides extracts to inhibit the growth of the bacteria and fungi in this study is an indication that the plant can be used as a source for antimicrobial agent in the formulation of toothpaste and mouth wash, thus justifying the use of the plant locally as chewing sticks.

Keywords: Dental Caries, Zanthoxylum zanthoxyloides and Terminalia glaucescens.

References:

[1]. Akande, J.A. and Hayashi, Y. (1998). Potency of extract contents from selected tropical chewing sticks against Staphylococcus aureus and Staphylococcus auricularis. World Journal of Microbiology and Biotechnology, 14: 235-238.

[2]. Barry, A.L. and Thornsberry, C.P. (1991). Susceptibility Tests: Diffusion Test Procedures. In: Balows A, editor. Manual of Clinical Microbiology. 5th ed. Boston: Asm International; pp. 1117–25.

[3]. Fejerskov, O. R. and Kidd, E.N. (2003) .Dental caries: the disease and its clinical management. 1st ed. London: Wiley-Blackwell.

[4]. Fudulu, S.O. (1975). Antimicrobial properties of the buffer extracts of chewing sticks used in Nigeria. Planta medica; 27: 123-126.

[5]. Holding J.A. and Colee, J.G.(1971). “Routine biochemical tests,” in Methods in Microbiology, vol. 6, pp. 592–599.

[6]. Holt, J. G., Kreig, N. R., Sneath, P., Staley, J. and Williams, S.(1994).Bergey's Manual of Determinative Bacteriology. 9th Edition.Williams & Wilkins, Baltimore. Pp. 65-73.

[7]. Kleinberg, I. (2002) .“A mixed-bacteria ecological approach to understanding the role of the oral bacteria in dental caries causation: an alternative to Streptococcus mutans and the specific-plaque hypothesis,” Critical Reviews in Oral Biology and Medicine, vol. 13, no. 2, pp. 108–125.

[8]. Ogundiya, M.O., Kolapo, A . L., Okunade, M .B. and Adejumobi, E. (2014). Evaluation of Phytochemical Composition and Antimicrobial Activity of Terminalia glaucescens Against Some Oral Pathogens. Advances Natural & Applied Sciences, 2(2): 89-93.

[9]. Pretorius, J.C., Magama, S. and Zietsman, P.C. (2003).Growth Inhibition of Plant Pathogenic Bacteria and Fungi by Extracts from Selected South African Plant Species. South African. Journal of Botany, 20: 188-192.

[10]. Schaeken, J.M., Van der Hoeven, J.S. and Franken, H.C.(1986). “Comparative recovery of Streptococcus pyogenes on five isolation media, including a new simple selective medium,” Journal of Dental Research, vol. 65, no. 6, pp. 906–908.

[11]. Wolinsky, L.E. and Sote, E.O. (1984). Isolation of natural plaque-inhibiting Substances from Nigerian chewing sticks. Caries Research, 18:216-225.