Exploring the Effects of Lutein on TNFα/NFκB Signaling Molecules Gene Expression in Lung Cancer Cells

Abstract:
Lung cancer remains one of the
leading causes of cancer-related deaths worldwide, necessitating the
exploration of novel therapeutic strategies. In recent years, natural compounds
have gained significant attention for their potential in cancer prevention and
treatment. Lutein, a naturally occurring carotenoid found in various fruits and
vegetables, has demonstrated promising anti-cancer properties. This study aims
to investigate the effects of lutein on the gene expression of TNFα/NF-κB
signalling molecules in lung cancer cells. Human lung cancer cells were treated
with varying concentrations of lutein, and cell viability assays were conducted
to determine any cytotoxic effects of lutein treatment. The gene expression levels of TNFα, NF-κB, and
related signalling molecules were assessed using quantitative polymerase chain
reaction (qPCR). Result: The outcomes of the research demonstrate that lutein treatment
led to a concentration-dependent alteration in cell viability and the expression of genes involved in
the TNFα/NF-κB signalling pathway in lung cancer cells. Specifically, a
significant downregulation of TNFα and inhibition of NF-κB activation were
observed in response to lutein treatment. Furthermore, our findings suggest
that lutein exhibited significant anti-tumor effects on the lung cancer cells
within the concentrations tested. These findings suggest a potential role for
lutein in modulating the TNFα/NF-κB signalling pathway in lung cancer cells,
highlighting its potential as an adjunct in lung cancer therapy. Further
studies are warranted to elucidate the precise mechanisms underlying lutein's
effects and to assess its therapeutic potential in preclinical and clinical
settings.
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