Abstract:

The study explores the development of a Chitosan-Whitlockite (Ch-WH) composite scaffold for bone tissue engineering. Chitosan, a natural polymer, and Whitlockite (WH), a calcium-magnesium phosphate mineral, are combined to leverage their individual properties. The scaffold was synthesized and characterized using X-ray diffraction (XRD) to confirm crystalline structure, Fourier-transform infrared spectroscopy (FTIR) for chemical interactions, scanning electron microscopy (SEM) for surface morphology and porosity, and energy-dispersive X-ray spectroscopy (EDS) to verify elemental composition. In vitro evaluations using human osteoblast-like cells demonstrated strong biocompatibility, with the scaffold supporting cell adhesion, proliferation, and differentiation. The addition of magnesium from Whitlockite enhanced osteoblast activity and bone mineralization. The scaffold also exhibited antibacterial properties, minimizing infection risks. With its biocompatibility, bioactivity, and antibacterial potential, the Ch-WH composite scaffold presents a promising solution for bone tissue engineering, warranting further exploration for clinical applications.

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