Abstract:

Burn patients suffer from breach of the protective skin layer which would lead to the association of complications including nosocomial bacterial infection. Klebsiella pneumoniae and Proteus mirabilis are among the most common bacterial infections. They are characterised by the expression of virulence factors leading to multi-drug resistance (MDR) the present study aimed to identify the association of virulence genes with burn bacterial infection. After a fire broke out in an accident in an Al-Hamdaniya town (Iraq), a total of 250 clinical isolates (swabs) were collected from patients. Identification of 184 (73.6%) and P. mirabilis 66 (26.4%) were made as the commonest Gram-negative bacteria to be studied. All isolates were carried out depending on microscopic examination, cultures, and genetic technique. A susceptibility test was achieved for all clinical isolates using the disk diffusion method. Ten antibiotics disks (imipenem, gentamycin, cefotaxime, sulphamethoxazole, pipracillin, cefixime, amoxycillin, trimethoprim, ciprofloxacin, and amikacin. For both K. pneumoniae and P. mirabilis, sensitivity was the highest with IMP (65% and 100%, respectively and lowest with PRL (0% and 4.6%, respectively). expressed mrkD, uge, fimH-, bla CTXM-1, and bla NDM-1, while the sample of P. mirabilis expressed aclb, bla TEM, atpD, ureC, rsbA, zabA, bla CTX, and bla OXA-1, these genes supposed that they evade the antibiotic therapy and thereby commence antibiotic-resistance. This study observed that some Iraqi isolates contain many different genes for both K. pneumoniae and P. mirabilis the causative agent of burn wound infections. These bacteria were resistant to multiple drugs with the highest sensitivity being associated with imipenem and the lowest with piperacillin.

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