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Molecular Approach to Identify Anti-inflammatory Potential of Stevioside in HFD-induced Type 2 Diabetic Rats: Evidence From in Vivo StudyAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art001
Molecular Approach to Identify Anti-inflammatory Potential of Stevioside in HFD-induced Type 2 Diabetic Rats: Evidence From in Vivo Study
Abstract:
Stevioside is a natural sweetener derived from the leaves of the Stevia rebaudiana plant. It has gained popularity as a sugar substitute due to its intense sweetness without adding calories or affecting blood sugar levels, making it a suitable option for people with diabetes or those looking to reduce their sugar intake. Studies have shown that stevioside has glucose lowering effects. Previous studies have shown that it has significant role in skeletal muscle but its role on expression of inflammatory signaling molecules in adipose tissue against high diet and sucrose-induced type-2 diabetes in experimental rats is yet to be done. The current research was undertaken to investigate if stevioside could also exert its antidiabetic effects by circumventing adipocyte induced inflammation, a key driving factor for insulin resistance in obese individuals. Effective dose of stevioside (20 mg/kg b.wt) was administered orally for 45 days to high fat diet and sucrose induced type-2 diabetic rats. Interestingly, stevioside treatment restores the elevated serum levels of proinflammatory cytokines including tumor necrosis factor-α (TNF-α) and sterol regulatory element binding protein-1c (SREBP-1c) and enhances Peroxisome Proliferator–activated receptor-γ (PPAR-γ) in adipocytes of diabetic rats. The gene expression of IR, GLUT4 and PPAR-γ mRNA were also significantly activated in stevioside treated groups but reduced IL-1 beta, IlL-6, IKKB, TNF-alpha and NFkB mRNA expression in diabetic adipose tissue. More importantly, stevioside acts very effectively as metformin to circumvent inflammation and insulin resistance in diabetic rats. Our results clearly show that stevioside inhibits obesity induced insulin resistance by ameliorating the inflammatory events and upregulating insulin signalling molecules.Keywords: Stevioside, HFD-T2DM, pro inflammation, insulin signalling; adipose tissue, obesity; signaling pathways; Therapautics.Molecular Approach to Identify Anti-inflammatory Potential of Stevioside in HFD-induced Type 2 Diabetic Rats: Evidence From in Vivo Study
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Sativoside Mitigates High-Fat Diet-Induced Inflammation and Type-2 Diabetes in Adipose Tissue of Wistar RatsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art002
Sativoside Mitigates High-Fat Diet-Induced Inflammation and Type-2 Diabetes in Adipose Tissue of Wistar Rats
Abstract:
This study aimed to investigate the impact of Stevioside, on the biochemical changes in high-fat diet-fed Wistar rats. Adult male Wistar rats were induced into a diabetic state through the administration of a high-fat diet and sucrose for 60 days, followed by oral administration of stevioside (20 mg/kg/day) for 45 days. Various parameters, including fasting blood glucose, oral glucose tolerance, insulin, insulin tolerance, liver function (ALT, AST, ALP), kidney function (urea and creatinine), and lipid profiles (TC, TG, FFA, HDL-c and LDL-c), serum adipokines levels such as adiponectin, leptin, resistin were assessed. Stevioside treatment notably improved glucose and insulin tolerances in diabetic rats and normalized their elevated levels of fasting blood glucose, serum insulin, and lipid profile. In the high-fat diet-induced type 2 diabetes rat model, Stevioside effectively restored the altered blood serum levels, demonstrating efficacy comparable to that of metformin. Therefore, Stevioside displays promise as a potential phytomedicine for managing type 2 diabetes mellitus.Keywords: High-fat diet, Insulin tolerance, Type-2 diabetes, Stevia rebaudiana.Sativoside Mitigates High-Fat Diet-Induced Inflammation and Type-2 Diabetes in Adipose Tissue of Wistar Rats
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Synthesis, Characterisation, and in Vitro Biocompatibility Studies of Selenium Nanoparticles Synthesized using Hybanthus Enneaspermus Plant Extract for Potential Biomedical ApplicationsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art003
Synthesis, Characterisation, and in Vitro Biocompatibility Studies of Selenium Nanoparticles Synthesized using Hybanthus Enneaspermus Plant Extract for Potential Biomedical Applications
Abstract:
Hybanthus enneaspermus (HE) is a traditional medicinal plant used for treating various disease conditions. Selenium nanoparticles (SeNPs) possess various properties such as anticancer, antioxidant, etc. The objective of the present study is to conduct green synthesis of selenium nanoparticles using Hybanthus enneaspermus(HE) and evaluate their biocompatibility. Leaves of HE are utilized for synthesizing SeNPs. Characterization studies of HE-SeNPs are carried out using UV spectrophotometry, FT-IR spectroscopy, and SEM. To check the biocompatibility, hemolytic assay, and Annexin V-PI assays are carried out. A change in color is observed after the addition of sodium selenite to the leaf extract. UV spectrophotometry gives a peak at 271 nm confirming the synthesis of SeNPs. FT-IR gives peaks at 3224, 1565, 1399, 1078, 784, and 717 cm-1 with a fingerprint of 3500 - 1000 cm-1. SEM analysis shows the spherical morphology of the SeNPs. HE-SeNPs at lower concentrations cause less hemolysis. However, HE-SeNPs are found to be less biocompatible, so further studies are needed to confirm their biocompatible nature. SeNPs synthesized from HE can be ideal for biomedical applications but further studies are required to check its biocompatibility.
Keywords: SeNPs, Hybanthus enneaspermus, green synthesis, biocompatibility.Synthesis, Characterisation, and in Vitro Biocompatibility Studies of Selenium Nanoparticles Synthesized using Hybanthus Enneaspermus Plant Extract for Potential Biomedical Applications
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One Pot Synthesis of Colloidal Zirconium Nanoparticles using Orthosiphon Stamineus Leaf Extract for Potential Bone Tissue Engineering ApplicationsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art004
One Pot Synthesis of Colloidal Zirconium Nanoparticles using Orthosiphon Stamineus Leaf Extract for Potential Bone Tissue Engineering Applications
Abstract:
Zirconium (ZrO2) is a metal oxide nanoparticles (NPs) possessing antimicrobial, antifungal, antioxidant, biosensing, biocompatibility, and anticancer activities. Due to their unique properties, ZrNPs can be used for multiple biomedical applications. Orthosiphon stamineus (OS) is a perennial medicinal herb with potent bioactive constituents. Traditionally, it was used in treating rheumatism, epilepsy, jaundice, hepatitis, etc, Hence OS could be used as a capping agent for synthesizing ZrO2NPs. The current study aimed to synthesize ZrO2NPs using a green source like Orthosiphon stamineus(OS) leaves extract and characterized using UV spectrophotometry, FTIR, SEM, EDX, and their biocompatibility was tested using Annexin V apoptosis assay. Milky precipitation formed, followed by the addition of OS extract to the aqueous solution of Zirconium oxychloride Octahydrate, revealed the formation of ZrO2NPs was further confirmed by the maximum absorbance at 296 nm in the UV-Vis spectrum. The peaks in the fingerprint region of FTIR revealed the presence of the functional groups of the phytoconstituents, confirming the capping. Apoptosis assay revealing the strong biocompatibility of ZrO2NPs towards peripheral blood mononuclear cells with 77.64% cell viability. From the apoptosis assay, it was evident that ZrO2NPs are less cytotoxic, indicating their applicability for medicinal applications. However further studies are required to validate its actions on bone tissue engineering.
Keywords: Green synthesis, Leaf extract, Nanoparticles, Cytotoxicity.One Pot Synthesis of Colloidal Zirconium Nanoparticles using Orthosiphon Stamineus Leaf Extract for Potential Bone Tissue Engineering Applications
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[10] Sushanthi, S., Srisakthi, D., Meignana Arumugham, I., Pradeepkumar, R., and Rajeshkumar, S. 2021. Vernonia Amygdalina Mediated Copper Nanoparticles and its Characterization and Antimicrobial Activity - An In Vitro Study. Int J Dentistry Oral Sci., 8 (7): 3330–3334.
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[14] Maliael, M. T., Jain, R. K., and Srirengalakshmi, M. 2022. Effect of nanoparticle coatings on frictional resistance of orthodontic archwires: a systematic review and meta-analysis. World J. Dent, 13 (4): 417–424.
[15] Nasim, I., Jabin, Z., Kumar, S. R., and Vishnupriya, V. 2022. Green synthesis of calcium hydroxide-coated silver nanoparticles using Andrographis paniculata and Ocimum sanctum Linn. leaf extracts: An antimicrobial and cytotoxic activity. J. Conserv. Dent. 25 (4): 369–374.
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[23] Rajeshkumar, S., and Lakshmi, S. (2021). Anticariogenic activity of silver nanoparticles synthesized using fresh leaves extract of kalanchoe pinnata. Int J Dentistry Oral Sci., 8 (7): 2985–2987.
[24] Nasim, I., Rajesh Kumar, S., Vishnupriya, V., and Jabin, Z. 2020. Cytotoxicity and anti-microbial analysis of silver and graphene oxide bio nanoparticles. Bioinformation, 16 (11): 831–836.
[25] Nasim, I., Rajeshkumar, S., and Vishnupriya, V. 2021. Green synthesis of reduced graphene oxide nanoparticles, its characterization, and antimicrobial properties against common oral pathogens. Int J Dentistry Oral Sci., 8 (2): 1670–1675.
[26] Sneka, and Santhakumar, P. 2021. Antibacterial Activity of Selenium Nanoparticles extracted from
Capparis decidua against Escherichia coli and Lactobacillus Species. J. Adv. Pharm. Technol. Res. 14 (8): 4452–4454.[27] Rieshy, V., Priya, J., Arivarasu, L., and Kumar, S. R. 2020. Enhanced Antimicrobial Activity Of Herbal Formulation Mediated Copper Nanoparticles Against Clinical Pathogens. Plant Cell Biotechnol. Mol. Biol. 21 (53-54): 52-56.
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Curcumin Coated Orthosiphon Stamineus Leaf Extract Based Selenium Nanoparticle for Potential Tissue Engineering ApplicationsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art005
Curcumin Coated Orthosiphon Stamineus Leaf Extract Based Selenium Nanoparticle for Potential Tissue Engineering Applications
Abstract:
Curcumin is a type of polyphenol phytochemical that is bright yellow in colour and produced by the plant Curcuma longa. Despite its pharmacological properties, curcumin has low bioavailability, poor solubility, and undergoes rapid degradation. Nanoparticles (NPs) are used as a nanocarrier for drug delivery, to improve the stability and pharmacokinetics of the drug. Therefore, by coating curcumin over selenium NPs (SeNPs), the bioactivity, bioavailability, stability, and may increase the solubility of curcumin of SeNPs. This study aimed to synthesize the SeNPs from Orthosiphon stamineus leaf extract and coat it with curcumin and to characterize it and check its biocompatibility. Biosynthesis of SeNPs was carried out using plant extract of Evolvulus alsinoides and characterized using UV spectrophotometer, FT-IR, and SEM. Annexin V PI apoptotic and Hemolytic assay were used for checking biocompatibility. The UV-Vis spectrum gave a strong peak at 265 and 423 nm at various time intervals, indicating the SeNPs formation. Similarly, FT-IR has strong absorption bands at 3279, 1284, 1072, 1028, and cm−1 with wavelengths ranging from 4000-500 cm-1. SEM analysis of biosynthesized SeNPs showed a spherical shape. Our results suggest that curcumin-coated SeNPs possess greater biocompatibility towards PBMCs which was evaluated by Annexin V - PI assay and erythrocytes by hemolytic studies. Curcumin-coated Selenium nanoparticles were successfully synthesized by the biological method using leaf extract of Orthosiphon stamineus and reported as biocompatible using Flow cytometry. But a more detailed study should be done for implementing it in tissue engineering.
Keywords: Selenium nanoparticle, Curcumin, Orthosiphon stamineus, Tissue Engineering.Curcumin Coated Orthosiphon Stamineus Leaf Extract Based Selenium Nanoparticle for Potential Tissue Engineering Applications
References:
[1] Nabi, F., Arain, M. A., Hassan, F., Umar, M., Rajput, N., Alagawany, M., … Liu, J. 2020. Nutraceutical role of selenium nanoparticles in poultry nutrition: a review. Worlds Poult. Sci. J. 76 (3): 459–471.
[2] Pandiyan, I., Sri, S. D., Indiran, M. A., Rathinavelu, P. K., Prabakar, J., and Rajeshkumar, S. (2022). Antioxidant, anti-inflammatory activity of Thymus vulgaris-mediated selenium nanoparticles: An in vitro study. J. Conserv. Dent. 25 (3): 241–245.
[3] Sushanthi, S., Srisakthi, D., MeignanaArumugham, I., Pradeepkumar, R., and Rajeshkumar, S. 2021. Vernonia Amygdalina Mediated Copper Nanoparticles and its Characterization and Antimicrobial Activity - An In Vitro Study. Int J Dent. Oral Sci., 8 (7): 3330–3334.
[4] Maiyo, F., and Singh, M. 2017. Selenium nanoparticles: potential in cancer gene and drug delivery. Nanomedicine , 12 (9): 1075–1089.
[5] Maliael, M. T., Jain, R. K., and Srirengalakshmi, M. 2022. Effect of nanoparticle coatings on frictional resistance of orthodontic archwires: a systematic review and meta-analysis. World J. Dent, 13(4), 417–424.
[6] Nasim, I., Jabin, Z., Kumar, S. R., and Vishnupriya, V. (2022). Green synthesis of calcium hydroxide-coated silver nanoparticles using Andrographis paniculata and Ocimum sanctum Linn. leaf extracts: An antimicrobial and cytotoxic activity. JJ. Conserv. Dent. 25 (4): 369–374.
[7] Kamath, K. A., Nasim, I., and Rajeshkumar, S. (2020). Evaluation of the re-mineralization capacity of a gold nanoparticle-based dental varnish: An in vitro study. J. Conserv. Dent. 23 (4): 390–394.
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Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antioxidant Activity EvaluationAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art006
Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antioxidant Activity Evaluation
Abstract:
Gold continues to be one of the oldest dental restorative materials which has been used for dental repairs for more than 4000 years and remains an important metal in the dental sector. In a world where the importance of nanoparticles has been well established and its preparation has become much easier, it is important to analyse if these nanoparticles can be extracted from a plant based source as well. Along with its extraction, assessment of each property of the nanoparticle is essential. A few ingredients used in Ayurveda which can also be found being used in almost every household is pepper and eucalyptus and over the years, its importance has remained constant, if not showing an increase. The aim of this study was to extract gold nanoparticles using Eucalyptus and Piper longum and evaluate the antioxidant activity of the derived gold nanoparticles. Preparation of plant extract was done following which, extraction of gold nanoparticles was performed. Antioxidant properties of the gold nanoparticles were tested by a DPPH assay method and compared against the antioxidant gold standard, butylated hydroxytoluene (BHT). The percentage of absorbance was calculated and data was analyzed. The results demonstrated the presence of elemental gold. Both, plant extract derived AuNPs exhibited significant free-radical scavenging activity indicating that they possess antioxidant effects.
Keywords - Eucalyptus, antioxidant, Nanoparticles, Novel technique, Piper longum.Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antioxidant Activity Evaluation
References:
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[24] Chokkattu JJ, Mary DJ, Shanmugam R, Neeharika S. Embryonic Toxicology Evaluation of Ginger-and Clove-mediated Titanium Oxide Nanoparticles-based Dental Varnish with Zebrafish. The Journal of Contemporary Dental Practice. 2023 Mar 17;23(11):1157-62.
[25] Niveda Rajeshwaran JR, Rajeshkumar S. Evaluation of Antioxidant and Anti-Inflammatory Activity of Grape Seed Oil Infused with Silver Nano-particles an In Vitro Study. Int J Dentistry Oral Sci. 2021 Jul 15;8(7):3318-22.
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Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antiinflammatory Activity EvaluationAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art007
Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antiinflammatory Activity Evaluation
Abstract:
Gold is the oldest dental restorative material, used for dental repairs for more than 4000 years and remains an important metal included in the dental sector. In a world where nanoparticle importance has been well established and preparation of nanoparticles has become much easier, it is important to assess if these nanoparticles can be extracted from plants as well. Along with its extraction, analysis of each property of the nanoparticle is essential. Pepper and eucalyptus remain two of the most important ingredients used in ayurveda and can be easily found in every household. The aim of this study was to extract gold nanoparticles using Eucalyptus and Piper longum and evaluate the antibacterial activity of the derived gold nanoparticles. Preparation of plant extract was done following which, extraction of gold nanoparticles was performed. Antiinflammatory properties of the gold nanoparticles were tested by albumin denaturation method and compared against the anti-inflammatory gold standard, Diclofenac sodium. The protein denaturation levels were measured, and the data was compiled. From this study, it can be concluded that gold nanoparticles derived from pepper and eucalyptus can be used as a potential source of anti-inflammatories.
Keywords - Eucalyptus, antiinflammatory, Nanoparticles, novel technique, Gold, Piper longum.Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antiinflammatory Activity Evaluation
References:
[1] Chen PC, Mwakwari SC, Oyelere AK. Gold nanoparticles: from nanomedicine to nanosensing. Nanotechnology, science, and applications. 2008; 1:45.
[2] Gielen M, Tiekink ER, editors. Metallotherapeutic drugs and metal-based diagnostic agents: the use of metals in medicine. John Wiley & Sons; 2005 Sep 1.
[3] Kumar PS, Pastoriza-Santos I, Rodríguez-González B, De Abajo FJ, Liz-Marzán LM. High-yield synthesis and optical response of gold nanostars. Nanotechnology. 2007 Nov 29;19(1):015606.
[4] Edelman ER, Seifert P, Groothuis A, Morss A, Bornstein D, Rogers C. Gold-coated NIR stents in porcine coronary arteries. Circulation. 2001 Jan 23;103(3):429-34.
[5] Svedman C, Tillman C, Gustavsson CG, Möller H, Frennby B, Bruze M. Contact allergy to gold in patients with gold‐plated intracoronary stents. Contact Dermatitis. 2005 Apr;52(4):192-6.
[6] Thelen A, Bauknecht HC, Asbach P, Schrom T. Behavior of metal implants used in ENT surgery in 7 Tesla magnetic resonance imaging. European Archives of Oto-Rhino-Laryngology and Head & Neck. 2006 Oct;263(10):900-5.
[7] Demann ET, Stein PS, Haubenreich JE. Gold as an implant in medicine and dentistry. Journal of long-term effects of medical implants. 2005;15(6).
[8] Wersäll PJ, Blomgren H, Pisa P, Lax I, Kälkner KM, Svedman C. Regression of non-irradiated metastases after extracranial stereotactic radiotherapy in metastatic renal cell carcinoma. Acta oncologica. 2006 Jan 1;45(4):493-7.
[9] Shaw CF. Gold-based therapeutic agents. Chemical reviews. 1999 Sep 8;99(9):2589-600.
[10] Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang Y, Shao W, He N, Hong J. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology. 2007 Feb 6;18(10):105104.
[11] Wright DC, German RM, Gallant RF. Materials Science: Copper and Silver Corrosion Activity in Crown and Bridge Alloys. Journal of Dental Research. 1981 Apr;60(4):809-14.
[12] Knosp H, Holliday RJ, Corti CW. Gold in dentistry alloys, uses and performance. Gold bulletin. 2003 Sep;36(3):93-102
[13] Rieshy, V., Priya, J., Arivarasu, L., Kumar, S. R., & Devi, G. (2020). Enhanced antimicrobial activity of herbal formulation mediated copper nanoparticles against clinical pathogens. Plant cell biotechnology and molecular biology, 21(53-54), 52–56.
[14] Kishore, S. O. G., Priya, A. J., Narayanan, L., Kumar, S. R., & Devi, G. (2020). Controlling of oral pathogens using turmeric and tulsi herbal formulation mediated copper nanoparticles. Plant cell biotechnology and molecular biology, 21(53-54), 33–37.
[15] Sneka S, Preetha Santhakumar. Antibacterial Activity of Selenium Nanoparticles extracted from Capparis decidua against Escherichia coli and Lactobacillus Species. Research Journal of Pharmacy and Technology. 2021; 14(8):4452-4. doi: 10.52711/0974-360X.2021.00773
[16] Roshan, A., Jothipriya, A., Arivarasu, L., Kumar, R., & Devi, G. (2020). Antifungal activity of tulsi and turmeric assisted copper nano particles. Plant cell biotechnology and molecular biology, 21(27-28), 9–13.
[17] Iffat Nasim, S. Rajeshkumar, V Vishnupriya. Green Synthesis of Reduced Graphene Oxide Nanoparticles, Its Characterization and Antimicrobial Properties against Common Oral Pathogens. Int J Dentistry Oral Sci. 2021;8(2):1670-1675
[18] Nasim I, Kumar SR, Vishnupriya V, Jabin Z. Cytotoxicity and anti-microbial analysis of silver and graphene oxide bio nanoparticles. Bioinformation. 2020;16(11):831.
[19] Rajeshkumar S, Lakshmi T. Anticariogenic Activity Of Silver Nanoparticles Synthesized Using Fresh Leaves Extract Of Kalanchoe Pinnata. Int J Dentistry Oral Sci. 2021 Jul 2;8(7):2985-7.
[20] Rajeshkumar S, Jayapriya J, Lakshmi T. A Review on plant mediated selenium nanoparticles and its applications: Selenium nanoparticles. Journal of Population Therapeutics and Clinical Pharmacology. 2021;28(2).
[21] Kamath KA, Nasim I, Rajeshkumar S. Evaluation of the re-mineralization capacity of a gold nanoparticle-based dental varnish: An in vitro study. Journal of conservative dentistry: JCD. 2020 Jul;23(4):390.
[22] Maliael MT, Jain RK, Srirengalakshmi M. Effect of nanoparticle coatings on frictional resistance of orthodontic archwires: a systematic review and meta-analysis. World. 2022;13(4).
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Effect of Diosmin on The Expression of Epithelial-Mesenchymal Transition Signaling Molecules in Ndea-Induced Hepato-Cellular Carcinoma in Experimental RatsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art008
Effect of Diosmin on The Expression of Epithelial-Mesenchymal Transition Signaling Molecules in Ndea-Induced Hepato-Cellular Carcinoma in Experimental Rats
Abstract:
Hepatocellular carcinoma (HCC) is a primary liver cancer, distinct from other cancers originating in other organs. Previous study demonstrated that diosmin exhibits anticancer effects by influencing the expression of apoptotic signaling molecules in NDEA-induced hepatocellular carcinoma in rats. However, its impact on the epithelial-mesenchymal transition (EMT) signaling pathway, crucial in liver cancer progression, remains unknown. The research aimed to investigate diosmin’s effects on EMT signaling molecule expression in NDEA-induced hepatocellular carcinoma in rats. In this experiment, adult male albino rats were categorized into three groups: a control group, NDEA-induced hepatocellular carcinogenic rats and rats with HCC treated with diosmin orally for 28 days. Liver function markers (AST and ALT) were done by biochemical analysis while mNRA expression analysis of EMT-signaling molecules ( E-cadherin and vimentin) were analyzed by Real Time-RT-PCR analysis. One-Way-ANOVA was used for the statistical analysis and significance was considered at p<0.05. Diosmin treatment resulted in a significant decrease in liver function markers compared to the control group (p<0.05). Moreover, diosmin administration led to a notable reduction in mRNA levels of EMT signaling molecules, specifically E-cadherin and vimentin, indicating its potential chemopreventive role against liver cancer. Findings of the present study concludes that diosmin, an alkaloid, may merge as a promising candidate for hepatocellular carcinoma treatment based on its demonstrated efficacy in this experimental model.Keywords: Novel method, Hepatocellular carcinoma, EMT signaling, diosmin, wistar rats, liver function, innovative technique.Effect of Diosmin on The Expression of Epithelial-Mesenchymal Transition Signaling Molecules in Ndea-Induced Hepato-Cellular Carcinoma in Experimental Rats
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Fabrication, Characterization, Antibacterial and Biocompatibility Studies of Graphene Oxide Loaded Alginate Chitosan Scaffolds for Potential Biomedical ApplicationsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art009
Fabrication, Characterization, Antibacterial and Biocompatibility Studies of Graphene Oxide Loaded Alginate Chitosan Scaffolds for Potential Biomedical Applications
Abstract:
Graphene oxide nanomaterial possesses greater biocompatibility. Chitosan alginate is produced from chitin by deacetylation, it is a biodegradable and biocompatible biomaterial. Graphene has a large specific surface area that enhances the antibacterial effect by enabling biocompatible interactions with bacterial membranes. To fabricate a biocompatible graphene oxide loaded alginate chitosan scaffold for potential biomedical uses and perform the characterization, antibacterial, and biocompatibility properties of ALG-CHI-GO scaffolds. The scaffolds were prepared by mixing the solution of ChitosanHCl (5 %) and graphene oxide-oxidized alginate (10 %). This gelled mixture is freeze-dried (lyophilization) to form the scaffold and is later characterized using FTIR and SEM, The scaffolds were then tested for in biocompatibility towards peripheral blood mononuclear cells and antibacterial properties against Enterococcus faecalis and Streptococcus mutans. The biocompatibility towards peripheral blood mononuclear was checked using the annexin V PI assay. To conclude that the fabricated Graphene oxide loaded chitosan alginate scaffold was found to be biocompatible and showed antibacterial properties.
Keywords: Antibacterial activity, Biocompatibility, Chitosan alginate scaffolds, Graphene oxide.Fabrication, Characterization, Antibacterial and Biocompatibility Studies of Graphene Oxide Loaded Alginate Chitosan Scaffolds for Potential Biomedical Applications
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Biogenic Selenium Nanoparticles Loaded Alginate-Gelatin Scaffolds for Potential Tissue Engineering ApplicationsAuthor: Vishnu Priya VeeraraghavanDOI: 10.21522/TIJPH.2013.SE.23.01.Art010
Biogenic Selenium Nanoparticles Loaded Alginate-Gelatin Scaffolds for Potential Tissue Engineering Applications
Abstract:
Selenium nanoparticles (SeNPs) were reported for its anticancer and antimicrobial properties. Alginate and gelatin scaffolds can act as an important biomaterial, more specifically in bone tissue engineering. Green synthesis of SeNPs from Luffa cylindrica (LC) and loading of SeNPS with alginate-gelatin scaffold and to check its biocompatibility. The SeNPs were prepared via the green synthesis method and loaded into an alginate-gelatin scaffold. Characterization studies such as UV-Vis spectroscopy, FTIR, and SEM were carried out in LC-SeNPs and Se-NPs loaded scaffold. The hydrophilicity of the scaffolds was determined using water contact angle measurements. Annexin V PI assay was conducted to determine the biocompatible nature of prepared SeNPs-loaded alginate-gelatin scaffolds. The UV-VIS spectrum gave an intense peak at 266 and 384 nm, whereas the FTIR gave a strong peak at 3500-500 cm-1 fingerprint regions. SEM images showed flower-shaped LC-SeNPs and their distribution of SeNPs on the surface of alginate-gelatin scaffolds. water contact angle measurement was found to be 29.21°. Cell viability results showed 78.11% viable cells following treatment with alg-gel-Se scaffold, revealing its biocompatibility towards peripheral blood mononuclear cells. Overall, it could be concluded that the SeNP-loaded alg-gel scaffold is a promising candidate for tissue engineering, but further studies are required to confirm its potential role.
Keywords- Selenium nanoparticles, Alginate-gelatin scaffolds, tissue engineering, biocompatibility.Biogenic Selenium Nanoparticles Loaded Alginate-Gelatin Scaffolds for Potential Tissue Engineering Applications
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