Abstract:

The study aimed to describe the prevalence of geriatric syndromes among older adults living in the community. In addition, we evaluated the relationship between frailty and socio-demographic factors and other geriatric syndromes in the Central Mahalapye sub-district, Botswana. This cross-sectional study recruited 414 older adults from the community using the Botswana Pension Office’s roster. We employed a systematic random selection method to recruit 160 older adults in three main villages and we randomly chose 150 elderly individuals in 10 research villages also randomly selected using Microsoft Office. We used the brief-assessment-tool-for-comprehensive-geriatric-assessment to collect information about geriatric syndromes. We conducted a multivariate analysis using binary logistic regression to determine the relationship between the independent variables (sociodemographic factors and other geriatric disorders) and frailty. Most of older adults had geriatric syndromes, particularly Instrumental-Activity-of-Daily-Living impairment (n= 328, 79.2%). About half of the participants were at nutrition risk (n= 261, 63.0%)), had a mood disorder/ depression (n= 237, 57.2%), had visual impairment (n= 245, 59.2%), or were frail (n= 219, 47.1%). In approximately one-third of cases, participants reported hearing impairment (n= 127, 30.7%) or having experienced at least one fall in the past year (n= 117, 28.3%). Factors such as high education level, living with a partner, urinary incontinence, and cognitive impairment influenced frailty (p<0.05). We intend to explore the potential impact of family size (despite pension allowance), alcohol consumption, oral health issues, and tooth loss on the nutritional vulnerability of elderly individuals in Sub-Saharan Africa, with a particular emphasis on Botswana.

References:

Abstract:

This study investigates the presence and risks of common industrial antimicrobial agents used, in the vicinity of Vellore's leather industrial hub, Tamil Nadu, India. Water and sediment samples (n=25 each) were collected from two major tannery clusters, Ranipet and Ambur-Vaniyambadi. Using GC-MS and UPLC-DAD analyses, we attempted to detect six major antimicrobial classes such as chlorinated phenols, 2-(Thiocyanomethylthio) benzothiazole (TCMTB), ortho phenyl phenol (OPP), Para chloro m-cresol (PCMC), triclosan (TCS) and n-Octyl-isothiazolinone (n-OIT). Among them, only TCMTB, OPP, and PCMK were recorded in water samples, whereas in sediment samples none of the antimicrobials were found. Detection frequencies in water were 24%, with PCMC levels reaching up to 910 µg/L, TCMTB at 45.3 µg/L, and OPP at 821.3 µg/L. A comparative analysis with global studies showed that contaminant levels in this study are significantly higher than in other country reports. Human health risk assessment, based on USEPA methods, revealed a risk quotient (RQ) of 5.200 for PCMC, indicating a potential health risk, whereas TCMTB and OPP presented lower risks. Environmental risk assessment indicated hazard quotients (HQ) >1 for all detected antimicrobials, suggesting significant ecological risks. The study highlights the need for stringent regulations, improved industrial practices, and better wastewater treatment infrastructure. Continuous monitoring and more comprehensive studies with increased sampling are essential to develop strategies for reducing contaminants from leather industry runoff. These measures are crucial for protecting human health and aquatic ecosystems in the region.

References:

[4]. Morrissey, I., Oggioni, M. R., Knight, D., Curiao, T., Coque, T., Kalkanci, A., et al. 2014, Evaluation of Epidemiological Cut-Off Values Indicates that Biocide Resistant Subpopulations are Uncommon in Natural Isolates of Clinically Relevant Microorganisms. PLoS ONE, 9(1). https://doi.org/10.1371/journal.pone.0086669

[5]. Government of Tamil Nadu. 2020, Tamil Nadu's Economic Contribution to India's GDP. State Government Publications. Retrieved from https://www.tn.gov.in/documents/economic-contribution. Clasen, B., et al. 2018, Determination of Selected Pesticides and Biocides in Surface Water Using Multi-Residue Analysis by UHPLC-MS/MS. Analytical and Bioanalytical Chemistry, 410(25), 6375-6388. https://doi.org/10.1007/s00216-018-1339-5Guardiola, F. A., Cuesta, A., Meseguer, J., & Esteban, M. A. (2012, February). Risks of Using Antifouling Biocides in Aquaculture. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms13021541Igbinosa et al., 2013

[6]. Vázquez, A., Alvarado, L., Lázaro, I., Cruz, R., Nava, J. L., & Rodríguez-Torres, I. 2018, A Comparative Analysis of 2-(Thiocyanomethylthio)- Benzothiazole Degradation Using Electro-Fenton and Anodic Oxidation on a Boron-Doped Diamond Electrode. International Journal of Photoenergy, 2018. https://doi.org/10.1155/2018/5290975

[7]. Liao, C., Kim, U. J., & Kannan, K. 2018, May 1. A Review of Environmental Occurrence, Fate, Exposure, and Toxicity of Benzothiazoles. Environmental Science and Technology. American Chemical Society. https://doi.org/10.1021/acs.est.7b05493

[8]. Coelhan, M., Bromig, K. H., Glas, K., & Roberts, A. L., 2006, Determination and Levels of the Biocide Ortho-Phenylphenol in Canned Beers from Different Countries. Journal of Agricultural and Food Chemistry, 54(16), 5731–5735. https://doi.org/10.1021/jf060743p

[9]. Bomhard, E. M., Brendler-Schwaab, S. Y., Freyberger, A., Herbold, B. A., Leser, K. H., & Richter, M., 2002, O-Phenylphenol and its Sodium and Potassium Salts: A Toxicological Assessment. Critical Reviews in Toxicology (Vol. 32).

[10]. Pizarro, A. H., Molina, C. B., Munoz, M., de Pedro, Z. M., Menendez, N., & Rodriguez, J. J., 2017, Combining HDC and CWPO for the Removal of p-chloro-m-cresol from Water Under Ambient-like Conditions. Applied Catalysis B: Environmental, 216, 20–29. https://doi.org/10.1016/j.apcatb.2017.05.052

[11]. Dhillon, G. S., Kaur, S., Pulicharla, R., Brar, S. K., Cledón, M., Verma, M., & Surampalli, R. Y., 2015, May 22. Triclosan: Current Status, Occurrence, Environmental Risks and Bioaccumulation Potential. International Journal of Environmental Research and Public Health. MDPI. https://doi.org/10.3390/ijerph120505657

[12]. Dar, O. I., Aslam, R., Pan, D., Sharma, S., Andotra, M., Kaur, A., et al. 2022, February 1. Source, Bioaccumulation, Degradability and Toxicity of Triclosan in Aquatic Environments: A review. Environmental Technology and Innovation. Elsevier B.V. https://doi.org/10.1016/j.eti.2021.102122

[13]. Kim, D., Kim, E. H., Choi, S., Lim, K. M., Tie, L., Majid, A., & Bae, O. N., 2021, A Commonly used Biocide 2-N-Octyl-4-Isothiazolin-3-One Induces Blood–Brain Barrier Dysfunction Via Cellular Thiol Modification and Mitochondrial Damage. International Journal of Molecular Sciences, 22(5), 1–17. https://doi.org/10.3390/ijms22052563

[14]. Zuccarello, P., Ferrante, M., Cristaldi, A., Copat, C., Grasso, A., Sangregorio, D., Fiore, M., Oliveri Conti, G., Reply for Comment on "Exposure To Microplastics (<10 μm) Associated to Plastic Bottles Mineral Water Consumption: The First Quantitative Study by Zuccarello et al. [Water Research 157 (2019) 365-371]". Water Res. 2019 Dec 1;166:115077. doi: 10.1016/j.watres.2019.115077. Epub 2019 Sep 13. PMID: 31546101.

[15]. Li, L. F., Zeng, X. B., Li, G. X., & Mei, X. R., 2014, Surface Water Quality Assessment in Beijing (China) Using GIS-Based Mapping and Multivariate Statistical Techniques. In Advanced Materials Research (Vols. 955–959, pp. 1514–1526). Trans Tech Publications, Ltd. https://doi.org/10.4028/www.scientific.net/amr.955-959.1514 Naidoo et al., 2021)

[16]. Hutton, M., & Shafahi, M., "Water Pollution Caused by Leather Industry: A Review." Proceedings of the ASME 2019 13th International Conference on Energy Sustainability Collocated with the ASME 2019 Heat Transfer Summer Conference. ASME 2019 13th International Conference on Energy Sustainability. Bellevue, Washington, USA. July 14–17, 2019. V001T10A002. ASME. https://doi.org/10.1115/ES2019-3949

[17]. Johnson, L., et al. 2022, "Assessment of Industrial Pollutants in Surface Water: A Case Study from Kentucky, USA." Environmental Monitoring and Assessment. DOI: 10.1007/s10661-021-08768-7

[18]. Hartono, D., et al. 2020, "Pollutant Levels in Surface Water Near Industrial Areas in Jakarta, Indonesia." Journal of Environmental Science and Technology, DOI: 10.1080/10934529.2020.1753142

[19]. Meneses, E. S., Arguelho, M. L. P. M., & Alves, J. P. H., 2005, Electroreduction of the Antifouling Agent TCMTB and its Electroanalytical Determination in Tannery Wastewaters. Talanta, 67(4), 682–685. https://doi.org/10.1016/j.talanta.2005.01.058

[20]. Hornung, M. W., Kosian, P. A., Haselman, J. T., Korte, J. J., Challis, K., Macherla, C., et al. 2015, In Vitro, Ex Vivo, and in Vivo Determination of Thyroid Hormone Modulating Activity of Benzothiazoles. Toxicological Sciences, 146(2), 254–264. https://doi.org/10.1093/toxsci/kfv090

[21]. Teschke, K., He-man, C., Wiens, M., Dimich-Ward, H., Hershler, R., Ostry, A., & Kelly, S. J., 1992, Recognizing Acute Health Effects of Substitute Fungicides: Are First-Aid Reports Effective? American Journal of Industrial Medicine (Vol. 21).

[22]. Morrissey, I., Oggioni, M. R., Knight, D., Curiao, T., Coque, T., Kalkanci, A., et al. 2014, Evaluation of Epidemiological Cut-Off Values Indicates that Biocide Resistant Sub Populations are Uncommon in Natural Isolates of Clinically Relevant Microorganisms. PLoS ONE, 9(1). https://doi.org/10.1371/journal.pone.0086669

[23]. USEPA 1984, US Environmental Protection Agency Report on “Health Effects Assessment Summary (HEAST) for Thiocyanomethylthio) Benzothiazole”. Retrieved June 24, 2024, from https://rais.ornl.gov/epa/heast/ThiocyanomethylthioBenzothiazole2.html

[24]. Lau, W. W. Y., & Stenstrom, M. K., 2005, Metals and Organic Micro-Pollutants in Stormwater Runoff from a Commercial Land use Area. Water Environment Research, 77(2), 149-160. DOI:10.2175/106143005X41664.

[25]. Bollmann, U. E., Tang, C., Eriksson, E., Jönsson, K., Vollertsen, J., & Bester, K., 2014, Biocides in Urban Wastewater Treatment Plant Influent at Dry and Wet Weather: Concentrations, Mass Flows and Possible Sources. Water Research, 60, 64-74. DOI: 10.1016/j.watres.2014.04.041.

[26]. Ramaswamy, B. R., Shanmugam, G., Velu, G., Rengarajan, B., & Larsson, D. G. J., 2011, GC-MS Analysis and Ecotoxicological Risk Assessment of Triclosan, Carbamazepine and Parabens in Indian Rivers. Journal of Hazardous Materials, 186(2-3), 1586-1593. DOI: 10.1016/j.jhazmat.2010.12.037.

[27]. Fent, K., Weston, A. A., & Caminada, D., 2006, Ecotoxicology of Human Pharmaceuticals. Aquatic Toxicology, 76(2), 122-159. DOI: 10.1016/j.aquatox.2005.09.009.

[28]. Lu, X., Yuan, D., Chen, Y., & Fung, J. C. H., 2021, Impacts of Urbanization and Long-Term Meteorological Variations on Global PM2.5 and its Associated Health Burden. Environmental Pollution, 270, 116003. https://doi.org/10.1016/j.envpol.2020.116003

[29]. Xu, W. H., Zhang, G., Wai, O. W. H., Zou, S. C., & Li, X. D., 2009, Transport and adsorption of Antibiotics by Marine Sediments In A Dynamic Environment. Journal of Soils and Sediments, 9(4), 364–373. https://doi.org/10.1007/s11368-009-0091-z

[30]. Zhang, Y., Zhao, Y.-G., Maqbool, F., & Hu, Y. 2022, Removal of antibiotics pollutants in wastewater by UV-based advanced oxidation processes: Influence of water matrix components, processes optimization and application: A review. Journal of Water Process Engineering, 45, 102496. https://doi.org/10.1016/j.jwpe.2021.102496

Abstract:

Asthma ranks among the top 20 chronic conditions for the global ranking of disability-adjusted life years in children and is the most prevalent chronic condition in children. In the mid-childhood ages of 5–14 years, it is among the top 10 causes. Parental asthma knowledge is a crucial influencer of management practices and disease control. To achieve guided self-management, education is one of the six essential features. Assessing parents' asthma-related knowledge (ARK) is a significant requirement for improving childhood asthma management. The primary objective of this study was to document the ARK of parents of children (0-18 years) with asthma and identify how their knowledge impacts the control of the medical condition in their children in Accra, Ghana. A short questionnaire was designed and implemented to gather relevant information for the study. We also determined the number of children using an asthma action plan. A total of 200 questionnaires were administered. The median age of the children in the study was 10.7 years, with a mean age of 10.0 years. Seventy-one per cent of the children were males. This variation is based on the standard population, where asthma in boys is nearly twice as significant as in girls before age 14. Asthma educators should pay attention to the level of education of the parents of asthmatic children. The result indicates a higher level of asthma control in children whose parents have a higher level of education. However, it is concerning that only 17.2% of children had an asthma action plan, highlighting the urgent need for action in this area.

References:

Abstract:

Booster vaccinations have become significant in developing herd immunity due to their ability to reduce mortality rates and the severe dangers of Covid-19. Some people support the government's program and believe that booster vaccines will help prevent Covid-19 during the epidemic. Some people, however, say they do not want booster vaccinations because they believe the first and second doses are adequate and do not want to receive additional doses. The purpose of this study is to investigate the Covid-19 vaccination booster dose policy acceptance among pregnant women. This cross-sectional study was conducted in March to April 2023. Participants were 185 pregnant women selected from several subdistrict in Malang. Instruments for data collection were a demographic questionnaire, an attitude questionnaire, a social media questionnaire, a role of health workers questionnaire, and questions about Covid-19 vaccine booster dose acceptance. Logistic regression analysis was used to assess the effects of the study variables on Covid-19 vaccine booster dose acceptance. In the regression model, the three factors of attitude (b= 0.30; SE=0.07; 95% CI= 0.16 to 0.44), social media (b= 0.47; SE=0.13; 95% CI= 0.22 to 0.73), and role of health workers (b= 0.29; SE=0.15; 95% CI= 0.01 to 0.58) had significant effects on Covid-19 vaccine booster dose acceptance (p< 0.05). Acceptance of the Covid-19 booster vaccines among pregnant women can be accelerated through their positive attitude towards the vaccination program, the proper use of social media, and the involvement of healthcare workers.

References:

[1]. Laupèze, B., Del Giudice, G., Doherty, M. T, Van der Most, R., 2021, Vaccination as a Preventative Measure Contributing to Immune Fitness. npj Vaccines, 6(1). Available from: https://dx.doi.org/10.1038/s41541-021-00354-z

[2]. Zhang, S., Yang, Z., Chen, Z. L., Yue, S. J., Zhang, S., Tang, Y. P., 2022, Why Does Covid-19 Continue to Spread Despite Mass Vaccination? Front Public Heal, 10(4).

[3]. Tiyo, B. T, Schmitz, G. J. H., Ortega, M. M., da Silva, L. T., de Almeida, A., Oshiro, T. M., et al., 2021, What Happens to the Immune System After Vaccination or Recovery From COVID-19? Life, 11(11).

[4]. Nugraha, R. R, Miranda, A. V., Ahmadi, A., Lucero-Prisno, D, E., 2021, Accelerating Indonesian COVID-19 Vaccination Rollout: A Critical Task Amid the Second Wave. Trop Med Health, 49(1). Available from: https://doi.org/10.1186/s41182-021-00367-3

[5]. Al Awaidy, S. T., Khatiwada, M., Castillo, S., Al Siyabi, H., Al Siyabi, A., Al Mukhaini. S., et al., 2022, Knowledge, Attitude, and Acceptability of COVID-19 Vaccine in Oman: A Cross-Sectional Study. Oman Med J, 37(3).

[6]. Rela, I. Z, Ramli, Z., Firihu, M.Z., Widayati, W., Awang, A. H., Nasaruddin, N., 2022, COVID-19 Risk Management and Stakeholder Action Strategies: Conceptual Frameworks for Community Resilience in the Context of Indonesia. Int J Environ Res Public Health, 19(15).

[7]. García-Botella, A., García-Lledó, A., Gómez-Pavón, J., Del Castillo, J. G., Hernández-Sampelayo, T., Martín-Delgado, M. C., et al., 2021, Booster or Additional Vaccination Doses in Patients Vaccinated Against COVID-19. Rev Esp Quimioter, 35(2):105–14.

[8]. Jung, J., 2021, Preparing for the Coronavirus Disease (COVID-19) Vaccination: Evidence, Plans, and Implications. J Korean Med Sci, 36(7):1–20.

[9]. Sarma, P., Kaur, H., Kumar, H., Mahendru, D., Avti, P., Bhattacharyya, A., et al., 2020, Virological and Clinical Cure in Covid-19 Patients Treated With Hydroxychloroquine: A Systematic Review and Meta-Analysis. J Med Virol, 92(7):776–85.

[10]. Atmar, R. L., Lyke, K. E., Deming, M. E., Jackson, L. A., Branche, A. R., El Sahly, H. M, et al., 2022, Homologous and Heterologous Covid-19 Booster Vaccinations. N Engl J Med, 386(11):1046–1057.

[11]. Costa Clemens, S. A., Weckx, L., Clemens, R., Almeida Mendes, A. V., Ramos Souza, A., Silveira, M. B. V., et al., 2022, Heterologous Versus Homologous Covid-19 Booster Vaccination in previous Recipients of Two Doses of Coronavac Covid-19 Vaccine in Brazil (RHH-001): A Phase 4, Non-Inferiority, Single Blind, Randomised Study. Lancet, 399(10324):521–9.

[12]. Cerqueira-Silva, T., Shah, S. A., Robertson, C., Sanchez, M., Katikireddi, S. V., de Araujo Oliveira, V., et al., 2023, Effectiveness of mRNA Boosters After Homologous Primary Series With BNT162b2 or ChAdOx1 Against Symptomatic Infection and Severe COVID-19 in Brazil and Scotland: A Test-Negative Design Case–Control Study. PLoS Med, 20(1):1–15.

[13]. Sapkota, B., Saud, B., Shrestha, R., Al-fahad, D., Sah, R., Shrestha, S., et al., 2022, Heterologous Prime – Boost Strategies for COVID-19 Vaccines, 1–10.

[14]. Bert, F., Scaioli, G., Vola, L., Accortanzo, D., Lo Moro, G., Siliquini, R., 2022, Booster Doses of Anti COVID-19 Vaccines: An Overview of Implementation Policies Among OECD and EU Countries. Int J Environ Res Public Health, 19(12).

[15]. Limbu, Y. B., Huhmann, B. A., 2023, Why Some People are Hesitant to Receive COVID-19 Boosters: A Systematic Review. Trop Med Infect Dis, 8(3):159.

[16]. Galanis, P., Vraka, I., Katsiroumpa, A., Siskou, O., Konstantakopoulou, O., Katsoulas, T., et al., 2022, Predictors of Willingness of the General Public to Receive a Second COVID-19 Booster Dose or a New COVID-19 Vaccine: A Cross-Sectional Study in Greece. Vaccines, 10(7):1–12.

[17]. Meng, H., Mao, J., Ye, Q., 2022, Strategies and Safety Considerations of Booster Vaccination in COVID-19. Bosn J Basic Med Sci, 22(3):366–73.

[18]. Wong, M. T. J., Dhaliwal, S. S., Balakrishnan, V., Nordin, F., Norazmi, M. N., Tye, G. J., 2023, Effectiveness of Booster Vaccinations on the Control of COVID-19 During the Spread of Omicron Variant in Malaysia. Int J Environ Res Public Health, 20(2).

[19]. Poh, X. Y., Lee, I. R., Lim, C., Teo, J., Rao, S., Chia, P. Y., et al., 2022, Evaluation of the Safety and Immunogenicity of Different Covid-19 Vaccine Combinations in Healthy Individuals: Study Protocol for a Randomized, Subject-Blinded, Controlled Phase 3 Trial [PRIBIVAC]. Trials, 23(1):1–13, https://doi.org/10.1186/s13063-022-06345-2

[20]. Atyeo, C., Shook, L. L., Nziza, N., Deriso, E. A., Muir, C., Baez, A. M., et al., 2023, COVID-19 Booster Dose Induces Robust Antibody Response in Pregnant, Lactating, and Nonpregnant Women. Am J Obstet Gynecol, 228(1):68.e1-68.e12, https://doi.org/10.1016/j.ajog.2022.07.014

[21]. Badell, M. L., Dude, C. M., Rasmussen, S. A., Jamieson, D. J., 2022, Covid-19 Vaccination in Pregnancy. BMJ, 11.

[22]. Ryalat, S., Alduraidi, H., Al-Ryalat, S. A., Alzu’bi, M., Alzyoud, M., Odeh, N., et al., 2022, Attitudes Towards COVID-19 Booster Vaccines, Vaccine Preferences, Child Immunization, and Recent Issues in Vaccination among University Students in Jordan. Vaccines, 10(8).

[23]. Verplanken, B., Orbell, S., 2022, Attitudes Habits and Behavior Change.

[24]. Cragg, L., 2016, The Development of Stimulus and Response Interference Control in Midchildhood. Dev Psychol, 52(2):242–52.

[25]. Tharwat, S., Eleraky, E. S., Adel, T., Nassar, M. K., Saleh, M., 2023, Attitudes and Concerns Regarding Booster Dose of Covid-19 Vaccine Among Egyptian Patients with Autoimmune and Rheumatic Diseases: A Cross-Sectional Survey Study. J Pharm Policy Pract, 16(1):1–12, https://doi.org/10.1186/s40545-023-00558-9

[26]. De Giorgio, A., Kuvačić, G., Maleš, D., Vecchio, I., Tornali, C., Ishac, W., et al., 2022, Willingness to Receive COVID-19 Booster Vaccine: Associations Between Green-Pass, Social Media Information, Anti-Vax Beliefs, and Emotional Balance. Vaccines, 10(3):1–18.

[27]. Shah, A., Coiado, O. C., 2023, COVID-19 Vaccine and Booster Hesitation Around the World: A Literature Review. Front Med, 9.

[28]. Elrod, J. K., Fortenberry, J. L., 2020, Advertising in Health and Medicine: Using Mass Media to Communicate With Patients. BMC Health Serv Res, 20(Suppl 1):1–8, https://dx.doi.org/10.1186/s12913-020-05599-3

[29]. Piekos, S., Hwang, Y. M., Roper, R., Sorensen, T., Price, N., Hood, L., et al., 2022, The Effect of COVID-19 Vaccination and Booster on Maternal-Fetal Outcomes. SSRN Electron J.

[30]. Osuagwu, U. L., Mashige, K. P., Ovenseri-Ogbomo, G., Envuladu, E. A., Abu, E. K., Miner, C. A., et al., 2023, The Impact of Information Sources on Covid-19 Vaccine Hesitancy and Resistance in Sub-Saharan Africa. BMC Public Health, 23(1):1–16, https://doi.org/10.1186/s12889-022-14972-2

[31]. Okorie, N., 2022, Global Media Framing, COVID-19 and the Issue of Vaccination: An Empirical Inquisition. Heal Promot Perspect, 12(2):186–191.

[32]. Wang, Y., Dai, Y., Li, H., Song, L., 2021, Social Media and Attitude Change: Information Booming Promote or Resist Persuasion? Front Psychol, 12(6):1–9.

[33]. Kreslake, J. M., Elkins, A., Thomas, C. N., Gates, S., Lehman, T., 2019, Use of Mass Communication by Public Health Programs in Nonmetropolitan Regions. Prev Chronic Dis, 16(7):1–6.

[34]. Kanchan, S., Gaidhane, A., 2023, Social Media Role and its Impact on Public Health: A Narrative Review. Cureus, 15(1):1–10.

[35]. Stellefson, M., Paige, S. R., Chaney, B. H., Chaney, J. D., 2020, Evolving Role of Social Media in Health Promotion: Updated Responsibilities for Health Education Specialists. Int J Environ Res Public Health, 17(4).

[36]. Joseph, A. M., Fernandez, V., Kritzman, S., Eaddy, I., Cook, O. M., Lambros, S., et al., 2022, COVID-19 Misinformation on Social Media: A Scoping Review. Cureus, 14(4):6–15.

[37]. Ahmad Rizal, A. R., Nordin, S. M., Ahmad, W. F. W., Ahmad Khiri, M. J., Hussin, S. H., 2022, How Does Social Media Influence People to Get Vaccinated? The Elaboration Likelihood Model of a Person’s Attitude and Intention to Get COVID-19 Vaccines. Int J Environ Res Public Health, 9(4).

[38]. Bardus, M., Assaf, S. A., Sakr, C. J., 2023, Using Social Marketing to Promote COVID-19 Vaccination Uptake: A Case Study from the “AUBe Vaccinated” Campaign. Vaccines, 11(2):1–14.

[39]. Ahiakpa, J. K., Cosmas, N. T., Anyiam, F. E., Enalume, K. O., Lawan, I., Gabriel, I. B., et al., 2022, COVID-19 Vaccines Uptake: Public knowledge, Awareness, Perception and Acceptance Among Adult Africans. PLoS One, 17(6):1–21, https://dx.doi.org/10.1371/journal.pone.0268230

[40]. Su, L., Du, J., Du, Z., 2022, Government Communication, Perceptions of COVID-19, and Vaccination Intention: A Multi-Group Comparison in China. Front Psychol, 12(1):1–11.

[41]. Elwy, A. R., Maguire, E. M., Kim, B., West, G. S., 2022, Involving Stakeholders as Communication Partners in Research Dissemination Efforts. J Gen Intern Med, 37:123–7.

[42]. Hudson, A., Montelpare, W. J., 2021, Predictors of Vaccine Hesitancy: Implications for Covid-19 Public Health Messaging. Int J Environ Res Public Health, 18(15).

[43]. Rao, R., Koehler, A., Beckett, K., Sengupta, S., 2022, COVID-19 Vaccine Mandates for Healthcare Professionals in the United States. Vaccines, 10(9):1–7.

[44]. Andrade, G., Bedewy, D., Bani, I., 2022, Motivational Factors to Receive the COVID-19 Vaccine in the United Arab Emirates: A Cross-Sectional Study. J Community Health, 47(3):530–8, https://doi.org/10.1007/s10900-022-01084-6

[45]. Soni, G. K., Bhatnagar, A., Gupta, A., Kumari, A., Arora, S., Seth, S., et al., 2023, Engaging Faith-Based Organizations for Promoting the Uptake of COVID-19 Vaccine in India: A Case Study of a Multi-Faith Society. Vaccines, 11(4).

[46]. Elmore, C. E., Blackstone, S. R., Carpenter, E. L., de Cortez, P. I., O’donnell, C., Uhlmann, E., et al., 2022, Advancing COVID-19 Vaccination Equity Among the Refugee Community: An Innovative Multi-Sector Collaborative Outreach Program. J Health Care Poor Underserved, 33(2):25–43.

Abstract:

Child mortality is a major concern in Africa, particularly due to diarrhoea and respiratory tract infections, which are the leading causes. These diseases are often transmitted through poor handwashing practices. This study aimed to examine the factors that influence handwashing practices, as well as the knowledge, awareness, and actual practices of handwashing among primary school students in the Karongi district, Rwanda. A mixed methods approach was utilized, combining quantitative data gathered through an online questionnaire with qualitative data obtained from semi-structured interviews. Interviews were conducted with teachers and students to gain insight into the existing hygiene practices and support systems in place. The sample size consisted of 583 students and 120 teachers. Quantitative data was analyzed using SPSS version 25.0, incorporating Fisher's exact, Chi-square, correlation, and multiple regression tests. Qualitative data was analyzed using NVivo version 14.0. The correlation (r=0.75; p<0.001) and multiple regression analyses demonstrated that knowledge of handwashing practices significantly influenced awareness (r=0.612; p<0.001), the availability of hygiene facilities (r=0.665; p<0.001), and the implementation of hygiene practices. The students and teachers in Karongi possess a certain level of knowledge regarding handwashing techniques. Teachers in all primary schools supported the practice of handwashing. Students were washing their hands with soap and water as a means of reducing the transmission of diseases. However, more emphasis on instilling proper handwashing practices is required. Handwashing campaigns should be utilized to encourage good hygiene practices among children, as the primary schools already have adequate facilities in place.

References:

Abstract:

The conventional methods of drug delivery, primarily oral and injectable routes, have encountered limitations in effectively administering new drugs. These limitations arise particularly with drugs such as proteins and nucleic acids, where traditional routes may not be optimal for therapeutic efficacy. Novel biological medications necessitate innovative delivery strategies to mitigate adverse effects and enhance patient compliance. Nanometer-sized drug particles present unique characteristics that offer potential improvements in various dosage formats. Within this size range, particles exhibit resistance to settling, increased saturation solubility, rapid dissolution, and enhanced adherence to biological surfaces. Consequently, these properties facilitate a quicker onset of therapeutic action and improved bioavailability. The emergence of nanotechnology provides scientists with a versatile tool to address both traditional and innovative drug delivery challenges. By leveraging nanotechnology, researchers explore new avenues for drug delivery, striving to optimize therapeutic outcomes while minimizing adverse effects. This review underscores the transformative potential of nano-drug delivery systems in advancing pharmaceutical sciences and enhancing patient care.

References:

Abstract:

Microbiota in the intestine produces SCFAs like Butyrate and Propionate through undigested fiber, which plays an important role in metabolic disorders, especially in obesity and type II diabetes. To compare the anti-obesity activity of probiotics with SCFAs through GLP-1 and PYY activity in HFD induced obesity in rats. For the study, rats weighing around 150-200g were chosen, separated into multiple groups, and given Fat-enriched diet for 12 weeks to trigger obesity. SCFAs like Butyrate, propionate (400mg/kg, P.O), and probiotics (10⁸ CFU P.O) were continuously administered to their groups along with the HFD. Weekly once the animal's BW & daily food intake by the animal, and every 15 days once lipid profile, GLP, PYY, insulin, and leptin parameters were measured. After the completion of the study blood was collected through retro-orbital puncture for evaluation of biochemical parameters and animals were anesthetized and sacrificed for organ isolation for histopathological studies. Administration of SCFAs along with Probiotics in HFD-induced Overweight rats led to a notable drop in BW, food intake, fat accumulation, fasting insulin, leptin, and lipids throughout 12 weeks as compared with rats fed with HFD and standard. SCFAs along with probiotics effectively increase HDL levels and reduce LDL. Histopathology examination reveals that fat tissue accumulation was absent in treated groups when compared with only the HFD feed group. Results show that SCFAs are showing a notable decrease in lipid profile, food intake, and body weight than probiotics-treated group possesses significant anti-obesity activity.

References:

[1].   Bhutani, K. K., Birari, R., Kapat, K., 2007, Potential Anti-Obesity and Lipid Lowering Natural Products: A Review. Natural Product Communications,2(3). https://doi:10.1177/1934578X0700200316

[2].   Mopuri, R., Ganjayi, M., Banavathy, K. S., Parim, B. N., Meriga, B., 2015, Evaluation of Anti-Obesity Activities of Ethanolic Extract of Terminalia Paniculata Bark on High Fat Diet-Induced Obese Rats. BMC Complement Altern Med. Mar 24,15,76. https://doi:10.1186/s12906-015-0598-3

[3].   Yuan, H. -D., Kim, S. -J.; Quan, H.-Y., Huang, B., Chung, S. -H., 2010, Ginseng Leaf Extract Prevents High Fat Diet-Induced Hyperglycemia and Hyperlipidemia Through AMPK Activation. J. Ginseng Res. 34, 369–375. https://doi.org/10.5142/jgr.2010.34.4.369

[4].   Saravanan, G., Ponmurugan, P., Deepa, M. A., Senthilkumar, B., 2014, Anti-Obesity Action of Gingerol: Effect on Lipid Profile, Insulin, Leptin, Amylase and Lipase in Male Obese Rats Induced by a High-Fat Diet. J Sci Food Agric, Nov, 94(14), 2972-7. https://doi:10.1002/jsfa.6642

[5].   Jura, M., Kozak, L. P., 2016, Obesity and Related Consequences to Ageing, Age (Dordr). Feb;38(1):23. https://doi:10.1007/s11357-016-9884-3

[6].   Dietz, W. H., Baur, L. A., Hall, K., Puhl, R. M., Taveras, E. M., Uauy, R., Kopelman, P., 2015 Management of Obesity: Improvement of Health-Care Training and Systems for Prevention and Care. Lancet. Jun 20;385(9986):2521-33. https://doi:10.1016/S0140-6736(14)61748-7

[7].   Kaczmarczyk, M. M., Miller, M. J., 2012, The Health Benefits of Dietary Fiber: Beyond the Usual Suspects of Type 2 Diabetes Mellitus, Cardiovascular Disease and Colon Cancer. Metabolism: Clinical and experimental, 61(8), 1058–1066. https://doi.org/10.1016/j.metabol.2012.01.017

[8].   Edwards, C. A., Xie, C., Garcia, A. L., 2015, Dietary Fibre and Health in Children and Adolescents. Proc Nutr Soc. Aug;74(3):292-302. https://doi:10.1017/S0029665115002335

[9].   Liu, X., Wu, Y., Li, F., Zhang, D., 2015, Dietary Fiber Intake Reduces Risk of Inflammatory Bowel Disease: Result from a Meta-Analysis. Nutr Res. Sep,35(9),753-8. https://doi:10.1016/j.nutres.2015.05.021

[10]. Keenan, M. J, Zhou, J., Hegsted, M., Pelkman, C., Durham, H. A., Coulon, D. B., Martin, R. J., 2015 Role of Resistant Starch in Improving Gut Health, Adiposity, and Insulin Resistance. Adv Nutr, 6(2),198-205. https://doi:10.3945/an.114.007419

[11]. Anastasovska, J., Arora, T., et al. 2012, Fermentable carbohydrate Alters Hypothalamic Neuronal Activity and Protects Against the Obesogenic Environment, 20(5), 1016–1023. https://doi.org/10.1038/oby.2012.6

[12]. Wanders, A. J., van den Borne, J. J., et.al, 2011, Effects of Dietary Fibre on Subjective Appetite, Energy Intake and Body Weight: A Systematic Review of Randomized Controlled Trials, 12(9), 724–739. https://doi.org/10.1111/j.1467-789X.2011.00895.x

[13]. Tan, J., McKenzie, C., Potamitis, M., et.al. 2014, The Role of Short-Chain Fatty Acids in Health and Disease. Advances in Immunology, 121, 91–119. https://doi.org/10.1016/B978-0-12-800100-4.00003-9

[14]. Canfora, E. E., Jocken, J. W., & Blaak, E. E., 2015, Short-Chain Fatty Acids in Control of Bodyweight and Insulinsensitivity,11(10),577591.https://doi.org/10.1038/nrendo.2015.128

[15]. Tazoe, H., Otomo, Y., Karaki, S., Kato, I., Fukami, Y., Terasaki, M., & Kuwahara, A. 2009, Expression of Short-Chain Fatty Acid Receptor GPR41 in the Human Colon. Biomedical Research, 30(3), 149–156. https://doi.org/10.2220/biomedres.30.149

[16]. Samuel, B. S., Shaito, A., Motoike, T., et.al 2008, Effects of the Gut Microbiota on Host Adiposity are Modulated by the Short-Chain Fatty-Acid Binding G Protein-Coupled Receptor, Gpr41, 105(43), 16767–16772. https://doi.org/10.1073/pnas.0808567105

[17]. Nemet, I., et al. 2020, A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts Via Adrenergic Receptors.Cell,180(5),862–877.e22.https://doi.org/10.1016/j.cell.2020.02.016

[18]. Chaudhary, P. K., & Kim, S., 2021, An Insight into GPCR and G-Proteins as Cancer Drivers. Cells, 10(12), 3288. https://doi.org/10.3390/cells10123288

[19]. OECD 2008, Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris, https://doi.org/10.1787/9789264070684-en

[20]. Luna, L. G., 1968, Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd Edition, McGraw-Hill, New York.p. 258.

[21]. Kim, A., -Young & Jeong, et.al 2012, Dose Dependent Effects of Lycopene Enriched Tomato-Wine on Liver and Adipose Tissue in High-Fat Diet Fed Rats, 130. 42-48. https://doi.org/10.1016/j.foodchem.2011.06.050

[22]. BrahmaNaidu, P., et.al 2014, Mitigating Efficacy of Piperine in the Physiological Derangements of High Fat Diet Induced Obesity in Sprague Dawley Rats, 221, 42–51. https://doi.org/10.1016/j.cbi.2014.07.008

[23]. Shah, S. S., et.al 2011, Effect of Piperine in the Regulation of Obesity-Induced Dyslipidemia in High-Fat Diet Rats. Indian Journal of Pharmacology, 43(3), 296–299. https://doi.org/10.4103/0253-7613.81516

[24]. Johnson, R., McNutt, P., MacMahon, S., & Robson, R., 1997, Use of the Friedewald Formula to Estimate LDL-Cholesterol in Patients with Chronic Renal Failure on Dialysis. Clinical Chemistry, 43(11), 2183–2184.

[25]. Muruganandan, S., Srinivasan, K., Gupta, S., Gupta, P. K., & Lal, J., 2005, Effect of Mangiferin on Hyperglycemia and Atherogenicity in Streptozotocin Diabetic Rats, 97(3), 497–501. https://doi.org/10.1016/j.jep.2004.12.010

[26]. Suanarunsawat, T., et.al 2010, Antioxidant Activity and Lipid-Lowering Effect of Essential Oils Extracted from Ocimum Sanctum L. Leaves in Rats Fed with a High Cholesterol Diet. Journal of Clinical Biochemistry and Nutrition, 46(1), 52–59. https://doi.org/10.3164/jcbn.09-52

[27]. Kimura, I., et.al 2011, Short-Chain Fatty Acids and Ketones Directly Regulate Sympathetic Nervous System Via G Protein-Coupled Receptor 41 (GPR41), 108(19), 8030–8035. https://doi.org/10.1073/pnas.1016088108

Abstract:

Child mortality in Africa is a pressing issue, particularly due to diarrhea and respiratory tract infections. These diseases are often transmitted through improper handwashing practices. This research aimed to identify the factors that influence handwashing habits and assess the availability of adequate facilities and water sources in primary schools in the Karongi district. A mixed-method approach was used to collect data from 583 students through structured questionnaires and 120 teachers through semi-structured interviews. Additionally, interviews with parents and community members were conducted to gain broader insights into community hygiene practices and support systems. Quantitative analysis using SPSS showed that promoting handwashing among school children reduces illness and absenteeism. Over two-thirds (67.2%) of students reported receiving effective support from their teachers, and another two-thirds (66.7%) stated that they wash their hands with soap and water. Moreover, close to another two-thirds (64%) of students acknowledged the importance of hygiene in disease prevention, and nearly two-thirds (60.9%) were aware of the health risks associated with poor hygiene. Qualitative findings revealed that while primary schools have sufficient hygiene facilities, there are challenges such as water shortages, inadequate hygiene resources, a limited number of washing stations, and water supply shortages. The study concludes that it is crucial to promote handwashing through educational campaigns to reduce disease transmission and improve the health outcomes of school children in Rwanda. Additionally, it emphasizes the need to address resource challenges in primary schools.

References:

[1]. Gawai, P. P., Taware, S. A., Chatterjee, A. S., & Thakur, H. P., 2016, A Cross Sectional Descriptive Study of Hand Washing Knowledge and Practices Among Primary School Children in Mumbai, Maharashtra, India. Int J Community Med Public Health, 3(10), 2958-2966, https://doi.org/10.18203/2394-6040.ijcmph20163391

[2]. Khatoon, R., Sachan, B., Khan, M. A., & Srivastava, J. P., 2017, Impact of School Health Education Program on Personal Hygiene Among School Children of Lucknow District. Journal of Family Medicine and Primary Care, 6(1), 97-100, https://doi.org/10.4103/2249-4863.214973

[3]. Bloomfield, S. F., Aiello, A. E., Cookson, B., O'Boyle, C., & Larson, E. L., 2007, The Effectiveness of Hand Hygiene Procedures in Reducing the Risks of Infections in Home and Community Settings Including Handwashing and Alcohol-Based Hand Sanitizers. American Journal of Infection Control, 35(10), S27-S64, https://doi.org/10.1016/j.ajic.2007.07.001

[4]. McMichael, C., 2019, Water, Sanitation and Hygiene (Wash) in Schools in Low-Income Countries: A Review of Evidence of Impact. International Journal of Environmental Research and Public Health,16(3), 359, https://doi.org/10.3390/ijerph16030359

[5]. Setyautamii, T., Sermsri, S., & Chompikul, J., 2012, Proper Hand Washing Practices Among Elementary School Students in Selat Sub-District, Indonesia. Journal of Public Health and Development, 10 (2), 3–20, Retrieved From https://he01.tci-thaijo.org/index.php/AIHD-MU/article/view/2642

[6]. Admasie, A., Guluma, A., & Feleke, F. W., 2022, Handwashing Practices and its Predictors Among Primary School Children in Damote Woide District, South Ethiopia: An Institution Based Cross-Sectional Study. Environmental Health Insights, 16, https://doi.org/10.1177/11786302221086795

[7]. World health organization (WHO) 2015, Clean Care is Safer Care, Retrieved From https://www.who.int/gpsc/clean_hands_protection/en/

[8]. Biswas, D., Sahoo, S., Dasgupta, A., Preeti, P. S., & Amitavakumar Das, S., 2015, Quantification of Perception Status of Hand Washing Practice Among School Children in a Rural Area of West Bengal. Scholars Journal of Applied Medical Sciences, 3(4), 1683-1687, https://doi.org/10.36347/sjams.2015.v03i04.015

[9]. Mane, A. B., Reddy, N. S., Reddy, P., Chetana, K. V., Srijith, S. N., & Sriniwas, T., 2016, Differences of Hand Hygiene and its Correlates Among School Going Children in Rural and Urban Area of Karnataka, India. Archives of Medicine, 8(5), 1-5, https://doi.org/10.21767/1989-5216.1000163

[10]. Anand, D., & Prakash, S., 2018, Assessment of the Hygiene and Sanitation Practices of Students of Class VI to IX in Urban Government Inter College at Allahabad District, India. Int J Community Med Public Health, 5(9), 3870-3875, https://doi.org/10.18203/2394-6040.ijcmph20183428

[11]. Louis, E. F., Eugene, D., Ingabire, W. C., Isano, S., & Blanc, J., 2022, Rwanda's Resiliency During the Coronavirus Disease Pandemic. Frontiers in Psychiatry, 12, https://doi.org/10.3389/fpsyt.2021.589526

[12]. Steiner-Asiedu, M., Van-Ess, S. E., Papoe, M., Setorglo, J., Asiedu, D. K., & Anderson, A. K., 2011, Hand Washing Practices Among School Children in Ghana. Current Research Journal of Social Sciences,3(4), 293-300, Retrieved From https://maxwellsci.com/print/crjss/v3-293-300.pdf

[13]. Nwajiuba, C. A., Ogunji, C. V., Uwakwe, R. C., & David, E. I., 2019 Handwashing Practices Among Children in Public Schools in Imo State, Nigeria. Global Journal of Health Science, 11(14), 15, https://doi.org/10.5539/gjhs.v11n14p15

[14]. Almoslem, M. M., Alshehri, T. A., Althumairi, A. A., Aljassim, M. T., Hassan, M. E., & Berekaa, M. M., 2021, Handwashing Knowledge, Attitudes, and Practices Among Students in Eastern Province Schools, Saudi Arabia. Journal of Environmental and Public Health, https://doi.org/10.1155/2021/6638443/

[15]. Habumugisha, V., 2018, Assessing Students’ Knowledge, Attitudes and Practices on Water, Sanitation, Hygiene, and Related Diseases in Selected Schools in Musanze District, Rwanda (Master's thesis), Retrieved From https://repository.pauwes cop.net/bitstream/handle/1/263/MT_Vincent%20HABUMUGISHA.pdf?sequence=1&isAllowed=y

[16]. Bangdiwala, S. I., 2019, Basic Epidemiology Research Designs I: Cross-Sectional Design. International Journal of Injury Control and Safety Promotion, 26(1), 124-126, https://doi.org/10.1080/17457300.2018.1556415

[17]. Cuemath, (n.d.), Sample Size Formula-What is Sample Size Formula? Examples, Retrieved From https://www.cuemath.com/sample-size-formula/

[18]. Berhanu, A., Mengistu, D. A., Temesgen, L. M., Mulat, S., Dirirsa, G., Alemu, F. K., ... & Geremew, A., 2022, Hand Washing Practice Among Public Primary School Children and Associated Factors in Harar town, eastern Ethiopia: An Institution-Based Cross-Sectional Study. Frontiers in Public Health, 10, 975507, https://doi.org/10.3389/fpubh.2022.975507/

[19]. Twinomuhwezi, B., 2021, Factors Associated with Hand Washing Practices Among Secondary School Students in Ndorwa East Constituency in Kabale District (Doctoral Dissertation, Kabale University), Retrieved From https://backend.kab.ac.ug/server/api/core/bitstreams/d434141d-4e54-4d9b-81a5-4cf08df38479/content

[20]. Chen, X., Ran, L., Liu, Q., Hu, Q., Du, X., & Tan, X., 2020, Hand Hygiene, Mask-Wearing Behaviors and its Associated Factors During the COVID-19 Epidemic: A Cross-Sectional Study Among Primary School Students in Wuhan, China. International Journal of Environmental Research and Public Health, 17(8), 2893, https://doi.org/10.3390/ijerph17082893

[21]. Contzen, N., De Pasquale, S., & Mosler, H. J., 2015, Over-Reporting in Handwashing Self-Reports: Potential Explanatory Factors and Alternative Measurements. PloS one,10(8), e0136445, https://doi.org/10.1371/journal.pone.0136445/

[22]. Rosenman, R., Tennekoon, V., & Hill, L. G., 2011, Measuring Bias in Self-Reported Data. International Journal of Behavioural and Healthcare Research, 2(4), 320-332, https://doi.org/10.1504/IJBHR.2011.043414/

Abstract:

This study explores the green synthesis of cobalt nanoparticles (CoNPs) using Butea monosperma flower extract, highlighting their antibacterial efficacy, toxicity, and biocompatibility. Utilizing a plant-based reduction method, cobalt ions were reduced and stabilized by the bioactive compounds in the flower extract, forming CoNPs with notable uniformity and stability. Characterization techniques, including, UV-VIS spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDAX), confirmed the formation and nature of the nanoparticles, which exhibited an average size of 10-20 nm. The synthesized CoNPs demonstrated significant antibacterial activity against both Gram-positive and Gram-negative bacteria, suggesting their potential as effective antimicrobial agents. Toxicity assessment using zebrafish, and brine shrimp lethality assay (BSLA), revealed that the nanoparticles exhibited minimal toxicity. Biocompatibility studies further indicated that CoNPs had no adverse effects on cellular morphology or proliferation, highlighting their suitability for biomedical applications. The eco-friendly synthesis method not only provides a sustainable approach to nanoparticle production but also enhances the potential for their safe application in medical and environmental fields. The findings underscore the promise of B. monosperma-mediated CoNPs as a viable alternative to chemically synthesized nanoparticles, with significant implications for antibacterial therapies and biocompatible materials.

References:

[1].   Vodyashkin, A. A., Kezimana, P., Prokonov, F. Y., Vasilenko, I. A., and Stanishevskiy, Y. M., 2022. Current Methods for Synthesis and Potential Applications of Cobalt Nanoparticles: A review. Crystals, 12(2),272. https://doi.org/10.3390/cryst12020272

[2].   Alsaiari, N. S., Alzahrani, F. M., Amari, A., Osman, H., Harharah, H. N., Elboughdiri, N., and Tahoon, M. A., 2023. Plant and Microbial Approaches as Green Methods for the Synthesis of Nanomaterials: Synthesis, Applications, and Future Perspectives. Molecules, 28(1), 463. https://doi.org/10.3390/molecules28010463

[3].   Srivathsan, J., Sivakami, V., Ramachandran, B., Harikrishna, K.S., Vetriselvi, S. and Kumar, D.J.M., 2012. Synthesis of silver nanoparticles and its effect on soil bacteria. J. Microbiol. Biotech. Res, 2(6), pp.871-874.

[4].   Khusnuriyalova, A. F., Caporali, M., Hey‐Hawkins, E., Sinyashin, O. G., and Yakhvarov, D. G., 2021. Preparation of Cobalt Nanoparticles. European Journal of Inorganic Chemistry, 2021(30), 3023-3047. https://doi.org/10.1002/ejic.202100367

[5].   Pusta, A., Tertis, M., Crăciunescu, I., Turcu, R., Mirel, S., and Cristea, C., 2023. Recent Advances in the Development of Drug Delivery Applications of Magnetic Nanomaterials. Pharmaceutics, 15(7), 1872. https://doi.org/10.3390/pharmaceutics15071872

[6].   Farzin, A., Etesami, S. A., Quint, J., Memic, A., and Tamayol, A., 2020. Magnetic Nanoparticles in Cancer Therapy and Diagnosis. Advanced Healthcare Materials, 9(9), 1901058. https://doi.org/10.1002/adhm.201901058

[7].   Ryntathiang, I., Behera, A., Richard, T., and Jothinathan, M. K. D., 2024. An Assessment of the in Vitro Antioxidant Activity of Cobalt Nanoparticles Synthesized from Millettia Pinnata, Butea Monosperma, and Madhuca Indica Extracts: A Comparative Study. Cureus, 16(4), e59112. https://doi.org/10.7759/cureus.59112

[8].   Abass, A. A., Alaarage, W. K., Abdulrudha, N. H., and Haider, J., 2021. Evaluating the Antibacterial Effect of Cobalt Nanoparticles Against Multi-Drug Resistant Pathogens. Journal of Medicine and Life, 14(6), 823. https://doi.org/10.25122/jml-2021-0270

[9].   Kharade Suvarta, D., Nikam Gurunath, H., Mane Gavade Shubhangi, J., Patil Sachinkumar, R., and Gaikwad Kishor, V., 2020. Biogenic Synthesis of Cobalt Nanoparticles using Hibiscus Cannabinus Leaf Extract and their Antibacterial Activity. Research Journal of Chemistry and Environment, 24(5), 9-13. https://www.researchgate.net/profile/Sachinkumar-Patil/publication/340844918

[10].           Cruz, J. C., Nascimento, M. A., Amaral, H. A., Lima, D. S., Teixeira, A. P. C., and Lopes, R. P., 2019. Synthesis and Characterization of Cobalt Nanoparticles for Application in the Removal of Textile Dye. Journal of Environmental Management, 242, 220-228. https://doi.org/10.1016/j.jenvman.2019.04.059

[11].   Fardood, S. T., Forootan, R., Moradnia, F., Afshari, Z., and Ramazani, A., 2020. Green Synthesis, Characterization, and Photocatalytic Activity of Cobalt Chromite Spinel Nanoparticles. Materials Research Express, 7(1), p.015086. https://doi.org/10.1088/2053-1591/ab6c8d

[12].   Kus-Liśkiewicz, M., Fickers, P., and Ben Tahar, I., 2021. Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations. International Journal of Molecular Sciences, 22(20), 10952. https://doi.org/10.3390/ijms222010952

[13].  Ansari, S. M., Bhor, R. D., Pai, K. R., Sen, D., Mazumder, S., Ghosh, K., Kolekar, Y. D., and Ramana, C.V., 2017. Cobalt Nanoparticles for Biomedical Applications: Facile Synthesis, Physiochemical Characterization, Cytotoxicity Behavior and Biocompatibility. Applied Surface Science, 414, 171-187. https://doi.org/10.1016/j.apsusc.2017.03.002

[14].  Bittner, M., Štern, A., Smutná, M., Hilscherová, K., and Žegura, B., 2021. Cytotoxic and Genotoxic Effects of Cyanobacterial and Algal Extracts—Microcystin and Retinoic Acid Content. Toxins, 13(2), 107. https://doi.org/10.3390/toxins13020107

[15].  Zhao, W., Chen, Y., Hu, N., Long, D., and Cao, Y., 2024. The Uses of Zebrafish (Danio rerio) as an in Vivo Model for Toxicological Studies: A Review Based on Bibliometrics. Ecotoxicology and Environmental Safety, 272, 116023. https://doi.org/10.1016/j.ecoenv.2024.116023

[16].  Modarresi Chahardehi, A., Arsad, H., and Lim, V., 2020. Zebrafish as a Successful Animal Model for Screening Toxicity of Medicinal Plants. Plants, 9(10),1345. https://doi.org/10.3390/plants9101345

[17].  Haque, E., and Ward, A. C., 2018. Zebrafish as a Model to Evaluate Nanoparticle Toxicity. Nanomaterials, 8(7), 561. https://doi.org/10.3390/nano8070561

[18].  Miyawaki, I., 2020. Application of Zebrafish to Safety Evaluation in Drug Discovery. Journal of Toxicologic Pathology, 33(4), 197-210. https://doi.org/10.1293/tox.2020-0021

[19].  Payne-Sturges, D. C., Scammell, M. K., Levy, J. I., Cory-Slechta, D. A., Symanski, E., Carr Shmool, J. L., Laumbach, R., Linder, S., and Clougherty, J. E., 2018. Methods for Evaluating the Combined Effects of Chemical and Nonchemical Exposures for Cumulative Environmental Health Risk Assessment. International Journal of Environmental Research and Public Health, 15(12), 2797. https://doi.org/10.3390/ijerph15122797

[20].  Cassar, S., Adatto, I., Freeman, J. L., Gamse, J. T., Iturria, I., Lawrence, C., Muriana, A., Peterson, R. T., Van Cruchten, S., and Zon, L. I., 2019. Use of Zebrafish in Drug Discovery Toxicology. Chemical Research in Toxicology, 33(1), 95-118. https://doi.org/10.1021/acs.chemrestox.9b00335

[21].  Frisch, E., Clavier, L., Belhamdi, A., Vrana, N. E., Lavalle, P., Frisch, B., Heurtault, B., and Gribova, V., 2023. Preclinical in Vitro Evaluation of Implantable Materials: Conventional Approaches, New Models and Future Directions. Frontiers in Bioengineering and Biotechnology, 11, 1193204. https://doi.org/10.3389/fbioe.2023.1193204

[22].  Othman, Z., Pastor, B. C., van Rijt, S., and Habibovic, P., 2018. Understanding Interactions Between Biomaterials and Biological Systems Using Proteomics. Biomaterials, 167, 191-204. https://doi.org/10.1016/j.biomaterials.2018.03.020

[23].  Egbuna, C., Parmar, V. K., Jeevanandam, J., Ezzat, S. M., Patrick-Iwuanyanwu, K. C., Adetunji, C. O., Khan, J., Onyeike, E. N., Uche, C. Z., Akram, M., and Ibrahim, M. S., 2021. Toxicity of Nanoparticles in Biomedical Application: Nanotoxicology. Journal of Toxicology, 2021(1), 9954443. https://doi.org/10.1155/2021/9954443

[24].  Witika, B. A., Makoni, P. A., Matafwali, S. K., Chabalenge, B., Mwila, C., Kalungia, A. C., Nkanga, C. I., Bapolisi, A. M. and Walker, R. B., 2020. Biocompatibility of Biomaterials for Nanoencapsulation: Current Approaches. Nanomaterials, 10(9),1649. https://doi.org/10.3390/nano10091649

[25].  Ryntathiang, I., Jothinathan, M. K. D., Behera, A., Saravanan, S., and Murugan, R., 2024. Comparative Bioactivity Analysis of Green-Synthesized Metal (Cobalt, Copper, and Selenium) Nanoparticles. Cureus, 16(3), e55933. https://doi.org/10.7759/cureus.55933

[26].  Munusamy, T., and Shanmugam, R., 2023. Green Synthesis of Copper Oxide Nanoparticles Synthesized by Terminalia chebula Dried Fruit Extract: Characterization and Antibacterial Action. Cureus, 15(12), e50142. https://doi.org/10.7759/cureus.50142

[27].  Anandan, J., and Shanmugam, R., 2024. Antioxidant, Anti-inflammatory, and Antimicrobial Activity of the Kalanchoe Pinnata and Piper Longum Formulation Against Oral Pathogens. Cureus, 16(4), e57824. https://doi.org/10.7759/cureus.57824

[28].  Rajeshkumar, S., Santhoshkumar, J., Vanaja, M., Sivaperumal, P., Ponnanikajamideen, M., Ali, D., and Arunachalam, K., 2022. Evaluation of Zebrafish Toxicology and Biomedical Potential of Aeromonas Hydrophila Mediated Copper Sulfide Nanoparticles. Oxidative Medicine and Cellular Longevity, 2022(1), 7969825. https://doi.org/10.1155/2022/7969825

[29].  Sankar, H. N., Shanmugam, R., and Anandan, J., 2024. Green Synthesis of Euphorbia Tirucalli-Mediated Titanium Dioxide Nanoparticles Against Wound Pathogens. Cureus, 16(2), e53939. https://doi.org/10.7759/cureus.53939

[30].  Ameena, M., Arumugham, M., Ramalingam, K., Rajeshkumar, S., and Perumal, E., 2023. Cytocompatibility and Wound Healing Activity of Chitosan Thiocolchicoside Lauric Acid Nanogel in Human Gingival Fibroblast Cells. Cureus, 15(8), e43727. https://doi.org/10.7759/cureus.43727

[31].  Ali, H., Dixit, S., and Alarifi, S., 2024. Biosynthesis and Screening of Cobalt Nanoparticles Using Citrus Species for Antimicrobial Activity. Open Chemistry, 22(1), 20240021. https://doi.org/10.1515/chem-2024-0021

[32].  Ali, S. G., Ansari, M. A., Alzohairy, M. A., Alomary, M. N., Jalal, M., AlYahya, S., Asiri, S. M. M. and Khan, H. M., 2020. Effect of Biosynthesized ZnO Nanoparticles on Multi-Drug Resistant Pseudomonas Aeruginosa. Antibiotics, 9(5), 260. https://doi.org/10.3390/antibiotics9050260

[33].  Kalanakoppal Venkatesh, Y., Mahadevaiah, R., Haraluru Shankaraiah, L., Ramappa, S., and Sannagoudar Basanagouda, A., 2018. Preparation of a CaO Nanocatalyst and its Application for Biodiesel Production Using Butea Monosperma Oil: An Optimization Study. Journal of the American Oil Chemists' Society, 95(5), 635-649. https://doi.org/10.1002/aocs.12079

[34].  Kumar, V., Harini, R., Anitha, G., and Nagaraju, G., 2024. Facile Green Synthesis of Zn Doped MoO3 Nanoparticles and its Photocatalytic and Photoluminescence Studies. Journal of Molecular Structure, 1312, 138494. https://doi.org/10.1016/j.molstruc.2024.138494

[35].  Mubraiz, N., Bano, A., Mahmood, T., and Khan, N., 2021. Microbial and Plant Assisted Synthesis of Cobalt Oxide Nanoparticles and their Antimicrobial Activities. Agronomy, 11(8), https://doi.org/1607.10.3390/agronomy11081607

[36].  Mmelesi, O. K., Masunga, N., Kuvarega, A., Nkambule, T.T., Mamba, B. B., and Kefeni, K. K., 2021. Cobalt Ferrite Nanoparticles and Nanocomposites: Photocatalytic, Antimicrobial Activity and Toxicity in Water Treatment. Materials Science in Semiconductor Processing, 123, 105523. https://doi.org/10.1016/j.mssp.2020.105523

[37].  Alyami, M. H., Fakhry, A. M., El Halfawy, N. M., Toto, S. M., Sedky, N. K., Yassin, H. A., Fahmy, S. A., and Mokhtar, F. A., 2023. Retama Monosperma Chemical Profile, Green Synthesis of Silver Nanoparticles, and Antimicrobial Potential: A Study Supported by Network Pharmacology and Molecular Docking. RSC Advances, 13(37), 26213-26228. https://doi.org/10.1039/D3RA05116A

[38].  Zafar, N., Madni, A., Khalid, A., Khan, T., Kousar, R., Naz, S. S. and Wahid, F., 2020. Pharmaceutical and Biomedical Applications of Green Synthesized Metal and Metal Oxide Nanoparticles. Current Pharmaceutical Design, 26(45), 5844-5865. https://doi.org/10.2174/1381612826666201126144805

[39].  Siddiqi, K. S., Rahman, A., Tajuddin, N., and Husen, A., 2018. Properties of Zinc Oxide Nanoparticles and their Activity Against Microbes. Nanoscale Research Letters, 13, 1-13. https://doi.org/10.1186/s11671-018-2532-3

[40].  d’Amora, M., Schmidt, T. J. N., Konstantinidou, S., Raffa, V., De Angelis, F., and Tantussi, F., 2022. Effects of Metal Oxide Nanoparticles in Zebrafish. Oxidative Medicine and Cellular Longevity, 2022(1), 3313016. https://doi.org/10.1155/2022/3313016

[41].  Chen, J., Lei, L., Mo, W., Dong, H., Li, J., Bai, C., Huang, K., Truong, L., Tanguay, R. L., Dong, Q., and Huang, C., 2021. Developmental Titanium Dioxide Nanoparticle Exposure Induces Oxidative Stress and Neurobehavioral Changes in Zebrafish. Aquatic Toxicology, 240, 105990. https://doi.org/10.1016/j.aquatox.2021.105990

[42].  Yan, S., Qian, Y., Haghayegh, M., Xia, Y., Yang, S., Cao, R., and Zhu, M., 2024. Electrospun Organic/Inorganic Hybrid Nanofibers for Accelerating Wound Healing: A Review. Journal of Materials Chemistry B, 12, 3171-3190. https://doi.org/10.1039/D4TB00149D

[43].  Chakraborty, C., Sharma, A. R., Sharma, G., and Lee, S. S., 2016. Zebrafish: A Complete Animal Model to Enumerate the Nanoparticle Toxicity. Journal of Nanobiotechnology, 14,1-13. https://doi.org/10.1186/s12951-016-0217-6

Abstract:

The development of functioning endometrial tissue in the abdominal wall after a cesarean birth is an uncommon case phenomenon known as scar endometriosis. Aside from the pelvis, endometriosis most often manifests in the bladder, gastrointestinal system, and, most notably, after obstetric surgical procedures. Here we present a case of caesarean scar endometriosis in a pregnant woman of 30 years of age. The patient has a history of type 1 diabetes mellitus, is now taking insulin and oral hypoglycaemic medications, and is being treated for hypothyroidism. She said that the region around her prior cesarean scar was swollen and painful periodically. USG showed ill defined heterogeneous hypo-echoic subcutaneous mass lesion approximately measuring 3*2*3cms over the right lateral one third of the caesarean scar site.MRI report showing a well-defined hypointense lesion in the anterior abdominal wall in the previous caesarean scar site suggestive of scar endometriosis. A total of three masses were removed, and scar endometriosis was verified by histology.

References:

[1].   Choudhury, H., Pandey, M., Hua, C. K., Mun, C. S., Jing, J. K., Kong, L., Ern, L. Y., Ashraf, N. A., Kit, S. W., Yee, T. S., Pichika, M. R., Gorain, B., & Kesharwani, P., 2018, An Update on Natural Compounds in the Remedy of Diabetes Mellitus: A Systematic Review. Journal of Traditional and Complementary Medicine, 8(3): 361–376. https://doi.org/10.1016/J.Jtcme.2017.08.012(2018)

[2].   Kharroubi, A. T., 2015, Diabetes Mellitus: The Epidemic of the Century. World Journal of Diabetes, 6(6): 850. https://doi.org/10.4239/Wjd.V6.I6.850

[3].   International Diabetes Federation, 2021, IDF Diabetes Atlas, 10th Edition. https://idf.org/About-Diabetes/Diabetes-Facts-Figures/(2021)

[4].   Missoun, F., 2018, Antidiabetic Bioactive Compounds from Plants. Medical Technologies Journal, 2(2): 199–214. https://doi.org/10.26415/2572-004x-Vol2iss2p199-214(2018)

[5].   Caro-Ordieres, T., Marín-Royo, G., Opazo-Ríos, L., Jiménez-Castilla, L., Moreno, J. A., Gómez-Guerrero, C., & Egido, J., 2020, The Coming Age of Flavonoids in the Treatment of Diabetic Complications. Journal of Clinical Medicine, 9(2): 346. https://doi.org/10.3390/Jcm9020346(2020)

[6].   AL-Ishaq, Abotaleb, Kubatka, Kajo, & Büsselberg, 2019, Flavonoids and their Anti-Diabetic Effects: Cellular Mechanisms and Effects to Improve Blood Sugar Levels. Biomolecules, 9(9): 430. https://doi.org/10.3390/Biom9090430(2019)

[7].   Venugopala, K. N., Chaudhary, J., Sharma, V., Jain, A., Kumar, M., Sharma, D., & Et Al., 2022, Exploring the Potential of Flavonoids as Efflorescing Antidiabetic: An Updated SAR and Mechanistic Based Approach. Pharmacognosy Magazine, 18(80): 791-807. https://www.Phcog.Com/Text.Asp?2022/18/80/791/357236(2022)

[8].   Bai, L., Li, X., He, L., Zheng, Y., Lu, H., Li, J., Zhong, L., Tong, R., Jiang, Z., Shi, J., & Li, J., 2019, Antidiabetic Potential of Flavonoids from Traditional Chinese Medicine: A Review. The American Journal of Chinese Medicine, 47(05): 933–957. https://doi.org/10.1142/S0192415x19500496(2019)

[9].   Sohretoglu, D., & Sari, S., 2019, Flavonoids as Alpha-Glucosidase Inhibitors: Mechanistic Approaches Merged with Enzyme Kinetics and Molecular Modelling. Phytochemistry Reviews, 19(5): 1081–1092. https://doi.org/10.1007/S11101-019-09610-6(2019)

[10].  Ke, R. Q., Wang, Y., Hong, S. H., & Xiao, L. X., 2023, Anti-Diabetic Effect of Quercetin in Type 2 Diabetes Mellitus by Regulating the MicroRNA-92b-3p/EGR1 Axis. Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society, 74(2). https://doi.org/10.26402/Jpp.2023.2.03(2023)

[11].  Ansari, P., Choudhury, S. T., Seidel, V., Rahman, A. B., Aziz, Md. A., Richi, A. E., Rahman, A., Jafrin, U. H., Hannan, J. M. A., & Abdel-Wahab, Y. H. A., 2022, Therapeutic Potential of Quercetin in the Management of Type-2 Diabetes Mellitus. Life, 12(8): 1146. https://doi.org/10.3390/Life12081146(2022)

[12].  Yang, Y., Chen, Z., Zhao, X., Xie, H., Du, L., Gao, H., & Xie, C., 2022, Mechanisms of Kaempferol in the Treatment of Diabetes: A Comprehensive and Latest Review. Frontiers in Endocrinology, 13. https://doi.org/10.3389/Fendo.2022.990299(2022)

[13].  Kumari, G., Nigam, V. K., & Pandey, D. M., 2022, The Molecular Docking and Molecular Dynamics Study of Flavonol Synthase and Flavonoid 3’-Monooxygenase Enzymes Involved for the Enrichment of Kaempferol. Journal of Biomolecular Structure and Dynamics, 41(6): 2478–2491. https://doi.org/10.1080/07391102.2022.2033324(2022)

[14].  Qaddoori, M. H., & Al-Shmgani, H. S., 2023, Galangin-Loaded Gold Nanoparticles: Molecular Mechanisms of Antiangiogenesis Properties in Breast Cancer. International Journal of Breast Cancer, 2023: 1–14. https://doi.org/10.1155/2023/3251211(2023)

[15].  Al Duhaidahawi, D., Hasan, S. A., & Al Zubaidy, H. F. S., 2021, Flavonoids in the Treatment of Diabetes: Clinical Outcomes and Mechanism to Ameliorate Blood Glucose Levels. Current Diabetes Reviews, 17(6). https://doi.org/10.2174/1573399817666201207200346(2021)

[16].  Mathew, M. G., Jeevanandan, G., Vishwanathaiah, S., Hamzi, K. A., Depsh, M. A. N., & Maganur, P. C., 2022, Parental and Child Outlook on the Impact of ECC on Oral Health-Related Quality of Life: A Prospective Interventional Study. The Journal of Contemporary Dental Practice, 23(9), 877–882. https://doi.org/10.5005/Jp-Journals-10024-3397(2022)

[17].  Jayaraman, S., Natarajan, S. R., Veeraraghavan, V. P., 2023, Unveiling the Anti-Cancer Mechanisms of Calotropin: Insights into Cell Growth Inhibition, Cell Cycle Arrest, and Metabolic Regulation in Human Oral Squamous Carcinoma Cells (HSC-3). Journal of Oral Biology and Craniofacial Research, 13(6), 704–713. https://doi.org/10.1016/J.Jobcr.2023.09.002(2023)

[18].  Liao, Y., Hu, X., Pan, J., & Zhang, G., 2022, Inhibitory Mechanism of Baicalein on Acetylcholinesterase: Inhibitory Interaction, Conformational Change, and Computational Simulation. Foods, 11(2): 168. https://doi.org/10.3390/Foods11020168(2022)

[19].  Chan, S.-H., Hung, C.-H., Shih, J.-Y., Chu, P.-M., Cheng, Y.-H., Tsai, Y.-J., Lin, H.-C., & Tsai, K.-L., 2016, Baicalein is an Available Anti-Atherosclerotic Compound Through Modulation of Nitric Oxide-Related Mechanism Under OxLDL Exposure. Oncotarget, 7(28): 42881–42891. https://doi.org/10.18632/Oncotarget.10263 (2016)

[20]. Cazarolli, L., Zanatta, L., Alberton, E., Bonorino Figueiredo, M. S., Folador, P., Damazio, R., Pizzolatti, M., & Barreto Silva, F. R., 2008, Flavonoids: Prospective Drug Candidates. Mini-Reviews in Medicinal Chemistry, 8(13): 1429–1440. https://doi.org/10.2174/138955708786369564(2008)

Abstract:

This study aimed to evaluate the level of awareness and practice of biannual recommended dental clinic visits among the Gweta village population in Botswana.  This was a cross-sectional study. A multivariate analysis was performed utilising binary logistic regression to assess the association between the independent variables under consideration and both awareness and practice of the recommended biannual dental clinic visits (BDCV). Of the 386 participants, one-third (n = 66, 29.7) were aware of the recommended BDCV, and approximately two-thirds (n = 131, 59.0%) reported attending a dental clinic twice a year. Among those who reported being aware of the recommended BDCV, only 25 (11.3%) implemented the recommendation. More than half (n = 227, 58.8%) of individuals reported good toothbrushing oral hygiene practice, and 288 (74.6%) indicated appropriate toothbrushing oral hygiene frequency (two times per day). One hundred and seventy-seven participants (45.9%) reported good dental hygiene practices. Approximately three in ten (n = 137, 35.5%) individuals reported smoking, whereas six in ten (n = 241, 62.4%) reported using alcohol. There was a significant difference in the percentage of people who identified the importance of oral health for quality of life (94.1% vs. 5.1%, p = 0.009). Individuals who recognised the impact of oral health on quality of life were found to have a threefold higher likelihood of being aware of and adhering to the recommended biannual dental clinic visits (OR = 3.086, 95% CI 1.179–8.077). We propose a health system research project to implement evidence-based health promotion and education programs to improve oral health services and lifestyles.

References:

[1].   World Health Organisation, 2022, Global Oral Health Status Report: Towards Universal Health Coverage for Oral Health by 2030.

[2].   Dosumu, T. O., Betiku, A., Ademuyiwa, G., Olabisi, O., Adebisi, T., & Oyekale, R., 2022, Oral Hygiene Practices and Factors Influencing the Choice of Oral Hygiene Materials Among Undergraduates in Selected Universities in Osun State, Nigeria, Journal of Pre-Clinical and Clinical Research, 16(4), 137–142.

[3].   Umanah, A., Braimoh, O., 2017, Oral Hygiene Practices and Factors Influencing the Choice of Oral Hygiene Materials Among Undergraduate Students at the University of Port Harcourt, Rivers State, Nigeria, Journal of Dental and Allied Sciences, 6, 3.

[4].   Anthonappa, R., King, N., 2008, Six-Month Recall Dental Appointments, for all Children, are (un)Justifiable, Journal of Clinical Pediatric Dentistry, 33(1), 1–8.

[5].   Amarasena, N., Luzzi, L., Brennan, D., 2023, Effect of Different Frequencies of Dental Visits on Dental Caries and Periodontal Disease: A Scoping Review, International Journal of Environmental Research and Public Health, 20(19), 6858.

[6].   Fee, P. A., Riley, P., Worthington, H. V., Clarkson, J. E., Boyers, D., & Beirne, P. V., 2020, Recall Intervals for Oral Health in Primary Care Patients, Cochrane Database of Systematic Reviews, 2020 (10).

[7].   Glick, M., da Silva, O. M., Seeberger, G. K., Xu, T., Pucca, G., Williams, D. M., Séverin, T., 2012, FDI Vision 2020: Shaping the Future of Oral Health, International dental journal, 62(6), 278.

[8].   Woolfolk, M. W., Lang, W. P., Borgnakke, W. S., Taylor, G. W., Ronis, D. L., Nyquist, L. V., 1999, Determining Dental Checkup Frequency, The Journal of the American Dental Association, 130(5), 715–723.

[9].   Schneider, C., Zemp, E., & Zitzmann, N. U., 2019, Dental Care Behaviour in Switzerland. Swiss Dent J, 129(6), 466–478.

[10].  Kuthy, R. A., Kavand, G., Momany, E. T., Jones, M. P., Askelson, N. M., Chi, D. L., Damiano, P. C., 2013, Periodicity of Dental Recall Visits for Young Children First Seen in Community Health Centers. Journal of Public Health Dentistry, 73(4), 271–279.

[11].  Jessani, A., Quadri, M. F. A., Lefoka, P., El-Rabbany, A., Hooper, K., Lim, H. J., Laronde, D. M., 2021, Oral Health Status and Patterns of Dental Service Utilisation of Adolescents in Lesotho, Southern Africa, Children, 8(2), 120.

[12].  Sohn, W., Ismail, A., Amaya, A., & Lepkowski, J., 2007, Determinants of Dental Care Visits Among Low-Income African-American Children. The Journal of the American Dental Association, 138(3), 309–318.

[13].  Varenne, B., Msellati, P., Zoungrana, C., Fournet, F., & Salem, G., 2005, Reasons for Attending Dental-Care Services in Ouagadougou, Burkina Faso, Bulletin of the World Health Organisation, 83(9), 650–655.

[14].  Yumpu.com. (n.d.). 2011, Population and Housing Census. Yumpu.Com. Retrieved, 2024, from https://www.yumpu.com/en/document/view/63039123/2011-population-and-housing-census

[15].  Bartlett, J. E., Kotrlik, J. W., & Higgins, C. C., (n.d.). Organisational Research: Determining Appropriate Sample Size in Survey Research.

[16].  Botswana Ministry of Health, 2010, Botswana Essential Health Services Packages.

[17].  MacKian, S., n. d. A review of Health Seeking Behaviour: Problems and Prospects. University of Manchester, Health Systems Development Programme.

[18].  Hanemann, U., 2006, Literacy in Botswana. Paper Commissioned for the EFA Global Monitoring Report.

[19].  FDI World Dental Federation, 2024, Alcohol as a Risk for Oral Health, International Dental Journal, 74(1), 165–166.

[20].  Baiju, R., Peter, E., Varghese, N., & Sivaram, R., 2017, Oral Health and Quality of Life: Current Concepts, Journal of Clinical and Diagnostic Research : JCDR, 11(6), ZE21–ZE26.

[21].  Yap, A., 2017, Oral Health Equals Total Health: A Brief Review, Journal of Dentistry Indonesia, 24(2), 59–62.

Abstract:

One of the most prevalent burn sequelae is postburn contracture (PBC). Contractures involving the neck lead to severe deformities as well as functional debilitation like loss of neck movements and lip seals. Moreover, cosmetic disfigurement has social implications which affect the daily life of the patient. It is a surgical challenge to release the contracture and reconstruct the area by giving a good cosmetic outcome as well as restoring the functions.

References:

[1].   Burd, A., 2010, Burns: Treatment and Outcomes, Semin Plast Surg, Aug 24(3), 262-80.

[2].   Goel, A., 2010, Post-Burn Scars and Scar Contractures, Indian J Plast Surg, Sep 43(Suppl), S63-71.

[3].   Bordoni, B., 2013, Skin, Fascias, and Scars: Symptoms and Systemic Connections, J Multidiscip Healthc, Dec 28, 7, 11-24.

[4].   Valente, S. M., 2004, Visual Disfigurement and Depression, Plast Surg Nurs, Oct-Dec 24(4), 140-6, Quiz 147-8.

[5].   Al-Himdani, S., 2017, Tissue-Engineered Solutions in Plastic and Reconstructive Surgery: Principles and Practice, Front Surg, Feb 23, 4:4.

[6].   Otene, C. I., 2011, Donor Site Morbidity Following Harvest of Split-Thickness Skin Grafts in South Eastern Nigeria, J West Afr Coll Surg, Apr-Jun, 1(2), 86-96.

[7].   Prohaska, J., 2024, Skin Grafting, Stat Pearls [Internet], Treasure Island [FL], Stat Pearls Publishing.

[8].   Ali, H., 2019, Post-Burn Neck Contracture: Effectively Managed with Supraclavicular Artery Flap, Ann Burns Fire Disasters. Dec 31;32(4), 301-307.

[9].   Fanstone, R., 2024, Risk Factors for Burn Contractures in a Lower Income Country: Four Illustrative Cases, Scars Burn Heal, Mar 12, 10:20595131241236190.

[10].  Finnerty, C. C., 2016, Hypertrophic Scarring: The Greatest Unmet Challenge After Burn Injury, Lancet, Oct 1, 388(10052), 1427-1436.

[11].  Hayashida, K., 2017, Surgical Treatment Algorithms for Post-Burn Contractures, Burns Trauma, Mar 14, 5:9.

[12].  Ogawa, R., 2019, Diagnosis and Treatment of Keloids and Hypertrophic Scars-Japan Scar Workshop Consensus Document 2018, Burns Trauma, Dec 27, 7:39.

[13].  Abrams, R., 2024, Shoulder Dislocations Overview, Stat Pearls [Internet], Treasure Island (FL), StatPearls Publishing.

[14].  Kazemian, G. H., 2016, External Fixation vs. Skeletal Traction for Treatment of Intertrochanteric Fractures in the Elderly, Trauma Mon, Feb 6, 21(1), e15477.

[15].  Akhtar, M. S., 2014, Versatility of Pedicled Tensor Fascia Lata Flap: A Useful and Reliable Technique for Reconstruction of Different Anatomical Districts, Plast Surg Int, 2014, 846082.

[16].  Wagh, M. S., 2013, Tissue Expansion: Concepts, Techniques and Unfavourable Results, Indian J Plast Surg, May 46(2), 333-48.

[17].  Alghamdi, H., 2009, Surgical Management of Gingival Recession: A Clinical Update, J Saudi Dent, Jul, 21(2), 83-94.

[18].  Veltman, E. S., 2015, Static Progressive Versus Dynamic Splinting for Posttraumatic Elbow Stiffness: A Systematic Review of 232 Patients, Arch Orthop Trauma Surg, May, 135(5), 613-7.

Abstract:

Lack of adequate sleep and irregular sleep patterns stand as independent factors contributing to chronic inflammation. Sleep deprivation also correlates with the onset of various neurodegenerative diseases, marked by brain protein accumulation leading to memory loss and cognitive decline. This study aims to investigate the connection between sleep deprivation and the pro-inflammatory markers aiming to enhance our comprehension of this relationship and potentially identify future intervention possibilities. The study was conducted with 50 individuals as participants of which 25 were sleep-deprived and 25 had adequate amounts of sleep. The sleep duration details of the individuals were obtained by questionnaire. Blood was withdrawn from all the subjects after due consent from them. Plasma levels of S-100, acetylcholinesterase (AchE), C-reactive protein (CRP) and Complement 3 (C3) were assessed using the ELISA method in the fasted state. The results demonstrated a significant positive correlation between acute sleep deprivation and increased levels of S-100, AchE, CRP and C3 levels in the sleep-deprived individuals when compared to individuals who had adequate sleep. Our exploratory study results suggest that sleep deprivation is associated with increased levels of pro-inflammatory markers in the body. These changes reinforce the notion that sleep deprivation may have detrimental effects on brain health, even in younger individuals. It's essential to study larger groups to distinguish between the impacts of sleep loss and circadian rhythms, understand implications for persistent conditions like those in shift workers, and explore how these effects might interact with other lifestyle choices and genetic factors.

References:

[1]. Bentivoglio, M., and Grassi-Zucconi, G., 1997, The Pioneering Experimental Studies on Sleep Deprivation. Sleep, 20(7), pp.570-576.

[2]. Jenkins, J. G., and Dallenbach, K. M., 1924, Obliviscence During Sleep and Waking. The American Journal of Psychology, 35(4), pp.605-612.

[3]. Cedernaes, J., Osorio, R. S., Varga, A. W., Kam, K., Schiöth, H. B., and Benedict, C., 2017, Candidate Mechanisms Underlying the Association Between Sleep-Wake Disruptions and Alzheimer's Disease. Sleep Medicine Reviews, 31, pp.102-111.

[4]. Becker, S. P., Sidol, C. A., Van Dyk, T. R., Epstein, J. N., and Beebe, D. W., 2017, Intraindividual Variability of Sleep/Wake Patterns about Child and Adolescent Functioning: A Systematic Review. Sleep Medicine Reviews,34, pp.94-121.

[5]. Burman, D., Ramanujam, K., Manzar, D., Chattu, V.K., Spence, D.W., Zaki, N.F., Jahrami, H. and Pandi-Perumal, S.R., 2023. Sleep and autism spectrum disorder: A comprehensive review of diagnosis, markers, interventions, and treatments. Sleep and Vigilance, 7(1), pp.9-22.

[6]. Dzierzewski, J. M., Donovan, E. K., Kay, D. B., Sannes, T. S., and Bradbrook, K. E., 2020, Sleep Inconsistency and Markers of Inflammation. Frontiers in Neurology, 11, p.1042.

[7]. Frey, D. J., Fleshner, M., and Wright Jr, K. P., 2007, The Effects of 40 Hours of Total Sleep Deprivation on Inflammatory Markers in Healthy Young Adults. Brain, Behavior, and Immunity, 21(8), pp.1050-1057.

[8]. Wright Jr, K. P., Drake, A. L., Frey, D. J., Fleshner, M., Desouza, C. A., Gronfier, C., and Czeisler, C. A., 2015, Influence of Sleep Deprivation and Circadian Misalignment on Cortisol, Inflammatory Markers, and Cytokine Balance. Brain, Behavior, and Immunity, 47, pp.24-34.

[9]. Mander, B. A., Winer, J. R., Jagust, W. J., and Walker, M. P., 2016, Sleep: A Novel Mechanistic Pathway, Biomarker, and Treatment Target in the Pathology of Alzheimer's Disease?. Trends in Neurosciences, 39(8), pp.552-566.

[10]. Bubu, O. M., Brannick, M., Mortimer, J., Umasabor-Bubu, O., Sebastião, Y. V., Wen, Y., Schwartz, S., Borenstein, A. R., Wu, Y., Morgan, D., and Anderson, W. M., 2017, Sleep, Cognitive Impairment, and Alzheimer’s Disease: A Systematic Review and Meta-Analysis. Sleep, 40(1), p. zsw032.

[11]. Pandi-Perumal, S.R., Cardinali, D.P., Zaki, N.F., Karthikeyan, R., Spence, D.W., Reiter, R.J. and Brown, G.M., 2022. Timing is everything: Circadian rhythms and their role in the control of sleep. Frontiers in neuroendocrinology, 66, p.100978.

[12]. Irwin, M. R. and Vitiello, M. V., 2019, Implications of Sleep Disturbance and Inflammation for Alzheimer's Disease Dementia. The Lancet Neurology, 18(3), pp.296-306.

[13]. Patel, S. R., Zhu, X., Storfer-Isser, A., Mehra, R., Jenny, N. S., Tracy, R., and Redline, S., 2009, Sleep Duration and Biomarkers of Inflammation. Sleep, 32(2), pp.200-204.

[14]. Mullington, J. M., Simpson, N. S., Meier-Ewert, H. K., and Haack, M., 2010, Sleep Loss and Inflammation. Best Practice & Research Clinical Endocrinology & Metabolism, 24(5), pp.775-784.

[15]. Haack, M., Sanchez, E., and Mullington, J. M., 2007, Elevated Inflammatory Markers in Response to Prolonged Sleep Restriction are Associated with Increased Pain Experience in Healthy Volunteers. Sleep, 30(9), pp.1145-1152.

[16]. Meier-Ewert, H. K., Ridker, P. M., Rifai, N., Regan, M. M., Price, N. J., Dinges, D. F., and Mullington, J. M., 2004, Effect of Sleep Loss on C-Reactive Protein, an Inflammatory Marker of Cardiovascular Risk. Journal of the American College of Cardiology, 43(4), pp.678-683.

[17]. Aldabbas, M.M., Tanwar, T., Ghrouz, A., Iram, I., Warren Spence, D., Pandi‐Perumal, S.R. and Veqar, Z., 2022. A polysomnographic study of sleep disruptions in individuals with chronic neck pain. Journal of Sleep Research, 31(5), p.e13549.

[18]. Goswami, D., Anuradha, U., Angati, A., Kumari, N., and Singh, R. K., 2024, Pharmacological And Pathological Relevance of S100 Proteins in Neurological Disorders. CNS & Neurological Disorders-Drug Targets (Formerly Current Drug Targets-CNS & Neurological Disorders).

[19]. Juanjuan, L., 2019, Clinical Study of Plasma GABA, Glu and Ach Levels in Patients with Insomnia Combined with Cerebral Infarction. Med. Innov. China, 16, pp.37-40.

[20]. Kasperska-Zajac, A., Grzanka, A., Machura, E., Misiolek, M., Mazur, B., and Jochem, J., 2013, Increased Serum Complement C3 and C4 Concentrations and their Relation to Severity of Chronic Spontaneous Urticaria and CRP concentration. Journal of Inflammation, 10, pp.1-5.

[21]. Copenhaver, M. M., Yu, C. Y., Zhou, D., and Hoffman, R. P., 2020, Relationships of Complement Components C3 and C4 and their Genetics to Cardiometabolic Risk in Healthy, Non-Hispanic White Adolescents. Pediatric research, 87(1), pp.88-94.

[22]. Liu, Z., Tang, Q., Wen, J., Tang, Y., Huang, D., Huang, Y., Xie, J., Luo, Y., Liang, M., Wu, C., and Lu, Z., 2016, Elevated Serum Complement Factors 3 and 4 are Strong Inflammatory Markers of the Metabolic Syndrome Development: A Longitudinal Cohort Study. Scientific Reports, 6(1), p.18713.

[23]. Howren, M. B., Lamkin, D. M., and Suls, J., 2009, Associations of Depression with C-Reactive Protein, IL-1, and IL-6: A Meta-Analysis. Psychosomatic Medicine, 71(2), pp.171-186.

[24]. Krysta, K., Krzystanek, M., Bratek, A., and Krupka-Matuszczyk, I., 2017, Sleep and Inflammatory Markers in Different Psychiatric Disorders. Journal of Neural Transmission, 124, pp.179-186.

[25]. Goldstein, F. C., Zhao, L., Steenland, K., and Levey, A. I., 2015, Inflammation and Cognitive Functioning in African Americans and Caucasians. International Journal of Geriatric Psychiatry, 30(9), pp.934-941.

[26]. Jefferson, A. L., Massaro, J. M., Beiser, A. S., Seshadri, S., Larson, M. G., Wolf, P. A., Au, R., and Benjamin, E. J., 2011, Inflammatory Markers and Neuropsychological Functioning: the Framingham Heart Study. Neuroepidemiology, 37(1), pp.21-30.

[27]. Pak, V. M., Paul, S., Swieboda, D., Balthazar, M. S., and Wharton, W., 2022, Sleep Duration and Biomarkers of Inflammation in African American and White Participants with a Parental History of Alzheimer's Disease. Alzheimer's & Dementia: Translational Research & Clinical Interventions, 8(1), p.e12332.

[28]. Suarez, E. C., 2008, Self-Reported Symptoms of Sleep Disturbance and Inflammation, Coagulation, Insulin Resistance and Psychosocial Distress: Evidence for Gender Disparity. Brain, Behavior, and Immunity, 22(6), pp.960-968.

[29]. Hale, L., Parente, V., Dowd, J. B., Sands, M., Berger, J. S., Song, Y., Martin, L.W., and Allison, M. A., 2013, Fibrinogen May Mediate the Association Between Long Sleep Duration and Coronary Heart Disease. Journal of Sleep Research, 22(3), pp.305-314.

[30]. Hui, L., Hua, F., Diandong, H., and Hong, Y., 2007, Effects of Sleep and Sleep Deprivation on Immunoglobulins and Complement in Humans. Brain, Behavior, and Immunity, 21(3), pp.308-310.

[31]. Wadhwa, M., Prabhakar, A., Anand, J. P., Ray, K., Prasad, D., Kumar, B., and Panjwani, U., 2019, Complement Activation Sustains Neuroinflammation and Deteriorates Adult Neurogenesis and Spatial Memory Impairment in Rat Hippocampus Following Sleep Deprivation. Brain, Behavior, and Immunity, 82, pp.129-144.

[32]. Reis, E. S., Lange, T., Köhl, G., Herrmann, A., Tschulakow, A. V., Naujoks, J., Born, J., and Köhl, J., 2011. Sleep and Circadian Rhythm Regulate Circulating Complement Factors and Immunoregulatory Properties of C5a. Brain, Behavior, and Immunity, 25(7), pp.1416-1426.

[33]. Zucheng, L., Zechun, K., Shucui, L., and Yuanfang, W., 2016, Effects of Caffeine on Learning Memory and Hippocampal Antioxidant Capacity and Cholinergic System in Sleep Deprived Aged Mice Cholinergic System.Chin. J. Gerontol, 36, pp.2583-2585.

[34]. Tong, L., Shumei, X., 2007, Effect of Sleep Deprivation on Ach Level in Hippocampus and Learning Ability of Rats. J. Clin. Exp. Med, 31, pp.12–13.

[35]. Chen, P., Ban, W., Wang, W., You, Y., and Yang, Z., 2023, The Devastating Effects of Sleep Deprivation on Memory: Lessons from Rodent Models. Clocks & Sleep, 5(2), pp.276-294.

[36]. Sharma, B., Roy, A., Sengupta, T., Vishwakarma, L. C., Singh, A., Netam, R., Nag, T. C., Akhtar, N., and Mallick, H. N., 2023, Acute Sleep Deprivation Induces Synaptic Remodeling at the Soleus Muscle Neuromuscular Junction in Rats. Sleep, 46(8), p. zsac229.

[37]. Benedict, C., Cedernaes, J., Giedraitis, V., Nilsson, E. K., Hogenkamp, P. S., Vågesjö, E., Massena, S., Pettersson, U., Christoffersson, G., Phillipson, M., and Broman, J. E., 2014, Acute Sleep Deprivation Increases Serum Levels of Neuron-Specific Enolase (NSE) and S100 Calcium Binding Protein B (S-100B) in Healthy Young Men. Sleep, 37(1), pp.195-198.

[38]. Mccord, J. M., 1993, Human Disease, Free Radicals, and the Oxidant/Antioxidant Balance. Clinical Biochemistry, 26(5), pp.351-357.

[39]. Fukui, K., Takatsu, H., Koike, T., and Urano, S., 2011, Hydrogen Peroxide Induces Neurite Degeneration: Prevention by Tocotrienols. Free Radical Research, 45(6), pp.681-691.

[40]. Moreira, G. G., Cantrelle, F. X., Quezada, A., Carvalho, F. S., Cristóvão, J. S., Sengupta, U., Puangmalai, N., Carapeto, A. P., Rodrigues, M. S., Cardoso, I., and Fritz, G., 2021, Dynamic Interactions and Ca2+-Binding Modulate the Holdase-Type Chaperone Activity of S100B Preventing Tau Aggregation and Seeding. Nature communications, 12(1), p.6292.

[41]. DeVos, S. L., Corjuc, B. T., Oakley, D. H., Nobuhara, C. K., Bannon, R. N., Chase, A., Commins, C., Gonzalez, J. A., Dooley, P. M., Frosch, M. P., and Hyman, B. T., 2018, Synaptic Tau Seeding Precedes Tau Pathology in Human Alzheimer's Disease Brain. Frontiers in Neuroscience, 12, p.267.

[42]. Donato, R., Cannon, B., Sorci, G., Riuzzi, F., Hsu, K. J., Weber, D., et al. 2013, Functions of S100 Proteins. Curr Mol Med, 13(1), pp.24-57.

Abstract:

Human saliva is a complex mixture of various organic and inorganic compounds and host-derived molecules. Performs numerous functions. This comprehensive review will discuss the roles played by saliva in defence against the SARS-CoV-2 virus and the use of saliva as a diagnostic fluid in COVID-19 screening will be discussed along with a brief note on SARS-CoV-2 transmission through saliva. Saliva and SARS Cov 2: The antimicrobial and antiviral properties of saliva are conferred by the salivary peptides such as defensins, cathelicidins, and LL 37. Antiviral activity against the herpes virus, hepatitis C virus, ebola virus and to an extent HIV has been documented. Since the COVID-19 pandemic has now occurred as a new global threat, it is being investigated if saliva has certain properties that could defend against this infection. Studies have found the regular presence of the SARS-CoV-2 virus, the aetiological agent of the COVID-19 disease in saliva, hence saliva could be used as a diagnostic tool. Some interesting findings have highlighted the presence of the virus in salivary samples but documented its absence in throat swabs which is intriguing. Despite having multifaceted roles, the drawback of saliva also lies in its contribution to the transmission of the SARS-CoV-2 virus. Studies have shown that viable viruses can be transmitted through saliva from person to person through coughing and sneezing. Hence saliva could be regarded as a double-edged sword in the COVID-19 pandemic.

References:

[1].   Anderson, P., Hector, M. P., & Rampersad, M. A., 2001, Critical pH in Resting and Stimulated Whole Saliva in Groups of Children and Adults. International Journal of Paediatric Dentistry, 11(4), 266–273, https://doi.org/10.1046/j.1365-263x.2001.00293.x

[2].   Alamoudi, N., Farsi, N., Faris, J., Masoud, I., Merdad, K., and Meisha, D., 2004, Salivary Characteristics of Children and its Relation to Oral Microorganism and Lip Mucosa Dryness. The Journal of Clinical Paediatric Dentistry, 28(3), 239–248. https://doi.org/10.17796/jcpd.28.3.h24774507006l550

[3].   Baskar, S. N., 1997, Orban’s Oral Histology and Embryology. (11th ed. St. Louis: Harcourt Asia PIE Ltd., Mosby).

[4].   Edgar, W. M.,1992, Saliva: Its Secretion, Composition and Functions. British Dental Journal, 172(8), 305–312, https://doi.org/10.1038/sj.bdj.4807861

[5].   Roth, G., Calmes, R., 1981, Salivary Glands and Saliva. Oral Biology, (CV Mosby, St Louis).

[6].   Edgar, W. M., 1990, Saliva and Dental Health. Clinical Implications of Saliva: Report of a Consensus Meeting. British Dental Journal, 169(3-4), 96–98, https://doi.org/10.1038/sj.bdj.4807284

[7].   K, H. S., R, G., Ramani, P., & Veeraraghavan, V. P., 2024, Longitudinal Study on Salivary IL-6 Trajectories in Postoperative OSCC Patients After Chemotherapy and Radiotherapy. Journal of Stomatology, Oral and Maxillofacial Surgery, 101909. Advance Online Publication. https://doi.org/10.1016/j.jormas.2024.101909

[8].   Alam, M. K., Zaman, M. U., Alqhtani, N. R., Alqahtani, A. S., Alqahtani, F., Cicciù, M., & Minervini, G., 2024, Salivary Biomarkers and Temporomandibular Disorders: A Systematic Review Conducted According to PRISMA Guidelines and the Cochrane Handbook for Systematic Reviews of Interventions. Journal of Oral Rehabilitation, 51(2), 416–426. https://doi.org/10.1111/joor.13589

[9].   Thomas, J. T., Joseph, B., Varghese, S., Thomas, N. G., Kamalasanan Vijayakumary, B., Sorsa, T., Anil, S., & Waltimo, T., 2024, Association Between Metabolic Syndrome and Salivary MMP-8, Myeloperoxidase in Periodontitis. Oral Diseases, Advance Online Publication. https://doi.org/10.1111/odi.15014

[10].  Fathima, R., Ramamoorthi, R., Gopalakrishnan, S., Jayaseelan, V. P., & Muniapillai, S., 2024, Expression of Salivary Levels of S100A7 in Oral Submucous Fibrosis and Oral Leukoplakia. Journal of Oral and Maxillofacial Pathology: JOMFP, 28(1), 84–89. https://doi.org/10.4103/jomfp.jomfp_113_23

[11].  Dawes, C., 1998, Recent Research on Calculus. The New Zealand Dental Journal, 94(416), 60–62.

[12].  Malamud, D., Abrams, W. R., Barber, C. A., Weissman, D., Rehtanz, M., & Golub, E., 2011, Antiviral Activities in Human Saliva. Advances in Dental Research, 23(1), 34–37. https://doi.org/10.1177/0022034511399282

[13].  Wu, Z., Van, Ryk, D., Davis, C., Abrams, W. R., Chaiken, I., Magnani, J., & Malamud, D., 2003, Salivary Agglutinin Inhibits HIV Type 1 Infectivity Through Interaction with Viral Glycoprotein 120. AIDS Research and Human Retroviruses, 19(3), 201–209, https://doi.org/10.1089/088922203763315704

[14].  White, M. R., Crouch, E., Vesona, J., Tacken, P. J., Batenburg, J. J., Leth-Larsen, R., Holmskov, U., and Hartshorn, K. L., 2005, Respiratory Innate Immune Proteins Differentially Modulate the Neutrophil Respiratory Burst Response to Influenza a Virus. Lung Cellular and Molecular Physiology. American Journal of Physiology, 289(4), L606–L616, https://doi.org/10.1152/ajplung.00130.2005

[15].  Nagashunmugam, T., Malamud, D., Davis, C., Abrams, W. R., & Friedman, H. M.,1998, Human Submandibular Saliva Inhibits Human Immunodeficiency Virus Type 1 Infection by Displacing Envelope Glycoprotein gp120 from the Virus. The Journal of Infectious Diseases, 178(6), 1635–1641, https://doi.org/10.1086/314511

[16].  Banerjee, A., Kulcsar, K., Misra, V., Frieman, M., & Mossman, K., 2019, Bats and Coronaviruses. Viruses, 11(1), 41, https://doi.org/10.3390/v11010041

[17].  Yang, D., & Leibowitz, J. L., 2015, The Structure and Functions of Coronavirus Genomic 3' and 5' Ends. Virus Research, 206, 120–133, https://doi.org/10.1016/j.virusres.2015.02.025

[18].  Ramaiah. A., Arumugaswami, V., 2020, Insights into Cross-Species Evolution of Novel Human Coronavirus 2019-nCoV and Defining Immune Determinants for Vaccine Development. bioRxiv https://doi:10.1101/2020.01.29.925867

[19].  Chan, J. F., Kok, K. H., Zhu, Z., Chu, H., To, K. K., Yuan, S., and Yuen, K. Y., 2020, Genomic Characterization of the 2019 Novel Human-Pathogenic Coronavirus Isolated from a Patient with Atypical Pneumonia After Visiting Wuhan. Emerging Microbes & Infections, 9(1), 221–236, https://doi.org/10.1080/22221751.2020.1719902

[20].  Wu, A., Peng, Y., Huang, B., Ding, X., Wang, X., Niu, P., Meng, J., Zhu, Z., Zhang, Z., Wang, J., Sheng, J., Quan, L., Xia, Z., Tan, W., Cheng, G., & Jiang, T., 2020, Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China. Cell Host & Microbe, 27(3), 325–328, https://doi.org/10.1016/j.chom.2020.02.001

[21].  Yuan, Y., Cao, D., Zhang, Y., Ma, J., Qi, J., Wang. Q., et al., 2017, Cryo-EM Structures of MERS-CoV and SARS-CoV Spike Glycoproteins Reveal the Dynamic Receptor Binding Domains. Nat Commun, 8:15092, https://doi:10.1038/ncomms15092

[22].  Walls, A. C., Xiong, X., Park, Y. J., Tortorici, M. A., Snijder, J., Quispe, J., Cameroni, E., Gopal, R., Dai, M., Lanzavecchia, A., Zambon, M., Rey, F. A., Corti, D., & Veesler, D., 2019, Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion. Cell, 176(5), 1026–1039.e15. https://doi.org/10.1016/j.cell.2018.12.028

[23].  Paules, C. I., Marston, H, D., & Fauci, A. S., 2020, Coronavirus Infections-More than Just the Common Cold. JAMA, 323(8), 707–708, https://doi.org/10.1001/jama.2020.0757

[24].  Xu, H., Zhong, L., Deng, J., Peng, J., Dan, H., Zeng, X., Li, T., & Chen, Q., 2020, High Expression of ACE2 Receptor of 2019-nCoV on the Epithelial Cells of Oral Mucosa. International Journal of Oral Science, 12(1), 8, https://doi.org/10.1038/s41368-020-0074-x

[25].  Iwabuchi, H., Fujibayashi, T., Yamane, G. Y., Imai, H., & Nakao, H., 2012, Relationship Between Hyposalivation and Acute Respiratory Infection in Dental Outpatients. Gerontology, 58(3), 205–211. https://doi.org/10.1159/000333147

[26].  Magister, S., & Kos, J., 2013, Cystatins in Immune System. Journal of Cancer, 4(1), 45–56. https://doi.org/10.7150/jca.5044

[27].  Collins, A. R., & Grubb, A., Cystatin, D., 1998, A Natural Salivary Cysteine Protease Inhibitor, Inhibits Coronavirus Replication at its Physiologic Concentration. Oral Microbiology and Immunology, 13(1), 59–61, https://doi.org/10.1111/j.1399-302x.1998.tb00753.x

[28].  Dawes, C., Pedersen, A. M., Villa, A., Ekström, J., Proctor, G. B., Vissink, A., Aframian, D., McGowan, R., Aliko, A., Narayana, N., Sia, Y. W., Joshi, R. K., Jensen, S. B., Kerr, A. R., & Wolff, A., 2015, The Functions of Human Saliva: A Review Sponsored by the World Workshop on Oral Medicine VI. Archives of Oral Biology, 60(6), 863–874, https://doi.org/10.1016/j.archoralbio.2015.03.004

[29].  Irmak, M. K., Erdem, U., & Kubar, A., 2012, Antiviral Activity of Salivary microRNAs for Ophthalmic Herpes Zoster. Theoretical Biology and Medical Modelling, 9(1), 21, https://doi.org/10.1186/1742‐4682‐9‐21

[30].  Baghizadeh Fini, M., 2020, Oral Saliva and COVID-19. Oral Oncology, 108, 104821. https://doi.org/10.1016/j.oraloncology.2020.104821

[31].  Centers for Disease Control and Prevention Transmission of Coronavirus Disease 2019 (COVID-19). Accessed 18th Mar 2020, Available at: https://www.cdc.gov/coronavirus/2019-ncov/about/transmission.html

[32].  To, K. K., Tsang, O. T., Yip, C. C., Chan, K. H., Wu, T. C., Chan, J. M., Leung, W. S., Chik, T. S., Choi, C. Y., Kandamby, D. H., Lung, D. C., Tam, A. R., Poon, R. W., Fung, A. Y., Hung, I. F., Cheng, V. C., Chan, J. F., & Yuen, K. Y., 2020, Consistent Detection of 2019 Novel Coronavirus in Saliva. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 71(15), 841–843. https://doi.org/10.1093/cid/ciaa149

[33].  Hong, K. H., Lee, S. W., Kim, T. S., Huh, H. J., Lee, J., Kim, S. Y., Park, J. S., Kim, G. J., Sung, H., Roh, K. H., Kim, J. S., Kim, H. S., Lee, S. T., Seong, M. W., Ryoo, N., Lee, H., Kwon, K. C., & Yoo, C. K., 2020, Guidelines for Laboratory Diagnosis of Coronavirus Disease 2019 (COVID-19) in Korea. Annals of Laboratory Medicine, 40(5), 351–360. https://doi.org/10.3343/alm.2020.40.5.351

[34].  Ng, K., Poon, B. H., Kiat Puar, T. H., Shan Quah, J. L., Loh, W. J., Wong, Y. J., Tan, T. Y., & Raghuram, J., 2020, COVID-19 and the Risk to Health Care Workers: A Case Report. Annals of Internal Medicine, 172(11), 766–767. https://doi.org/10.7326/L20-0175

[35].  Chojnowska, S., Baran, T., Wilińska, I., Sienicka, P., Cabaj-Wiater, I., & Knaś, M., 2018, Human Saliva as a Diagnostic Material. Advances in Medical Sciences, 63(1), 185–191, https://doi.org/10.1016/j.advms.2017.11.002

[36].  To, K. K., Tsang, O. T., Yip, C. C., Chan, K. H., Wu, T. C., Chan, J. M., Leung, W. S., Chik, T. S., Choi, C. Y., Kandamby, D. H., Lung, D. C., Tam, A. R., Poon, R. W., Fung, A. Y., Hung, I. F., Cheng, V. C., Chan, J. F., & Yuen, K. Y., 2020, Consistent Detection of 2019 Novel Coronavirus in Saliva. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 71(15), 841–843. https://doi.org/10.1093/cid/ciaa149

[37].  To, K. K., Tsang, O. T., Leung, W. S., Tam, A. R., Wu, T. C., Lung, D. C., Yip, C. C., Cai, J. P., Chan, J. M., Chik, T. S., Lau, D. P., Choi, C. Y., Chen, L. L., Chan, W. M., Chan, K. H., Ip, J. D., Ng, A. C., Poon, R. W., Luo, C. T., Cheng, V. C., Yuen, K. Y., 2020, Temporal Profiles of Viral Load in Posterior Oropharyngeal Saliva Samples and Serum Antibody Responses During Infection by SARS-CoV-2: An Observational Cohort Study. The Lancet. Infectious diseases, 20(5), 565–574, https://doi.org/10.1016/S1473-3099(20)30196-1

[38].  Azzi, L., Carcano, G., Gianfagna, F., Grossi, P., Gasperina, D. D., Genoni, A., Fasano, M., Sessa, F., Tettamanti, L., Carinci, F., Maurino, V., Rossi, A., Tagliabue, A., & Baj, A., 2020, Saliva is a Reliable Tool to Detect SARS-CoV-2. The Journal of Infection, 81(1), e45–e50, https://doi.org/10.1016/j.jinf.2020.04.005

[39].  Han, M. S., Seong, M. W., Heo, E. Y., Park, J. H., Kim, N., Shin, S., Cho, S. I., Park, S. S., & Choi, E. H., 2020, Sequential Analysis of Viral Load in a Neonate and Her Mother Infected With Severe Acute Respiratory Syndrome Coronavirus 2. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 71(16), 2236–2239, https://doi.org/10.1093/cid/ciaa447

[40].  Wan, S., Xiang, Y., Fang, W., Zheng, Y., Li, B., Hu, Y., Lang, C., Huang, D., Sun, Q., Xiong, Y., Huang, X., Lv, J., Luo, Y., Shen, L., Yang, H., Huang, G., & Yang, R., 2020, Clinical Features and Treatment of COVID-19 Patients in Northeast Chongqing. Journal of Medical Virology, 92(7), 797–806, https://doi.org/10.1002/jmv.25783

[41].  Peng, Y. D., Meng, K., Guan, H. Q., Leng, L., Zhu, R. R., Wang, B. Y., He, M. A., Cheng, L. X., Huang, K., & Zeng, Q. T., 2020, Zhonghua Xin Xue Guan Bing Za Zhi, 48(6), 450–455, https://doi.org/10.3760/cma.j.cn112148-20200220-00105

[42].  Cerón, J. J., Martinez-Subiela, S., Ohno, K., & Caldin, M., 2008, A Seven-Point Plan for Acute Phase Protein Interpretation in Companion Animals. Veterinary Journal (London, England: 1997), 177(1), 6–7, https://doi.org/10.1016/j.tvjl.2007.12.001

[43].  Wan, S., Yi, Q., Fan, S., Lv, J., Zhang, X., Guo, L., Lang, C., Xiao, Q., Xiao, K., Yi, Z., Qiang, M., Xiang, J., Zhang, B., Chen, Y., & Gao, C., 2020, Relationships Among Lymphocyte Subsets, Cytokines, and the Pulmonary Inflammation Index in Coronavirus (COVID-19) Infected Patients. British Journal of Haematology, 189(3), 428–437, https://doi.org/10.1111/bjh.16659

[44].  Tvarijonaviciute, A., Martinez-Lozano, N., Rios, R., Marcilla de Teruel, M. C., Garaulet, M., and Cerón, J. J., 2020, Saliva as a Non-Invasive Tool for Assessment of Metabolic and Inflammatory Biomarkers in Children. Clinical Nutrition (Edinburgh, Scotland), 39(8), 2471–2478, https://doi.org/10.1016/j.clnu.2019.10.034

[45].  Parra, M. D., Tecles, F., Martínez-Subiela, S., & Cerón, J. J., 2005, C-Reactive Protein Measurement in Canine Saliva. Official Publication of the American Association of Veterinary Laboratory Diagnosticians. Journal of Veterinary Diagnostic Investigation Inc, 17(2), 139–144. https://doi.org/10.1177/104063870501700207

[46].  Chen, L., Zhao, J., Peng, J., Li, X., Deng, X., Geng, Z., Shen, Z., Guo, F., Zhang, Q., Jin, Y., Wang, L., & Wang, S., 2020, Detection of SARS-CoV-2 in Saliva and Characterization of Oral Symptoms in COVID-19 patients. Cell proliferation, 53(12), e12923. https://doi.org/10.1111/cpr.12923

[47].  Williams, E., Bond, K., Zhang, B., Putland, M., & Williamson, D. A., 2020, Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2. Journal of Clinical Microbiology, 58(8), e00776-20. https://doi.org/10.1128/JCM.00776-20

[48].  Sagar, S., Ramani, P., Moses, S., Gheena, S., & Selvaraj, J., 2024, Correlation of Salivary Cytokine IL-17A and 1,25 Dihydroxycholecalciferol in Patients Undergoing Orthodontic Treatment. Odontology, 112(3), 966–975. https://doi.org/10.1007/s10266-023-00890-1

[49].   Alam, M. K., Zaman, M. U., Alqhtani, N. R., Alqahtani, A. S., Alqahtani, F., Cicciù, M., & Minervini, G., 2024, Salivary Biomarkers and Temporomandibular Disorders: A Systematic Review conducted according to PRISMA guidelines and the Cochrane Handbook for Systematic Reviews of Interventions. Journal of oral rehabilitation, 51(2), 416–426. https://doi.org/10.1111/joor.13589

[50].   Kritika, S., Mahalaxmi, S., Srinivasan, N., & Krithikadatta, J., 2023, Deciphering the Role of Saliva in COVID 19: A Global Cross-Sectional Study on the Knowledge, Awareness and Perception Among Dentists. BMC Oral Health,23(1), 424. https://doi.org/10.1186/s12903-023-03152-2

Abstract:

This research focuses on problem related to medical visit in the Mfoundi Division. It is aimed at measuring the extent and effect of the medical visit in medical and pharmaceutical practice in Yaoundé. A mixed, quantitative and qualitative study with descriptive and analytical goals was conducted. It involved a survey carried out over a period of five (5) months from August 1st, 2023 to January 31, 2024 in health facilities of the seven Yaoundé sub-divisions. Data was collected using a questionnaire (either online or physically) self-administered to 464 medical doctors (students, general practitioners, residents or specialists) met in consultation services and an interview guide administered to 12 community and hospital pharmacists. The data obtained was subject to manual and computer processing using SPSS 21, STATA 16 and Excell software. Results revealed that 36.2% of respondents received medical sales representatives (MSR) every day, 62.5% granted an interview to 5 or 10 MSR per week and almost all laid emphasis on the information of their products, on new products and the advantages of the product compared to competing products. The vast majority of respondents believed that relations with MSR were good, and 96.3% found that the medical visit was favorable to their activity. 92.3% of participants admitted to have changed their prescribing habits after the visit of a medical representative and 82.4% of their colleagues saw their prescriptions changed after an interview with a MSR.

References:

[1].   WHO, 1988, Ethical Criteria for Medicinal Drug Promotion. Geneva: WHO. (Accessed Aug 02, 2021).

[2].   [2] French Public Health Code, 2008, Article L5122-1, p.387 https://www.legifrance.gouv.fr/CSP/pdf/PP2008M.pdf.

[3].   Ventola, C. L., 2011, Direct-to-Consumer Pharmaceutical Advertising: Therapeutic or Toxic? 36 (10), 669-684.

[4].   Hailu, A. D., Workneh, B. D., Kahissay, M. H., 2021, Influence of Pharmaceutical Marketing Mix Strategies on Physicians' Prescribing Behaviors in Public and Private Hospitals, Dessie, Ethiopia: A Mixed Study Design. BMC Public Health, 21 (1) :65. doi: 10.1186/s12889-020-10063-2

[5].   LEEM - Les Entreprises Du Medicament En France, 2014, Bilan Economique - Edition 2014 [Internet]. [Cité 23 Août 2015]. Disponible sur : https://www.calameo.com/read/002049284aabedac20e30

[6].   Gagnon, M. A., Lexchin, J., 2008, The Cost of Pushing Pills: A New Estimate of Pharmaceutical Promotion Expenditures in the United States. PLoS Med, 5(1) : e1

[7].   Bras P. L., Ricordeau, P., Roussille, B., Saintoyant, V., 2007, L’information Des Medecins Generalistes Sur Le Medicament. IGAS; 247.

[8].   Benhaim J., 1987, Rôle et Importance De La Visite Medicale Et Des Delegues Medicaux Dans L’information Aupres Des Medecins. Th. : Pharm. : Nancy I ; 54.

[9].   Wazana A., 2000, Physicians and the Pharmaceutical Industry. JAMA, 283(3) :373

[10].  Lexchin J., 1989, Doctors and Detailers: Therapeutic Education or Pharmaceutical Promotion? Int J Health Serv, 19(4) :663–679.

[11].  Brett, A. S., Burr, W., Moloo, J., 2003, Are Gifts from Pharmaceutical Companies Ethically Problematic? : A Survey of Physicians. Arch Intern Med, 163(18) :2213

[12].  McCormick, B. B., Tomlinson, G., Brill-Edwards, P., Detsky, A. S., 2001, Effect of Restricting Contact Between Pharmaceutical Company Representatives and Internal Medicine Residents on Posttraining Attitudes and Behavior. JAMA, 286(16) :1994–1999.

[13].  Randall, M. L., Rosenbaum, J. R., Rohrbaugh, R. M., Rosenheck, R. A., 2005, Attitudes and Behaviors of Psychiatry Residents Toward Pharmaceutical Representatives Before and After an Educational Intervention. Acad Psychiatry, 29(1) :33–39.

[14].  R F., 2011, I nspection Générale Des Affaires Sociales Enquête Sur Le MEDIATOR [Internet]. [Cited 2023 May 14]. p. 261. Available from: https://www.igas.gouv.fr/IMG/pdf/RM2011- 001P.pdf

[15].  Santé Magazine. Vaccin Gardasil® Contre Le Cancer Du Col De L’utérus [Internet], 2023, [Cited 2023 May 14]. Available from: https://www.santemagazine.fr/sante/maladies/cancer/cancerdu-col-de-l-uterus/huit choses-a-savoir-sur-le-gardasil-levaccin-contre-le-cancer-du-col-de-luterus-194919

[16].  Ministere De La Sante Publique, 2013, Politique Pharmaceutique Nationale Du Cameroun. Minsanté. 2013 : 21-44.

[17].  Gnoumou, R., 2013, Etude De L’information Pharmacotherapeutique: Enquete Sur La Qualite Des Documents De Promotion Des Medicaments Distribues Par Les Visiteurs Medicaux Aux Prescripteurs Du Centre Hospitalier Universitaire Yalgado Ouedraogo. [Ouagadougou] : Université de Ouagadougou

[18].  Ibiassi, J., 2006, Enquete Sur La Qualite Des Publicites Pharmaceutiques Au Niveau D’une Officine Au Congo Brazzaville [Internet]. Medecine Tropicale. [Cited 2023 May 14]. p. 581–2. Available from: https://www.jle.com/fr/MedSanteTrop/2006/66.6/581-582 Enquête sur la qualité des publicités pharmaceutiques au niveau d%27une officine au Congo Brazzaville (Ibiassi).pdf

[19].  Mellouli, M., Bougmiza, I., Bargaoui, D., Zedini, C., El Ghardallou, M., Ajmi, T., 2016 Attitudes Et Pratiques Des Medecins Specialistes De La Region De Sousse (Tunisie) A L’égard De La Promotion Pharmaceutique. Revue D’épidemiologie Et De Sante Publique. Mars 2016; 64:S31.

[20].   Worldometer, 2020, (www.worldometers.info).

[21].  Rapport Stratégie Sectorielle De La Santé 2020-2030.

[22].  Inspection Generale Des Affaires Sociales (IGAS), 2007, L’information Des Medecins Generalistes Sur Le Medicament [Internet]. [Cited 2023 May 14]. Available from: https://62.210.98.10/IMG/pdf/Rapport_RM_2007-136P.pdf

[23].  Haute Autorité De La Sante (HAS), 2009, Charte De La Visite Medicale: Mise En Œuvre De La Procedure De Certification: Juillet 2006 – Octobre 2009  : Premier Bilan. [Internet]. 2009 [Cited 2023 May 14]. p. 54. Available from: https://www.hassante.fr/upload/docs/application/pdf/2009

[24].  Passeport Santé, 2011, Vaccination Contre L’hépatite B : Une Autre Controverse [Internet]. [Cited 2023 May 14]. Available from: https://www.passeportsante.net//fr/Actualites/Dossiers/ArticleComplementaire.aspx?doc=vaccination-contre-l-hepatite-B-uneautre-controverse_do

[25].  Waxman, H. A., 2005, The Lessons of Vioxx — Drug Safety and Sales. https://doi.org/101056/NEJMp058136 [Internet]. [cited 2023 May 14];352(25):2576–8. Available from: https://www.nejm.org/doi/full/10.1056/nejmp058136

[26].  Winncare 2019, Loi Anti-Cadeaux Et Transparence Des Liens D’interets Et Avantages [Internet], [Cited 2023 May 14]. p. 20. Available from: https://www.winncare.fr/pdf/espace_clinique/reglementaire/Leaflet_Reglementation_Loi_anticadeaux_Transparence_MAJ_27032019.pdf

[27].  Pittet, A. L., 2015, L’orientation De La Prescription Médicale Par L’industrie Pharmaceutique. Influence Des Visiteurs Medicaux Et Des Leaders D’opinion Sur La Prescription De Medecins Generalistes Et Psychiatres En Suisse Francophone. Thèse De Doctorat En Sciences De La Vie. Universite De Lausanne, Suisse.

[28].  Anonyme, 1999, Visite Médicale: Le Bilan Accablant Du Réseau D'observation De La Revue Prescrire. Rev Prescrire 19: 226-31.

[29].  Kareme, B. H., 2013, Gouvernance Hospitaliere Adaptative En Contexte Changeant: Etude Des Hopitaux De Bunia, Logo Et Katana En Republique Democratique Du Congo (Thesis): UCL.

[30].  Rowe, A. K., et al, 2005, How can we Achieve and Maintain High-Quality Performance of Health Wokers in Low-Resource Setting? The Lancet ;366:1026-35.

[31].  Khomeiran, R. T., Yekta, Z., Kiger, A., Ahmadi, F., 2006, Professional Competence: Factors Described by Nurses as Influencing their Development. International Nursing Review, 53(1) :66-72.

[32].  Demets, S., 2010, Evaluation De L'interet De La Visite Des Delegues Medicaux Au Cabinet Des Medecins Generalistes. S.L The. Méd. Lille 2.

[33].  Bras, P. L., et al., 2007, Information Des Généralistes Sur Le Médicament. S.L. Inspection Générale Des Affaires Sociales.

[34].  Radig, P., 2008, Une Prescription Sous Influence? Enquete D'opinion Sur La Visite Medicale Aupres Des Medecins Du Bas Rhin. S.L. The. Méd. Strasbourg 1.

[35].  Al-Areefi, M. A., Hassali, M. A., 2013, Physicians’ Perceptions of Medical Representative Visits in Yemen: A Qualitative Study. BMC Health Serv Res;13(1):331.

[36].  Sangho, F., Traoré Diop, A., Sangho, A., Sangho, O., Dianguina, S., Arama, D., et al., 2021, Effet De La Visite Des Delegues Medicaux Sur La Prescription Au CHU Du Point G. Mali Médical, XXXVI (3):6–11.

[37].  Lieb, K., Scheurich, A., 2014, Contact Between Doctors and the Pharmaceutical Industry, their Perceptions, and the Effects on Prescribing Habits. PLoS One, 2014 Oct 16 ;9(10).

[38].  Workneh, B. D., Gebrehiwot, M. G., Bayo, T. A., Gidey, M. T., Belay, Y. B., Tesfaye, D. M., et al., 2016, Influence of Medical Representatives on Prescribing Practices in Mekelle, Northern Ethiopia. PLoS One [Internet]. [cited 2023 May 12];11(6): e0156795. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pon e.0156795

[39].  Baron, S., Bourbon, L., 2012, Relations Entre Les Etudiants En Medecins Et L'industrie Pharmaceutique En France: Expositions Et Attitudes D'externes Et Internes Lyonnais. S.L The. Méd. Lyon 1, Lyon Sud.

[40].  Sagarin, S. B., et al., 2002, Dispelling the Illusion of Invulnerability: The Motivations and Mechanisms. Journal Of Personality and Social Psychology, https://www.niu.edu/user/tj0bjs1/papers/scrs02.pdf

[41].  Projet Collaboratif De L'organisation Mondiale De La Sante Et De L'action Internationale Pour La Sante., 2009, Comprendre La Promotion Pharmaceutique Et Y Repondre. [En ligne]. https://www.has_sante.fr/portail/upload/docs/application/pdf/2013,04/comprendre_la_promotion_pharmaceutique_et_y_repondre_- _un_manuel_pratique.pdf

Abstract:

Verruca vulgaris or common warts are benign epidermal growths caused by the Human Papillomavirus (HPV) often found on hands and legs. These lesions are self-limiting varying in number and size and can undergo malignant transformation into SCC. Basal cell carcinoma, the most common type of skin malignancy originates from the basal layer of skin typically in sunlight-exposed areas. And is diagnosed by doing an Edge wedge biopsy and Immunohistochemistry (IHC) tests. Here we present a case report of Basal Cell Carcinoma within a lesion of Verruca vulgaris over the dorsal aspect of the forearm which is an extremely rare presentation.

References:

[1].   Grayson, W., 2012, Infectious Diseases of the Skin. In E. Calonje, T., Brenn, A., Lazar, & P., Mckee (Eds.), Mckee’s Skin Pathology with Clinical Correlations (4th ed., pp. 760–895). Elsevier.

[2].   Cardoso, J. C., & Calonje, E., 2011, Cutaneous Manifestations of Human Papillomaviruses: A Review. Acta Dermatovenerologica Alpina, Pannonica et Adriatica, 20(3), 145–154.

[3].   Daugherty, M., & Byler, T., 2018, Genital Wart and Human Papillomavirus Prevalence in Men in the United States from Penile Swabs: Results from National Health and Nutrition Examination Surveys. Sexually Transmitted Diseases, 45(6), 412–416.

[4].   Noel, J. C., Detremmerie, O., Peny, M. O., et al. 1994, Transformation of Common Warts into Squamous Cell Carcinoma on Sun-Exposed Areas in an Immunosuppressed Patient. Dermatology, 189(3), 308–311.

[5].   Goette, D. K., 1980, Carcinoma in Situ in Verruca Vulgaris. International Journal of Dermatology, 19(2), 98–101.

[6].   Ergün, S. S., Su, O., & Büyükbabaný, N., 2004, Giant Verruca Vulgaris. Dermatologic Surgery, 30(3), 459–462.

[7].   Lai, K. K. H., Chan, E., & Ko, S. C., 2020, Combination of Squamous Cell Carcinoma and Basal Cell Carcinoma Arising from a Giant Verruca Vulgaris Involving the Eyelid. American Journal of Ophthalmology Case Reports, 21, 100858. https://doi.org/10.1016/j.ajoc.2020.100858

[8].   Sterling, J. C., Handfield-Jones, S., & Hudson, P. M., 2001, Guidelines for the Management of Cutaneous Warts. British Journal of Dermatology, 144, 4–11.

[9].   Lipke, M. M., 2006, An Armamentarium of Wart Treatments. Clinical Medicine & Research, 4(4), 273-293. https://doi.org/10.3121/cmr.4.4.273

[10].  Niculet, E., Craescu, M., Rebegea, L., Bobeica, C., Nastase, F., Lupasteanu, G., Stan, D. J., Chioncel, V., Anghel, L., Lungu, M., & Tatu, A. L., 2022, Basal Cell Carcinoma: Comprehensive Clinical and Histopathological Aspects, Novel Imaging Tools and Therapeutic Approaches (Review). Experimental and Therapeutic Medicine, 23(1), 60. https://doi.org/10.3892/etm.2021.10982

[11].  Cohen P. R., 2021, Verruca Vulgaris Occurring on a Tattoo: Case Report and Review of Tattoo-Associated Human Papillomavirus Infections. Cureus, 13(8), e17575. https://doi.org/10.7759/cureus.17575

[12].  Chatterjee, K., Roy, A., Ghosh, R., Barua, J. K., Halder, S., & Banerjee, G., 2017, The Bumpy Face of Che Guevara: An Interesting Case. Indian journal of dermatology, 62(6), 675. https://doi.org/10.4103/ijd.IJD_1_17

[13].  Paul, H., Akhtar, N., Zakaria, A. S. M., & Biswas, S. K., 2017, Generalized Verruca Vulgaris: A Case Report. 6, 29-32.

[14].  Pereira, R. de S., & Monteiro, N. M., 2022, Successful Treatment of Recalcitrant Non-genital Warts (Verruca vulgaris) with a Topical Solution Containing Two Antivirals and a Low Concentration of Salicylic Acid. Journal of Pharmaceutical Research International, 34(49B), 1–10. https://doi.org/10.9734/jpri/2022/v34i49B36426

[15].  Aldabagh, B., Angeles, J. G., Cardones, A. R., & Arron, S. T., 2013, Cutaneous Squamous Cell Carcinoma and Human Papillomavirus: Is there an Association? *Dermatologic Surgery*, 39(1), 1–23. https://doi.org/10.1111/j.1524-4725.2012.02558.x

[16].  Zaesim, A., Jackson, A. C., Lee, S. W., & Price, S. A., 2018, Squamous Cell Carcinoma Arising within Verruca Vulgaris on the Nipple. Case Reports in Dermatological Medicine, 2018, 1984593. https://doi.org/10.1155/2018/1984593

[17].  Kopelson, P. L., Nguyen, Q. H., & Moy, R. L., 1994, Verruca Vulgaris and Radiation Exposure are Associated with Squamous Cell Carcinoma of the Finger. The Journal of Dermatologic Surgery and Oncology, 20(1), 38-41. https://doi.org/10.1111/j.1524-4725.1994.tb03747.x

[18].  Ramakrishna, A., Donoghue, A. O., Mamatha, G. S., & Anuradha, M., 2014, Mucocutaneous Verruca Vulgaris: A Rare Presentation in an Immunocompetent Patient. Journal of Otology & Rhinology, 2015.

[19].  Al-Eitan, L. N., Alghamdi, M. A., Tarkhan, A. H., & Al-Qarqaz, F. A., 2020, Genome-Wide Identification of Methylated CpG Sites in Nongenital Cutaneous Warts. BMC Medical Genomics, 13(1), 100. https://doi.org/10.1186/s12920-020-00751-y

[20].  Zhu, P., Qi, R.-Q., Yang, Y., Huo, W., Zhang, Y., He, L., Wang, G., Xu, J., Zhang, F., Yang, R., Tu, P., Ma, L., Liu, Q., Li, Y., Gu, H., & Cheng, B., 2022, Clinical Guideline for the Diagnosis and Treatment of Cutaneous Warts. Journal of Evidence-Based Medicine, 15(3), 12494. https://doi.org/10.1111/jebm.12494

Abstract:

Colorectal cancer (CRC) is a prevalent and challenging type of cancer worldwide. Recent research has focused intensively on understanding the molecular and pathological mechanisms of CRC because of the poor prognosis associated with its treatment. The tumor microenvironment (TME) is crucial in tumor progression and has been the subject of extensive investigation. Metabolic reprogramming has become a central focus in cancer research, with numerous studies emphasizing its importance in CRC. Specifically, the reprogramming of fatty acids has been found to alter the energy and nutrient composition within the tumor microenvironment, affecting the complex interaction between immune cells, especially macrophages and T cells, and associated immune factors. This disruption can influence the tumor's ability to evade immune surveillance. Our in-depth analysis highlights the role of lipid metabolism processes in shaping the immune microenvironment of colorectal cancer tumors, revealing the regulatory impact of fatty acid metabolism on CRC development. The potential impact of this research on improving CRC treatment is significant, underscoring the importance of contribution to this field.

References:

[1].   Chen, X., Ma, Z., Yi, Z., Wu, E., Shang, Z., Tuo, B., and Liu, X., 2024, The Effects of Metabolism on the Immune Microenvironment in Colorectal Cancer, Cell Death Discovery, 10(1), 118. https://doi.org/10.1038/s41420-024-01865-z

[2].   Marcellinaro, R., Spoletini, D., Grieco, M., Avella, P., Cappuccio, M., Troiano, R., and Carlini, M., 2023, Colorectal Cancer: Current Updates and Future Perspectives. Journal of Clinical Medicine, 13(1), 40. https://doi.org/10.3390/jcm13010040

[3].   Chung, R. Y. N., Tsoi, K. K., Kyaw, M. H., Lui, A. R., Lai, F. T., and Sung, J. J. Y., 2019, A Population-Based Age-Period-Cohort Study of Colorectal Cancer Incidence Comparing Asia Against the West. Cancer Epidemiology, 59, 29-36. https://doi.org/10.1016/j.canep.2019.01.007

[4].   Zhang, S., Lv, K., Liu, Z., Zhao, R., and Li, F.,2024, Fatty Acid Metabolism of Immune Cells: A New Target of Tumour Immunotherapy. Cell Death Discovery, 10(1), 39. https://doi.org/10.1038/s41420-024-01807-9

[5].   Liberti, M. V., and Locasale, J. W., 2016, The Warburg Effect: How Does it Benefit Cancer Cells? Trends in Biochemical Sciences, 41(3), 211-218. https://doi.org/10.1016/j.tibs.2015.12.001

[6].   Akter, R., Awais, M., Boopathi, V., Ahn, J. C., Yang, D. C., Kang, S. C., and Jung, S. K., 2024, Inversion of the Warburg Effect: Unraveling the Metabolic Nexus between Obesity and Cancer. ACS Pharmacology & Translational Science, 7(3), 560-569. https://doi.org/10.1021/acsptsci.3c00301

[7].   Iranpanah ,A., Majnooni, M. B., Biganeh, H., Amirian, R., Rastegari-Pouyani, M., Filosa, R., Cheang, W. S, Fakhri, S., and Khan, H., 2024, Exploiting New Strategies in Combating Head and Neck Carcinoma: A Comprehensive Review on Phytochemical Approaches Passing Through PI3K/Akt/mTOR Signaling Pathway. Phytother Res, Jul;38(7):3736-3762. https://doi.org/10.1002/ptr.8228

[8].   Koundouros, N., and Poulogiannis, G., 2020, Reprogramming of Fatty Acid Metabolism in Cancer. British Journal of Cancer, 122(1), 4-22. https://doi.org/10.1038/s41416-019-0650-z

[9].   Shao, N., Qiu, H., Liu, J., Xiao, D., Zhao, J., Chen, C., and Xu, L., 2024, Targeting Lipid Metabolism of Macrophages: A New Strategy for Tumor Therapy. Journal of Advanced Research, S2090-1232. https://doi.org/10.1016/j.jare.2024.02.009

[10].  Wu, Y., Pu, X., Wang, X., and Xu, M., 2024, Reprogramming of Lipid Metabolism in the Tumor Microenvironment: A Strategy for Tumor Immunotherapy. Lipids in Health and Disease, 23(1), 35. https://doi.org/10.1186/s12944-024-02024-0

[11].  Chen, X., Chen, S., and Yu, D., 2020, Metabolic Reprogramming of Chemoresistant Cancer Cells and the Potential Significance of Metabolic Regulation in the Reversal of Cancer Chemoresistance. Metabolites, 10(7), 289. https://doi.org/10.3390/metabo10070289

[12].  Luo, Y., Wang, H., Liu, B., and Wei, J., 2022, Fatty Acid Metabolism and Cancer Immunotherapy. Current Oncology Reports, 24(5), 659-670. https://doi.org/10.1007/s11912-022-01223-1

[13].  Liang, X. H., Chen, X. Y., Yan, Y., Cheng, A. Y., Lin, J. Y., Jiang, Y. X., and Luan, X., 2024, Targeting Metabolism to Enhance Immunotherapy Within Tumor Microenvironment. Acta Pharmacologica Sinica, 1-12. https://doi.org/10.1038/s41401-024-01304-w

[14].  Jin, J., Byun, J. K., Choi, Y. K., and Park, K. G., 2023, Targeting Glutamine Metabolism as a Therapeutic Strategy for Cancer. Experimental & Molecular Medicine, 55(4), 706-715. https://doi.org/10.1038/s12276-023-00971-9

[15].  Pascual, G., Majem, B., and Benitah, S. A., 2024, Targeting Lipid Metabolism in Cancer Metastasis. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1879(1), 189051. https://doi.org/10.1016/j.bbcan.2023.189051

[16].  Wang, R., Yan, Q., Liu, X., and Wu, J., 2024. Unraveling Lipid Metabolism Reprogramming for Overcoming Drug Resistance in Melanoma. Biochemical Pharmacology, 116122. https://doi.org/10.1016/j.bcp.2024.116122

[17].  Gupta, A., Das, D., and Taneja, R., 2024, Targeting Dysregulated Lipid Metabolism in Cancer with Pharmacological Inhibitors. Cancers, 16(7), 1313. https://doi.org/10.3390/cancers16071313

[18].  Krauß, D., Fari, O., and Sibilia, M., 2022, Lipid Metabolism Interplay in CRC—An Update. Metabolites, 12(3), 213. https://doi.org/10.3390/metabo12030213

[19].  Endo, Y., Kanno, T., and Nakajima, T., 2022, Fatty Acid Metabolism in T-cell Function and Differentiation. International Immunology, 34(11), 579-587. https://doi.org/10.1093/intimm/dxac025

[20].  Liu, X. F., Shao, J. H., Liao, Y. T., Wang, L. N., Jia, Y., Dong, P. J., and Zhang, X., 2023, Regulation of Short-Chain Fatty Acids in the Immune System. Frontiers in Immunology, 14, 1186892. https://doi.org/10.3389/fimmu.2023.1186892

[21].  Kim, J. H., Kim, B. S., and Lee, S. K., 2020, Regulatory T Cells in Tumor Microenvironment and Approach for Anticancer Immunotherapy. Immune Network, 20(1). https://doi.org/10.4110/in.2020.20.e4

[22].  Zhang, Y., Kurupati, R., Liu, L., Zhou, X. Y., Zhang, G., Hudaihed, A., and Ertl, H. C., 2017, Enhancing CD8+ T Cell Fatty Acid Catabolism Within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma Immunotherapy. Cancer cell, 32(3), 377-391. https://doi.org/10.1016/j.ccell.2017.08.004

[23].  Sun, P., Zhang, X., Wang, R. J., Ma, Q. Y., Xu, L., Wang, Y., and Meng, L. H., 2021, PI3Kα Inhibitor CYH33 Triggers Antitumor Immunity in Murine Breast Cancer by Activating CD8+ T Cells and Promoting Fatty Acid Metabolism. Journal for Immunotherapy of Cancer, 9(8). https://doi.org/10.1136/jitc-2021-003093

[24].  Wang, Z., Little, N., Chen, J., Lambesis, K. T., Le, K. T., Han, W., and Lu, J., 2021, Immunogenic Camptothesome Nanovesicles Comprising Sphingomyelin-Derived Camptothecin Bilayers for Safe and Synergistic Cancer Immunochemotherapy. Nature Nanotechnology, 16(10), 1130-1140. https://doi.org/10.1038/s41565-021-00950-z

[25].  Wu, H., Han, Y., Rodriguez Sillke, Y., Deng, H., Siddiqui, S., Treese, C., and Glauben, R., 2019, Lipid Droplet‐Dependent Fatty Acid Metabolism Controls the Immune Suppressive Phenotype of Tumor‐Associated Macrophages. EMBO Molecular Medicine, 11(11), e10698. https://doi.org/10.15252/emmm.201910698

[26].  Düvel, K., Yecies, J. L., Menon, S., Raman, P., Lipovsky, A. I., Souza, A. L., and Manning, B. D., 2010, Activation of a Metabolic Gene Regulatory Network Downstream of mTOR Complex 1. Molecular cell, 39(2), 171-183. https://doi.org/10.1016/j.molcel.2010.06.022

[27].  Ma, X., Bi, E., Lu, Y., Su, P., Huang, C., Liu, L., and Yi, Q., 2019, Cholesterol Induces CD8+ T Cell Exhaustion in the Tumor Microenvironment. Cell Metabolism, 30(1), 143-156. https://doi.org/10.1016/j.cmet.2019.04.002

[28].  Ventura, A., Kirsch, D. G., McLaughlin, M. E., Tuveson, D. A., Grimm, J., Lintault, L., and Jacks, T., 2007, Restoration of p53 Function Leads to Tumour Regression in Vivo. Nature, 445(7128), 661-665. https://doi.org/10.1038/nature05541

[29].  Keerthivasan, S., Aghajani, K., Dose, M., Molinero, L., Khan, M. W., Venkateswaran, V., and Gounari, F., 2014, β-Catenin Promotes Colitis and Colon Cancer Through Imprinting of Proinflammatory Properties in T Cells. Science Translational Medicine, 6(225), 225ra28-225ra28. https://doi.org/10.1126/scitranslmed.3007607

[30].  Yu, F. X., Zhao, B., Panupinthu, N., Jewell, J. L., Lian, I., Wang, L. H., and Guan, K. L., 2012, Regulation of the Hippo-YAP Pathway by G-Protein-Coupled Receptor Signaling. Cell, 150(4), 780-791. https://doi.org/10.1016/j.cell.2012.06.037

[31].  Zhang, D., Shi, R., Xiang, W., Kang, X., Tang, B., Li, C., and Miao, H., 2020, The Agpat4/LPA Axis In Colorectal Cancer Cells Regulates Antitumor Responses Via p38/p65 Signaling in Macrophages. Signal Transduction and Targeted Therapy, 5(1), 24. https://doi.org/10.1038/s41392-020-0117-y

[32].  Chen, J., Zhu, H., Yin, Y., Jia, S., and Luo, X., 2022, Colorectal Cancer: Metabolic Interactions Reshape the Tumor Microenvironment. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1877(5), 188797. https://doi.org/10.1016/j.bbcan.2022.188797

[33].  Wang, Z., Cordova, L. E., Chalasani, P., and Lu, J.,2022, Camptothesome Potentiates PD-L1 Immune Checkpoint Blockade for Improved Metastatic Triple-Negative Breast Cancer Immunochemotherapy. Molecular Pharmaceutics, 19(12), 4665-4674. https://doi.org/10.1016/j.bbcan.2022.188797

Abstract:

The COVID-19 pandemic has significantly impacted global public health, with effective vaccine distribution being crucial to mitigating virus spread and reducing healthcare burdens. This study investigates determinants of COVID-19 vaccine uptake among adults in Kogi State, Nigeria, using the Health Belief Model (HBM) and the Theory of Planned Behavior (TPB). A cross-sectional survey of 484 participants selected through multistage sampling involved a structured questionnaire covering demographic information and HBM and TPB constructs. Results show that the age category (35-44) and senatorial zone (Kogi East) significantly predict vaccine uptake, with individuals aged 35-44 years being 2.5 times more likely to get vaccinated (OR = 2.5, p < 0.05). Perceived behavioral control significantly influences vaccine uptake (Β = -0.112, p = 0.018), while perceived subjective norm (β = 0.038, p = 0.41) and behavioral intention (Β = 0.039, p = 0.423) have minimal impact. The HBM constructs, including perceived severity (Β = 0.062, p = 0.027), susceptibility (Β = 0.08, p < 0.001), barriers, and cues to action, provided a significant fit. However, the combined TPB model did not significantly explain the variability in vaccine uptake (F-_statistic = 2.025, p = 0.109). The study underscores the necessity for targeted public health interventions and longitudinal research to enhance vaccine uptake and provides insights for effective public health strategies and educational campaigns in Kogi State and similar contexts.

References:

[1].   Egbe, K. A., Lerum, N. I., Unah, U. V., Ike, A. C., Odoh, C. K., Egbe, F. A., & Ekwom, E. S., 2022, The Biology of SARS-CoV-2 and Epidemiological Analysis of COVID-19 Pandemic in Nigeria. Journal of Infection in Developing Countries, 16(2), 252–257. Retrieved from https://pubmed.ncbi.nlm.nih.gov/35298418/.

[2].   Bhargava, R., Ghiloria, A., Kapoor, P., Dawar, S., Yadav, N. R., & Jain, M., 2021, Covid-19 Vaccination A Saviour from Pandemic. Journal of Evolution of Medical and Dental Sciences, 10(38), 3424+ Retrieved from https://go.gale.com/ps/i.do?id=GALE%7CA677900963&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=22784748&p=HRCA&sw=w&userGroupName=anon%7E91b4594c&aty=open-web-entry.

[3].   Saxena, M., Patil, N., Sareen, M., & Tyagi, N., 2021, Promising a Safe Futuristic Hope to COVID-19 Pandemic Through Vaccination: A Review of Different Vaccines. Acta Scientific Dental Sciences, 5(5), 118-127. https://doi.org/10.31080/ASDS.2021.05.1176 Retrieved from https://actascientific.com/ASDS/ASDS-05-1176.php.

[4].   Eroglu, B., Nuwarda, R. F., Ramzan, I., & Kayser, V, 2021, A Narrative Review of COVID-19 Vaccines. Vaccines, 10(1), 62. https://doi.org/10.3390/vaccines10010062 Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779282/.

[5].   Hussain, A., Yang, H., Zhang, M., Liu, Q., Alotaibi, G., Irfan, M., He, H., Chang, J., Liang, X. J., Weng, Y., & Huang, Y., 2022, mRNA Vaccines for COVID-19 and Diverse Diseases. Journal of Controlled Release: Official Journal of the Controlled Release Society, 345, 314–333. https://doi.org/10.1016/j.jconrel.2022.03.032. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935967/.

[6].   Ogunniyi, T. J., Rufai, B. O., Uketeh, S. N., Turzin, J. K., Oyinloye, E. A., & Effiong, F. B., 2023, Two Years of COVID-19 Vaccination in Nigeria: A Review of the Current Situation of the Pandemic: A Literature Review. Annals of Medicine and Surgery (2012), 85(11), 5528–5532. https://doi.org/10.1097/MS9.0000000000001310. Retrieved from https://pubmed.ncbi.nlm.nih.gov/37915653/.

[7].   Leadership, (2022, June 7), COVID-19: NPHCDA Partners With Community Pharmacists to Boost Vaccination. Retrieved from https://leadership.ng/covid-19-nphcda-partners-community-pharmacists/.

[8].   NPHCDA, (2022, December 14), Daily Call-In Data Analysis of COVID-19 Vaccinations. Available from the National Primary Health Care Development Agency.

[9].   NPHCDA, 2023, Unpublished COVID-19 Call-In Data Analysis, June 2023. Data Collected by the National Primary Health Care Development Agency from Nationwide Call-in Centers, Nigeria. Available from the National Primary Health Care Development Agency.

[10].           Kalam, M. A., Davis, T. P., Jr, Shano, S., Uddin, M. N., Islam, M. A., Kanwagi, R., Islam, A., Hassan, M. M., & Larson, H. J., 2021, Exploring the Behavioral Determinants of COVID-19 Vaccine Acceptance Among an Urban Population in Bangladesh: Implications for Behavior Change Interventions. PloS one, 16(8), e0256496. https://doi.org/10.1371/journal.pone.0256496. Retrieved from https://pubmed.ncbi.nlm.nih.gov/34424913/.

[11].           Rosenstock, I. M., 1974, The Health Belief Model and Preventive Health Behavior. Health Education Monographs, 2(4), 354-386. https://doi.org/10.1177/109019817400200405 Retrieved from https://journals.sagepub.com/doi/abs/10.1177/109019817400200405.

[12].           Ajzen, I., 1991, The Theory of Planned Behavior. Organizational Behavior and Human Decision Processes, 50(2), 179-211. https://doi.org/10.1016/0749-5978(91)90020-thttps://www.sciencedirect.com/science/article/abs/pii/074959789190020T.

[13].           Edafe, E. A., & Okoro, T. E., 2022, Factors Associated with COVID-19 Vaccine Hesitancy Among Residents of a Semi-Urban Setting in Bayelsa State, Nigeria. Saudi Journal of Medical and Pharmaceutical Sciences, 8(7), 327–334. Retrieved from https://www.researchgate.net/publication/362050844_Factors_Associated_with_COVID_19_Vaccine_Hesitancy_among_Residents_of_a_Semi-Urban_Setting_in_Bayelsa_State_Nigeria.

[14].           Olawade, D. B., Wada, O. Z., Odetayo, A., Akeju, O. O., Asaolu, F. T., & Owojori, G. O., 2022, COVID-19 Vaccine Hesitancy Among Nigerian Youths: Case Study of Students in Southwestern Nigeria. Journal of Education and Health Promotion, 11, 244. https://doi.org/10.4103/jehp.jehp_1756_21. Retrieved from https://pubmed.ncbi.nlm.nih.gov/36177416/

[15].           Olu-Abiodun, O., Abiodun, O., Okafor, N., 2022, COVID-19 Vaccination in Nigeria: A Rapid Review of Vaccine Acceptance Rate and the Associated Factors. PLoS ONE 17(5): e0267691. https://doi.org/10.1371/journal.pone.0267691. Retrieved from https://pubmed.ncbi.nlm.nih.gov/35544545/.

[16].           Erubami, J., Okpeki, P., Ohaja, E., Anorue, U., & Ezugwu, M., 2022, Beyond Health Belief: Modeling the Predictors of Covid-19 Vaccine Uptake Among Social Media Users in Nigeria. Studies in Media and Communication, 10(2), 39–53. https://doi.org/10.11114/smc.v10i2.5600. Received from https://www.researchgate.net/publication/361275922_Beyond_Health_Belief_Modeling_the_Predictors_of_COVID-19_Vaccine_Uptake_among_Social_Media_Users_in_Nigeria.

[17].           Li, P., Wong, H., Alias, H., Wong, P. F., Lee, H. Y., & AbuBakar, S., 2020, The Use of The Health Belief Model to Assess Predictors of Intent to Receive The COVID-19 Vaccine and Willingness to pay. Human Vaccines & Immunotherapeutics, 16(9), 2204-2214. https://doi.org/10.1080/21645515.2020.1790279.

[18].           Alshagrawi S. S., 2023, Examining Health Sciences Students' Intention to Uptake COVID-19 Vaccination Using the Theory of Planned Behavior. Human Vaccines & Immunotherapeutics, 19(2), 2256044. https://doi.org/10.1080/21645515.2023.2256044.

[19].           Lau, E. Y. H., Li, J. B., & Chan, D. K. C.; 2023, Beyond Intention: Predicting Children’s COVID-19 Vaccine Uptake Using the Theory of Planned Behavior. Human Vaccines & Immunotherapeutics, 19(2). https://doi.org/10.1080/21645515.2023.2260530.

[20].           Zampetakis, L. A., & Melas, C., 2021, The Health Belief Model Predicts Vaccination Intentions Against COVID-19: A Survey Experiment Approach. Applied psychology. Health and well-being, 13(2), 469–484. https://doi.org/10.1111/aphw.12262.

[21].           Zakar, R., Momina, A. U., Shahzad, R., Shahzad, S., Hayee, M., Zakar, M. Z., & Fischer, F., 2022, COVID-19 Vaccination Acceptance in the Context of the Health Belief Model: Comparative Cross-Sectional Study in Punjab, Pakistan. International Journal Of Environmental Research And Public Health, 19(19), 12892. https://doi.org/10.3390/ijerph191912892. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9566713/.

[22].           Rasaq, A. K., A, O. O., Kayode, A. S., Adeyanju, A. T., Sylvanus, E. D., Michael, I. T., Adebayo, A. M., Oyemomi, I. A., Adedayo, S. T., Cosmas, A. C., Adewumi, B., Olayinka, O. T., & Oladipupo, A. O., 2023, Perception And Willingness to the Uptake of Covid-19 Vaccine Among Household-Heads in a Rural Community of South-Western Nigeria. African Journal of Infectious Diseases, 17(2), 1–8. https://doi.org/10.21010/Ajidv17i2.1.

[23].           Shmueli, L., 2021, Predicting Intention to Receive Covid-19 Vaccine Among the General Population Using the Health Belief Model and the Theory of Planned Behavior Model. BMC Public Health, 21(804). https://doi.org/10.1186/s12889-021-10816-7.

[24].           Chu, H., & Liu, S., 2021, Integrating Health Behavior Theories to Predict American's Intention to Receive a COVID-19 Vaccine. Patient Education and Counseling, 104(8), 1878–1886. https://doi.org/10.1016/j.pec.2021.02.031

[25].           Naing, L., Winn, T., & Nordin, R., 2006, Practical Issues in Calculating the Sample Size for Prevalence Studies. Archives of Orofacial Sciences, 1, 9-14. Retreived from https://www.researchgate.net/publication/45459002_Pratical_Issues_in_Calculating_the_Sample_Size_for_Prevalence_Studies.

[26].           Oche, M. O., Adamu, H., Musa, Y., Garba, I. H., Mohammad, A., Danmadami, H., Ijapa, A., Wali, A., Fansuri, H., Yusuf, F., Muhammad, H., Abba, M., & Aji, M., 2022, Perception and Willingness to Accept Covid-19 Vaccines: A Cross-Sectional Survey of the General Population of Sokoto State, Nigeria. PLOS ONE, 17(12), e0278332. https://doi.org/10.1371/journal.pone.0278332.

[27].           Naidoo, D., Meyer-Weitz, A., & Govender, K., 2023, Factors Influencing the Intention and Uptake of COVID-19 Vaccines on the African Continent: A Scoping Review. Vaccines, 11(4), 873. https://doi.org/10.3390/vaccines11040873. Retrieved from https://pmc.ncbi.nlm.nih.gov/articles/PMC10146577/.

[28].           Martin, C. S., Wong, E. L. Y., Wong, J., Huang, A. W. L., Cheung, K. L., Law, M. K. C., Chong, R. W. Y., Ng, C. K. C., Lai, S. S. B., Lau, J., Chen, Z., & Chan, P. K. S., 2021, Acceptance of the COVID-19 Vaccine Based on the Health Belief Model: A Population-Based Survey in Hong Kong. Vaccine, 39(7), 1148-1156. https://doi.org/10.1016/j.vaccine.2020.12.083. Retrieved from https://pubmed.ncbi.nlm.nih.gov/33461834/.

[29].           Trepanowski, R., & Drążkowski, D., 2022, Data on COVID-19 Vaccination Intention and its Predictors in Poland. Data in Brief, 42, 108227. https://doi.org/10.1016/j.dib.2022.108227. Retrieved from https://pubmed.ncbi.nlm.nih.gov/35530365/.

[30].           Limbu, Y. B., Gautam, R. K., & Pham, L. (2022). The Health Belief Model Applied to COVID-19 Vaccine Hesitancy: A Systematic Review. Vaccines, 10(6), 973. https://doi.org/10.3390/vaccines10060973.

[31].           Kim, S., & Lee, S.,2023, Health Belief Model Factors and Personal Characteristics Associated With COVID-19 Vaccination Behaviors in Adults. Computers, Informatics, Nursing: CIN, 41(10), 771–779. https://doi.org/10.1097/CIN.0000000000001038.

[32].           Apuke, O. D., & Tunca, E. A., 2023, Modelling the Factors that Predict the Intention to Take COVID-19 Vaccine in Nigeria. Journal of Asian and African Studies, 58(5), 664-680. https://doi.org/10.1177/00219096211069642.

[33].           Short, M. B., Marek, R. J., Knight, C. F., & Kusters, I. S., 2022, Understanding Factors Associated with Intent to Receive the Covid-19 Vaccine. Families, Systems & Health: The Journal of Collaborative Family Healthcare, 40(2), 160–170. https://doi.org/10.1037/fsh0000664.

[34].           Zahra, K., Kouhpeikar, H., Rahaei, Z., Zareipour, M., Dashti, S., & Gholamian, M. (2023). The Predictors of the Intention to Receive Covid-19 Vaccine Using the Health Belief Model and Theory of Planned Behavior in South Khorasan Province. Journal of Education and Health Promotion, https://doi.org/10.4103/jehp.jehp_1480_22.

[35].           Wong, L. P., Alias, H., Wong, P. F., Lee, H. Y., & AbuBakar, S., 2020, The Use of the Health Belief Model to Assess Predictors of Intent to Receive The COVID-19 Vaccine and Willingness to Pay. Human Vaccines & Immunotherapeutics, 16(9), 2204–2214. https://doi.org/10.1080/21645515.2020.1790279.

[36].           [36]         Steffens, M. S., Bullivant, B., Bolsewicz, K., King, C., & Beard, F., 2022, Factors Influencing Covid-19 Vaccine Acceptance in High-Income Countries Prior to Vaccine Approval and Rollout: A Narrative Review. International Journal of Public Health, 67, 1604221. https://doi.org/10.3389/ijph.2022.1604221

[37].           Harapan, H., Wagner, A. L., Yufika, A., Winardi, W., Anwar, S., Gan, A. K., Setiawan, A. M., Rajamoorthy, Y., Sofyan, H., & Mudatsir, M., 2020, Acceptance of a COVID-19 Vaccine in Southeast Asia: A Cross-Sectional Study in Indonesia. Frontiers in Public Health, 8, 381. https://doi.org/10.3389/fpubh.2020.00381. Retrieved from https://pubmed.ncbi.nlm.nih.gov/32760691/

[38].           Reiter, P. L., Pennell, M. L., & Katz, M. L., 2020, Acceptability of a COVID-19 Vaccine Among Adults in the United States: How Many People Would Get Vaccinated? Vaccine, 38(42), 6500–6507. https://doi.org/10.1016/j.vaccine.2020.08.043. Retrieved from https://pubmed.ncbi.nlm.nih.gov/32863069/.

[39].           Lee, J. J., Kang, K. A., Wang, M. P., Zhao, S. Z., Wong, J. Y. H., O'Connor, S., Yang, S. C., & Shin, S., 2020, Associations Between COVID-19 Misinformation Exposure and Belief With COVID-19 Knowledge and Preventive Behaviors: Cross-Sectional Online Study. Journal of Medical Internet Research, 22(11), e22205. https://doi.org/10.2196/22205. Retrieved from https://pubmed.ncbi.nlm.nih.gov/33048825/.

[40].           Yakasai, H. B., Lasisi, M. D., Uwha, E. P., & others., 2023, Assessment of Intention, Acceptance, and Hesitancy of COVID-19 Vaccination in Nigeria: A Systematic Review and Meta-Analysis. SSRN. https://doi.org/10.2139/ssrn.4579879. Retrieved from https://europepmc.org/article/PPR/PPR729877.

[41].           Joe-Ikechebelu, N. N., Umeh, U. M., Eleje, G. U., Igbodike, E. P., Ogbuefi, E. O., Akanwa, A. O., Echendu, S. T., Ngene, W. O., Okpala, A., Okolo, O. C., Okechukwu, C. E., Akabuike, J. C., Agu, H. O., Okpala, V. O., Nwazor, O. C., Nnedum, A., Esimone, C., Agwaniru, H. N., Ezeabasili, E. I., & Joe-Ikechebelu, B. B., 2023,  Acceptability Rate and Barriers to Covid-19 Vaccination Among Healthcare Workers in Chukwuemeka Odumegwu Ojukwu University Teaching Hospital, Amaku-Awka, Nigeria. Therapeutic Advances in Infectious Disease, 10, 20499361231174776. https://doi.org/10.1177/20499361231174776.

[42].           Adebowale, O. O., Adenubi, O. T.; Adesokan, H. K., Oloye, A. A., Bankole, N. O., Fadipe, O. E., Ayo-Ajayi, P. O., Akinloye, O. A., 2022, Sociodemographic Predictors Associated with COVID-19 Vaccine Acceptance Among Veterinary Professionals in Nigeria.Sokoto J.Vet. Sci.,20, 134–140. Retrieved from https://gphandlahdpffmccakmbngmbjnjiiahp/http://sokvetjournal.net/images/PDF/Vol_20_2/8.%20Adebowale%20et%20al.pdf.

[43].           ames, B. C., Ede, S. S., Aroh, C. M., Okoh, C. F., Kanokwan, C., Rasip, M. L., & Enbeyle, W., 2022, Attitudes And Perceptions Of Nigerians Regarding Receiving COVID-19 Vaccines: An Online Cross-Sectional Study. The Pan African Medical Journal, 41, 247. https://doi.org/10.11604/pamj.2022.41.247.33286.

[44].           Larson, H. J., Jarrett, C., Schulz, W. S., Chaudhuri, M., Zhou, Y., Dube, E., Schuster, M., MacDonald, N. E., Wilson, R., 2015, SAGE Working Group on Vaccine Hesitancy., Measuring Vaccine Hesitancy: The Development of a Survey Tool. Vaccine, 33(34), 4165–4175. https://doi.org/10.1016/j.vaccine.2015.04.037.

[45].           horpe, A., Fagerlin, A., Drews, F. A., Shoemaker, H., Brecha, F. S., & Scherer, L. D., 2024, Predictors of Covid-19 Vaccine Uptake: An Online Three-Wave Survey Study of US Adults. BMC Infectious Diseases, 24(1), 304. https://doi.org/10.1186/s12879-024-09148-9. Retrieve from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936026/.

[46].           Mondal, P., Sinharoy, A., & Su, L., 2021, Sociodemographic Predictors of Covid-19 Vaccine Acceptance: A Nationwide US-Based Survey Study. Public Health, 198, 252–259. https://doi.org/10.1016/j.puhe.2021.07.028 Retrieved from https://pubmed.ncbi.nlm.nih.gov/34492505/.

[47]. Fan, C. W., Chen, I. H., Ko, N. Y., Yen, C. F., Lin, C. Y., Griffiths, M. D., & Pakpour, A. H., 2021, Extended Theory of Planned Behavior in Explaining the Intention to Covid-19 Vaccination Uptake Among Mainland Chinese University Students: An Online Survey Study. Human Vaccines & Immunotherapeutics, 17(10), 3413–3420. https://doi.org/10.1080/21645515.2021.1933687.

Abstract:

Otomycosis is one of the most prevalent fungal diseases of the external auditory canal in the tropical and sub-tropical regions of the world. Numerous environmental and host factors can put a person at risk for otomycosis. This cross-sectional study was undertaken over a one-year period at a tertiary care hospital, in South India after approval from Institutional Human Ethics Committee (IHEC), as there is limited data available on the clinical-mycological profile and its association with various risk factors in our region in the recent past. 90 ear swabs from clinically diagnosed otomycosis patients were sent to the laboratory for mycological examination from the Ear, Nose, and Throat (ENT) out-patient Department. Their clinical data was evaluated using a self-administered questionnaire. Mycological examination yielded 63 fungal isolates with Aspergillus niger as the predominant organism followed by other fungi; common risk factors identified were diabetes mellitus (92%), CSOM patients on steroid drops (91.6%), swimming (80%), CSOM patients on antibiotic drops (75%) with a significant association of p<0.05 between the above-mentioned risk factors and otomycosis. The current study highlights that proper identification of the causative agents is mandatory to prevent complications and recurrences, even when the patients' otoscopic findings and clinical presentations strongly suggest a fungal infection. Comparable with other superficial fungal infections, otomycosis does not represent a threat to life. However, prompt microbiological identification are essential for timely and effective therapy to avert major issues from otomycosis.

References:

[1].   Ibekwe, T. S., & Nwaorgu, O. G., 2011, Classification and Management Challenges of Otitis Media in a Resource-Poor Country. Nigerian journal of clinical practice, 14(3), 262-262. https://doi.org/10.4103/1119-3077.86764

[2].   Agarwal, P., & Devi, L. S., 2017, Otomycosis in a Rural Community Attending a Tertiary Care Hospital: Assessment of Risk Factors and Identification of Fungal and Bacterial Agents. Journal of Clinical and Diagnostic Research: JCDR, 11(6): DC14-DC18. https://doi.org/10.7860/JCDR/2017/25865.10068

[3].    Mofatteh, M. R., Naseripour Yazdi, Z., Yousefi, M., Namaei, M.H., 2018, Comparison of the Recovery Rate of Otomycosis Using Betadine and Clotrimazole Topical Treatment. Brazilian Journal of Otorhinolaryngology, 84(4):404–409. https://doi.org/10.1016/j.bjorl.2017.04.004

[4].   Ho, T., Vrabec, J. T., Yoo, D., & Coker, N. J., 2006, Otomycosis: clinical features and treatment implications. Otolaryngology-Head and Neck Surgery: Official Journal of American Academy of Otolaryngology-Head and Neck Surgery, 135(5), 787–791. https://doi.org/10.1016/j.otohns.2006.07.008

[5].   Malcolm, T. R., & Chin‐Hong, P. V., 2013, Endemic Mycoses in Immunocompromised Hosts. Current Infectious Diseases Reports, 15(6), 536-543. https://doi.org/10.1007/s11908-013-0387-4

[6].   Tasić-Otašević, S., Golubović, M., Đenić, S., Ignjatović, A., Stalević, M., Momčilović, S., Bojanović, M., Arsić-Arsenijević, V., 2020, Species Distribution Patterns and Epidemiological Characteristics of Otomycosis in Southeastern Serbia. Journal de Mycologie Médicale, 30(3), 101011. https://doi.org/10.1016/j.mycmed.2020.101011

[7].   Pfaller, M. A., & Diekema, D. J., 2004, Rare and Emerging Opportunistic Fungal Pathogens: Concern for Resistance Beyond Candida Albicans and Aspergillus Fumigatus. Journal of Clinical Microbiology, 42(10), 4419-4431. https://doi.org/10.1128/jcm.42.10.4419-4431.2004

[8].   Ostrowski, V. B., & Wiet, R J., 1996, Pathologic Conditions of the External Ear and Auditory Canal. Postgraduate Medicine, 100(3), 223-237. https://doi.org/10.3810/pgm.1996.09.79

[9].   Fisher, M. C., Gurr, S. J., Cuomo, C. A., Blehert, D. S., Jin, H., Stukenbrock, E. H., Stajich, J. E., Kahmann, R., Boone, C., Denning, D. W., Gow, N. A. R., Klein, B. S., Kronstad, J. W., Sheppard, D. C., Taylor, J. W., Wright, G. D., Heitman, J., Casadevall, A., & Cowen, L. E., 2020, Threats Posed by the Fungal Kingdom to Humans, Wildlife, and Agriculture. Microbiology, 11(3), e00449-20. https://doi.org/10.1128/mBio.00449-20

[10].  Galimberti, R., Torre, A C., Baztán, M C., & Rodriguez-Chiappetta, F., 2012, Emerging Systemic Fungal Infections. Clinics in Dermatology, 30(6), 633-650. https://doi.org/10.1016/j.clindermatol.2012.01.011

[11].  Kamali Sarvestani, H., Seifi, A., Falahatinejad, M., & Mahmoudi, S., 2022, Black Aspergilli as Causes of Otomycosis in the Era of Molecular Diagnostics, a Mini-Review. Journal De Mycologie Medicale, 32(2), 101240. https://doi.org/10.1016/j.mycmed.2021.101240

[12].  Aneja, K.R., Sharma, C., Joshi, R., 2010, Fungal Infection of the Ear: A Common Problem in the North Eastern Part of Haryana. International Journal of Pediatric Otorhinolaryngology, 74:604–607. https://doi.org/10.1016/j.ijporl.2010.03.001

[13].  Buonafina-Paz, M. D., Santos, F. A., Leite-Andrade, M. C., Alves, A. I., Bezerra, J. D., Leal, M. C., Robert, E., Pape, P. L., Lima-Neto, R. G., & Neves, R. P., 2022, Otomycosis Caused by the Cryptic and Emerging Species Aspergillus Sydowii: Two Case Reports. Future Microbiology,17:1437–1443. https://doi.org/10.2217/fmb-2022-0137

[14].  Bojanović, M., Stalević, M., Arsić-Arsenijević, V., Ignjatović, A., Ranđelović, M., Golubović, M., Živković-Marinkov, E., Koraćević, G., Stamenković, B., Otašević, S., 2023, Etiology, Predisposing Factors, Clinical Features and Diagnostic Procedure of Otomycosis: A Literature Review. Journal of Fungi, 9(6):662. http://doi.org/10.3390/jof9060662

[15].  Bluestone, C D., & Klein, J O., 1999, Consultation with the Specialist: Chronic Suppurative Otitis Media. Pediatrics in Review, 20(8), 277-279. https://doi.org/10.1542/pir.20-8-277

[16].  Patricia, M. T., 2014, Bailey & Scott’s Diagnostic Microbiology. (Elsevier, St. Louis, MO). 10.1309/LM5JC0PH0OGGBSZZ

[17].  Odds, F. C., & Bernaerts, R., 1994, CHROMagar Candida, A New Differential Isolation Medium for Presumptive Identification of Clinically Important Candida Species. Journal of Clinical Microbiology, 32(8):1923–1929. https://doi.org/10.1128/jcm.32.8.1923-1929.1994

[18].  Yegane Mogadam, A., Asadi, M. A., Dehghani, R., Hooshyar, H., 2009, The Prevalence of Otomycosis in Kasham Iran During 2001-2003. Jundishapur J Microbiol, 2(1):18–21.

[19].  Asoegwu, C. N., Oladele, R. O., Kanu, O. O., Peters, R. F., & Nwawolo, C. C., 2023. Clinical and Microbiological Profile of Otomycosis in Lagos, Nigeria. The Nigerian Postgraduate Medical Journal,30(2), 132–136. https://doi.org/10.4103/npmj.npmj_9_23

[20].  Prasad, S. C., Kotigadde, S., Shekhar, M., Thada, N. D., Prabhu, P., D' Souza, T., & Prasad, K. C., 2014, Primary Otomycosis in the Indian Subcontinent: Predisposing Factors, Microbiology, and classification. International Journal of Microbiology, 2014, 636493. https://doi.org/10.1155/2014/636493

[21].  Hagiwara, S., Tamura, T., Satoh, K., Kamewada, H., Nakano, M., Shinden, S., Yamaguchi, H., & Makimura, K., 2019, The Molecular Identification and Antifungal Susceptibilities of Aspergillus Species Causing Otomycosis in Tochigi, Japan. Mycopathologia, 184(1), 13–21. https://doi.org/10.1007/s11046-018-0299-9

[22].  Onotai, L., & Osuji, A. E., 2017, Otomycosis: Management Challenges and Outcomes in a Resource Poor Country. The Nigerian Health Journal, 16(4), 219. https://doi.org/10.60787/tnhj.v16i4.260

[23].  Aggarwal, S. K., Jaiswal, K., 2019, Fungal Profile and its Characteristics in Patients of Otomycosis‑A Prospective Study. National Journal of Laboratory Medicine, 8(4):MO04-MO07.

[24].  Gupta, S., Mahajan, B., 2015, Prevalence and Demographical Profile of Patients Presenting with Otomycosis. JK Science, 17:138‑42.

[25].  Ameye, S. A., Adeyemo, A., Eziyi, J. A., Amusa, Y. B., 2018, Clinical Profile of Otomycosis in a Sub‑Saharan African Tertiary Health Center. An International Journal of Otorhinolaryngology Clinics, 10:52‑5. http://doi.org/10.5005/jp-journals-10003-1290

[26].  Howlader, A., Nagarajan, P., Ragunathan, L., 2022, Mycological Profile in Otomycosis Patients and their Drug Sensitivity: A Cross-sectional Study at Union Territory of Puducherry, India, Journal of Clinical And Diagnostic Research, 16(10), DC11-DC15. https://www.doi.org/10.7860/JCDR/2022/57715/17073

[27].  Ashopa,V., Verma,U., Nareda, P., Gupta, E., prakash, P., 2020, Assessment of Risk Factors and Microbial Profile of Otomycosis in Patients Attending Tertiary Level Hospital of Western Rajasthan, India, Journal of clinical and diagnostic research.14(2),DC01-DC04. https://www.doi.org/10.7860/JCDR/2020/43153/13477

[28].  Taneja, M. K., 1998, Acute Otitis Media. Indian Journal of Otology, 4(4):161-164.

[29].  Mawson, S. R., and Ludman, H., 1979, In:Diseases of the Ear: A Textbook of Otology. (Chicago Yearbook Medical Publishing, pp 328-30).

[30].   Kunelskaya, V. Y. A., 1969, Significance of Fungal Flora in Chronic Suppurative Otitis Media. Vestn Otorinolaringol (Rus), 31(2):28–32.

[31].  Viswanatha, B., & Naseeruddin, K., 2011, Fungal Infections of the Ear in Immunocompromised Host: A Review. Mediterranean Journal of Hematology and Infectious Diseases, 3(1): e2011003. http://doi.org/10.4084/MJHID.2011.003

[32].  Anwar, K., & Gohar, M. S., 2014, Otomycosis; Clinical Features, Predisposing Factors and Treatment Implications. Pakistan Journal of Medical Sciences, 30(3):564–567. https://doi.org/10.12669/pjms.303.4106

[33].  Pontes, Z. B., Silva, A. D., Lima, E.deO., Guerra, M.deH., Oliveira, N. M., Carvalho, M.deF., & Guerra, F. S., 2009, Otomycosis: A Retrospective Study. Brazilian Journal of Otorhinolaryngology,75(3), 367–370. https://doi.org/10.1016/S1808-8694(15)30653-4

[34].  Padma, R., Mrunal, P., Ketki, P., Nidhi, A., 2023, Otitis Media and its Associated Risk Factors: An Investigation into Patients’ Knowledge, Attitudes, Practices, and Information Sources at a Hospital Setting. MGM Journal of Medical Sciences, 10(3): p 540-547.https://doi.org/10.4103/mgmj.mgmj_127_23

[35].  Suresh, S., Suresh, S., & Sivasamy, S., 2021, Prevalence of Otomycosis in Patients with Cerumen Impaction due to Earphone Usage During COVID-19. Indonesian Journal of Medical Sciences and Public Health. 2(1), 28 – 39. https://doi.org/10.11594/ijmp.02.01.04

Abstract:

Despite the significant advances in modern medicine over the last few decades, plants continue to play an important role in health care. Antioxidant capabilities have been studied in a large range of medicinal plants. Natural antioxidants, whether in the form of raw extracts or chemical components, are extremely efficient in preventing oxidative stress-related damage. The objective of the study is to evaluate the synergistic antioxidant property of P.guajava and raw honey with Butylated hydroxytoluene as a standard comparison agent. The plant extract (P.guajava) was prepared by the cold infusion method. 1ml (1mg/ ml) of Guava leaf extract (GLE) was added to 1ml of honey and mixed well. 1ml of this sample mixture was taken and diluted with sterile distilled water to attain different concentrations of the sample such as 500 µg/ml,400 µg/ml, 300 µg/ml, 200 µg/ml and 100 µg/ml. The percentage of antioxidant activity (AA %) of each substance was assessed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. This assay was performed with different concentrations of P.guajava and raw honey combination. The test sample showed [42.8%] antioxidant activity at 500 µg/ml concentration whereas the standard agent showed 99.8% activity at 500 µg/ml. So, the standard extract yielded the greatest percentage, while the combination extract [Guava leaf and raw honey] yielded the lowest. From this study, it is evident that the antioxidant property of the test agent (P.guajava and raw honey) is comparatively less significant than the standard. Overall, the combination of guava leaves and raw honey proved effective in developing antioxidant capabilities. These parameters might be adjusted either directly as a solution to create rapid reactive oxygen species mitigation or indirectly to achieve a delayed, sustained antioxidant effect.

References:

[1].   Melo, C., Cornejal, N., Cruz, V., Alsaidi, S., Cruz Rodriguez, G., Gomez Ramirez, A., Sorel, V., Bonnaire, T., Zydowsky, T. M., Priano, C., & Fernandez Romero, J., 2020, Antioxidant Capacity and Antimicrobial Activity of Commercial Samples of Guava Leaves (Psidium Guajava). Journal of Medicinally Active Plants, 9(1), 2.

[2].   Sharifi-Rad, M., Anil Kumar, N. V., Zucca, P., Varoni, E. M., Dini, L., Panzarini, E., Rajkovic, J., TsouhFokou, P. V., Azzini, E., Peluso, I., & Prakash Mishra, A., 2020, Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Frontiers in Physiology, 11, 694.

[3].   Jamieson, S., Wallace, C. E., Das, N., Bhattacharyya, P., & Bishayee, A., 2021, Guava (Psidium Guajava L.): A Glorious Plant with Cancer Preventive and Therapeutic Potential. Critical Reviews in Food Science and Nutrition, 1-32.

[4].   Seo, J., Lee, S., Elam, M. L., Johnson, S. A., Kang, J., & Arjmandi, B. H., 2014, Study To Find The Best Extraction Solvent for use with Guava Leaves (Psidium Guajava L.) for High Antioxidant Efficacy. Food Science & Nutrition, 2(2), 174-180.

[5].   Ahmed, S., Sulaiman, S. A., Baig, A. A., Ibrahim, M., Liaqat, S., Fatima, S., Jabeen, S., Shamim, N., & Othman, N. H., 2018, Honey as a Potential Natural Antioxidant Medicine: An Insight into its Molecular Mechanisms of Action. Oxidative Medicine and Cellular Longevity, 2018.

[6].   Subba Rao, Ch., Arun Kumar, S., Jawed, F. A., et al. 2018, In Vitro Antioxidant Activity of Psidium Guajava Linn. By Using Ethanolic Extract Fraction of Leaves and Bark. Advances in Cell Science and Tissue Culture, 2(1), 12-1.

[7].   Shekhar, T. C., & Anju, G., 2014, Antioxidant Activity by DPPH Radical Scavenging Method of Ageratum Conyzoides Linn. Leaves. American Journal of Ethnomedicine, 1(4), 244-249.

[8].   Liu, R. H., 2003, Health Benefits of Fruit and Vegetables are from Additive and Synergistic Combinations of Phytochemicals. The American Journal of Clinical Nutrition, 78(3), 517S-520S.

[9].   Pham-Huy, L. A., He, H., & Pham-Huy, C., 2008, Free Radicals, Antioxidants in Disease and health. International Journal of Biomedical Science: IJBS, 4(2), 89.

[10].  Naseer, S., Hussain, S., Naeem, N., Pervaiz, M., & Rahman, M., 2018, The Phytochemistry and Medicinal Value of Psidium Guajava (Guava). Clinical Phytoscience, 4(1), 1-8.

[11].  Ranneh, Y., Akim, A. M., Hamid, H. A., Khazaai, H., Fadel, A., Zakaria, Z. A., Albujja, M., & Bakar, M. F., 2021, Honey and its Nutritional and Anti-Inflammatory Value. BMC Complementary Medicine and Therapies, 21(1), 1-7.

[12].  Yadav, A. R., Mohite, S. K., Rajput, M. D., Suryawanshi, V. S., Birajdar, R. M., & Patil, M. V., 2020, Antioxidant Activity of Psidium Guajava Leaf Extracts. Research Journal of Pharmaceutical Dosage Forms and Technology, 12(3), 159-161.

[13].  Ahmed, M., Khiati, B., Meslem, A., Aissat, S., & Djebli, N., 2014, Evaluation of Physicochemical and Antioxidant Properties of Raw Honey from Algeria. Journal of Microbial & Biochemical Technology, S4.

[14].  Oncho, D. A., Ejigu, M. C., & Urgessa, O. E., 2021, Phytochemical Constituent and Antimicrobial Properties of Guava Extracts of East Hararghe of Oromia, Ethiopia. Clinical Phytoscience, 7(1), 37.

[15].  Ravi Kumar, S., & Sridevi, S. 2020, "Psidium Guajava: A Review on its Antioxidant Properties and Health Benefits." Journal of Pharmacognosy and Phytochemistry, 9(5), 181-190.

[16].  Sharma, A., & Sharma, R. K., 2019, "Antioxidant Activity of Psidium Guajava Linn: A Review." Journal of Drug Delivery and Therapeutics, 9(2), 352-358.

[17].  Panda, S. K., & Kar, A., 2013, "Evaluation of the Antioxidant and Synergistic Activity of Different Plant Extracts in Combination with Synthetic Antioxidants." Journal of Food Science and Technology, 50(4), 795-803.

[18].  Wang, L., & Liu, T., 2017, "Synergistic Antioxidant Effect of Natural Plant Extracts: A Review." Journal of Food Quality, 2017, 1-10.

[19].  Finkel, T., & Holbrook, N. J., 2000, "Oxidants, Oxidative Stress and the Biology of Ageing." Nature, 408(6809), 239-247.

[20].  Pisoschi, A. M., & Negulescu, G. P., 2012, "Methods for Total Antioxidant Activity Determination: A Review." Biochemistry & Analytical Biochemistry, 1(3), 1-10.

[21].  Halliwell, B., & Gutteridge, J. M., 2015, Free Radicals in Biology and Medicine. Oxford University Press.

[22].  Sies, H., 2015, Oxidative Stress: A Concept in Redox Biology and Medicine. Redox Biology, 4, 180-183.

[23].  Brand-Williams, W., Cuvelier, M. E., & Berset, C., 1995, Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensmittel-Wissenschaft & Technologie, 28(1), 25-30.

[24].  Benzie, I. F. F., & Strain, J. J., 1996, The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239(1), 70-76.

[25].  Akinmoladun, I. A., & Adeyemi, O. S., 2009, Protective Effects of Psidium guajava Linn. (Myrtaceae) on Carbon Tetrachloride-Induced Hepatotoxicity in Rats. Journal of Medicinal Food, 12(1), 85-90.

[26].  Swamy, M. K., & Sinniah, U. R., 2015, A Review of the Phytochemistry and Pharmacology of Psidium Guajava (Guava). Bio.Med Research International, 2015, 1-10.

[27].  Yadav, P., & Agarwal, S., 2020, Assessment of Antioxidant Activity and Phytochemical Profile of Psidium Guajava Leaf Extracts. Journal of Pharmacognosy and Phytochemistry, 9(3), 76-82.

[28].  Crozier, A., Clifford, M. N., & Ashihara, H., 2006, Plant Secondary Metabolites: Occurrence, Structure and Role in the Human Diet. Blackwell Publishing.

[29].  Liu, R. H., 2013, Health Benefits of Fruits and Vegetables are from Additive and Synergistic Combinations of Phytochemicals. American Journal of Clinical Nutrition, 98(1), 126-135.

[30].  Ahmed, S., & Tanvir, E. M., 2017, Antioxidant Potential and Bioactive Compounds in Honey: A Comprehensive Review. Food Chemistry, 214, 633-642.

[31].  Bogdanov, S., & Martin, P., 2002, Honey: Composition and Properties. In Honey: A Comprehensive Review of its Chemistry and Health Benefits. Springer.

[32].  Lee, C. H., & Lee, J. S., 2010, Polyphenols in Honey: Quantification and Antioxidant Activity. Journal of Food Science, 75(4), 343-349.

[33].  Ali, M., & Ahmed, I., 2015, Evaluation of Antioxidant and Antimicrobial Properties of Guava (Psidium guajava) Leaf Extracts. Pharmacognosy Research, 7(2), 175-180.

[34].  Sharma, A., & Sharma, A. K., 2016, Comparative Evaluation of Antioxidant Activities of Honey and Guava Extracts. Journal of Medicinal Plants Studies, 4(1), 22-29.

[35].  Kaur, C., & Kapoor, H. C., 2001, Anti-Oxidant Activity and Total Phenolic Content of Some Indian Vegetables. International Journal of Food Science & Technology, 36(2), 171-177.

[36].  Singh, R., & Sharma, R., 2012, Synergistic Effects of Polyphenols on Antioxidant Capacity of Foods. Food Research International, 49(1), 55-63.

[37].  Gülçin, İ., 2020, Antioxidant Activity of Guava and Honey: A Comparative Study. Journal of Agricultural and Food Chemistry, 68(10), 2950-2956.

[38].  Visavadiya, N. P., & Nair, J., 2011, Beneficial Effects of Guava and Honey Combination on Cardiovascular Health. Cardiovascular Therapeutics, 29(5), 31

Abstract:

Oral cancer is a serious health concern requiring target-specific treatment strategies due to its high rates of morbidity and mortality. Early detection of cancer cells considerably improves prognosis and treatment outcomes. Recently, nanomaterials have opened a world of intriguing new possibilities for cancer diagnosis and therapy by providing creative ways to enhance patients' quality of life. Furthermore, modified nanoparticles use specially designed moieties to target cancer cells more precisely. Drug delivery methods could reduce side effects and significantly improve oral cancer treatment efficacy. In addition, polymer-based drug delivery methods are designed to release drugs gradually and in a controlled way. In addition, the combination therapy with a drug delivery system leads to a synergetic effect are also discussed.

References:

Abstract:

Oral squamous cell carcinoma is a multi-factorial disease and no single causative factor has been primarily held responsible. Its pathogenesis is a multistep process involving initiation, promotion and tumor progression. The tumor micro-environment, particularly the collagen characteristics, and mesenchymal cells like myofibroblasts are increasingly implicated. The present study aims to determine the myofibroblast distribution and stromal characteristics in different grades of oral squamous cell carcinoma. Total of 46 specimens of different grades of oral squamous cell carcinoma were selected from departmental archives and subjected to immunohistochemical evaluation of myofibroblasts using α-smooth muscle actin marker. Parallel sections were subjected to van gieson staining to determine the characteristics of the collagen of the tumor stroma. Statistical analysis was performed for comparison between the different grades of oral squamous cell carcinoma. 80% of squamous cell carcinoma specimens were positive for α-smooth muscle actin.  Poorly differentiated carcinoma specimens consistently demonstrated higher concentration of α-SMA positive myofibroblasts and a dense stroma. There was a positive correlation between myofibroblasts and the stromal density. The interesting feature was the higher concentration of myofibroblasts in the stroma away from the tumor islands the results indicate that the effect of the myofibroblasts on the tumor stroma may play a role in the fundamental cellular processes essential for tumor progression. It is well known that TGF-β dependent accumulation of collagen type 1 in tumor microenvironment is related to increased tumor invasiveness. Therefore, myofibroblasts may play a role in tumor growth and invasion.

References:

[1]. Abeloff, M. D., Armitage, J. O., Niederhuber, J. E., Kastan, M. B, McKenna, W. G., eds., 2008, Abeloff’s Clinical Oncology. 4th ed. Philadelphia, Pa: Elsevier Churchill Livingstone.

[2]. Wynder, E. L., Stellman, S. D., 1977, Comparative epidemiology of tobacco-related cancers. Cancer Res., 37, 4608-22.

[3]. De, Wever, O. and Mareel, M., 2003, Role of tissue stroma in cancer cell invasion. J. Pathol., 200, 429–44

[4]. Schiich, W., Lagac, R., Seemayer, T. A., 1982, Myofibroblastic stromal reaaction in retracted scirrhous carcinomas of the breast. Surg Gynecol and Obstet, 154,525-533.

[5]. Lagace, R., Grimaud, J. A., Schiirch, W., Seemayer, T. A., 1985, Stromal reaction in carcinomas of the breast and variations of collagenous matrix and structural glycoproteins. Virchows Arch [A], 408, 49-59

[6]. Bhawan, I., Bacchetta, C., Majno, G. A., 1979, Myofibroblastic tumor. Infantile digital fibroma (recurrent digital fibrous tumor of childhood). Am J PathoI, 94, 19-36

[7]. Tremblay, G., 1979, Stromal aspects of breast carcmoma. Exp Mol Pathol, 31, 248-260.

[8]. Lazard, D., Sastre, X., Frid, M. G., Glukhova, M. A., Thiery, J. P., and Koteliansky, V. E., 1993, Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue. Proc. Nati. Acad. Sci. USA, 90, 999-1003.

[9]. Kellermann, M. G., Sobral, L. M., da Silva, S. D., Zecchin, K. G., Graner, E., Lopes, M. A., Nishimoto, I., Kowalski, L. P., & Coletta, R. D. ,2007. Myofibroblasts in the stroma of oral squamous cell carcinoma are associated with poor prognosis. Histopathology, 51(6), 849–853. https://doi.org/10.1111/j.1365-2559.2007.02873.x

[10]. Amatangelo, M. D., Bassi, D. E., Klein-Szanto, A. J., & Cukierman, E. 2005, Stroma-derived three-dimensional matrices are necessary and sufficient to promote desmoplastic differentiation of normal fibroblasts. The American journal of pathology, 167(2), 475–488. https://doi.org/10.1016/S0002-9440(10)62991-4 

[11]. Desmoulière, A., Guyot, C., & Gabbiani, G. 2004, The stroma reaction myofibroblast: a key player in the control of tumor cell behavior. The International journal of developmental biology, 48(5-6), 509–517. https://doi.org/10.1387/ijdb.041802ad

[12]. Kang, N., Shah, V. H., & Urrutia, R. 2015, Membrane-to-Nucleus Signals and Epigenetic Mechanisms for Myofibroblastic Activation and Desmoplastic Stroma: Potential Therapeutic Targets for Liver Metastasis?. Molecular cancer Research MCR, 13(4), 604–612. https://doi.org/10.1158/1541-7786.MCR-14-0542 

[13]. Catteau, X., Simon, P., & Noël, J. C. 2014, Myofibroblastic reaction is a common event in metastatic disease of breast carcinoma: a descriptive study. Diagnostic pathology, 9, 196. https://doi.org/10.1186/s13000-014-0196-6

[14]. Muir, A. B., Dods, K., Noah, Y., Toltzis, S., Chandramouleeswaran, P. M., Lee, A., Benitez, A., Bedenbaugh, A., Falk, G. W., Wells, R. G., Nakagawa, H., & Wang, M. L. 2015, Esophageal epithelial cells acquire functional characteristics of activated myofibroblasts after undergoing an epithelial to mesenchymal transition. Experimental cell research, 330(1), 102–110. https://doi.org/10.1016/j.yexcr.2014.08.026

[15]. Desmoulière, A., Guyot, C., & Gabbiani, G. 2004, The stroma reaction myofibroblast: a key player in the control of tumor cell behavior. The International journal of developmental biology, 48(5-6), 509–517. https://doi.org/10.1387/ijdb.041802ad

[16]. Blonska, M., Agarwal, N. K., & Vega, F. 2015. Shaping of the tumor microenvironment: Stromal cells and vessels. Seminars in cancer biology, 34, 3–13. https://doi.org/10.1016/j.semcancer.2015.03.002

[17]. Kellermann, M. G., Sobral, L. M., da Silva, S. D., Zecchin, K. G., Graner, E., Lopes, M. A., Nishimoto, I., Kowalski, L. P., & Coletta, R. D. 2007, Myofibroblasts in the stroma of oral squamous cell carcinoma are associated with poor prognosis. Histopathology, 51(6), 849–853. https://doi.org/10.1111/j.1365-2559.2007.02873.x

[18]. van Kempen, L. C., Ruiter, D. J., van Muijen, G. N., & Coussens, L. M. 2003, The tumor microenvironment: a critical determinant of neoplastic evolution. European journal of cell biology, 82(11), 539–548. https://doi.org/10.1078/0171-9335-00346

[19]. Kalluri, R., & Zeisberg, M. 2006, Fibroblasts in cancer. Nature reviews. Cancer, 6(5), 392–401. https://doi.org/10.1038/nrc1877

[20]. Zidar, N., Gale, N., Kambic, V., & Fischinger, J. 2002, Proliferation of myofibroblasts in the stroma of epithelial hyperplastic lesions and squamous carcinoma of the larynx. Oncology, 62(4), 381–385. https://doi.org/10.1159/000065071

[21]. Lynch, C. C., & Matrisian, L. M. 2002, Matrix metalloproteinases in tumor-host cell communication. Differentiation; research in biological diversity, 70(9-10), 561–573. https://doi.org/10.1046/j.1432-0436.2002.700909.x

[22]. Prime, S. S., Davies, M., Pring, M., & Paterson, I. C. 2004. The role of TGF-beta in epithelial malignancy and its relevance to the pathogenesis of oral cancer (part II). Critical reviews in oral biology and medicine: an official publication of the American Association of Oral Biologists, 15(6), 337–347. https://doi.org/10.1177/154411130401500603

[23]. Maeda, G., Chiba, T., Okazaki, M., Satoh, T., Taya, Y., Aoba, T., Kato, K., Kawashiri, S., & Imai, K. 2005, Expression of SIP1 in oral squamous cell carcinomas: implications for E-cadherin expression and tumor progression. International journal of oncology, 27(6), 1535–1541.

[24]. Silzle, T., Randolph, G. J., Kreutz, M., & Kunz-Schughart, L. A. 2004, The fibroblast: sentinel cell and local immune modulator in tumor tissue. International journal of cancer, 108(2), 173–180. https://doi.org/10.1002/ijc.11542

[25]. Olaso, E., Santisteban, A., Bidaurrazaga, J., Gressner, A. M., Rosenbaum, J., & Vidal-Vanaclocha, F. 1997, Tumor-dependent activation of rodent hepatic stellate cells during experimental melanoma metastasis. Hepatology (Baltimore, Md.), 26(3), 634–642. https://doi.org/10.1002/hep.510260315

[26]. David, L., Dulong, V., Coquerel, B., Le Cerf, D., Cazin, L., Lamacz, M., & Vannier, J. P. 2008, Collagens, stromal cell-derived factor-1alpha and basic fibroblast growth factor increase cancer cell invasiveness in a hyaluronan hydrogel. Cell proliferation, 41(2), 348–364. https://doi.org/10.1111/j.1365-2184.2008.00515.x

[27]. Akagawa, S., Ohuchida, K., Torata, N., Hattori, M., Eguchi, D., Fujiwara, K., Kozono, S., Cui, L., Ikenaga, N., Ohtsuka, T., Aishima, S., Mizumoto, K., Oda, Y., & Tanaka, M. 2014, Peritoneal myofibroblasts at metastatic foci promote dissemination of pancreatic cancer. International journal of oncology, 45(1), 113–120. https://doi.org/10.3892/ijo.2014.2391

[28]. Yeung, T. M., Buskens, C., Wang, L. M., Mortensen, N. J., & Bodmer, W. F. (2013). Myofibroblast activation in colorectal cancer lymph node metastases. British journal of cancer, 108(10), 2106–2115. https://doi.org/10.1038/bjc.2013.209

[29] Rukmini, D., Kannan, B., Pandi, C., Pandi, A., Prasad, P., Jayaseelan, V. P., & Arumugam, P., 2024,  Aberrated PSMA1 expression associated with clinicopathological features and prognosis in oral squamous cell carcinoma. Odontology, 112(3), 950–958. https://doi.org/10.1007/s10266-023-00883-0

[30] Kannan, B., Pandi, C., Pandi, A., Jayaseelan, V. P., & Arumugam, P., 2024,  Triggering receptor expressed in myeloid cells 1 (TREM1) as a potential prognostic biomarker and association with immune infiltration in oral squamous cell carcinoma. Archives of oral biology, 161, 105926. https://doi.org/10.1016/j.archoralbio.2024.105926

[31] Ramasubramanian, A., Ramani, P., Kannan, B., & Arumugam, P., 2023, High expression of novel biomarker TBRG4 promotes the progression and invasion of oral squamous cell carcinoma. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 52(8), 738–745. https://doi.org/10.1111/jop.13470

Abstract:

Unicystic ameloblastoma a rare variant of ameloblastoma accounting for 6 % of ameloblastomas is characterized by slow growth and is less aggressive in comparison with ameloblastoma. It is commonly associated with an impacted third molar. However, under rare circumstances it may be associated with impacted mandibular canine.  We present a case of unicystic ameloblastoma in a 31-year-old female patient associated with an impacted mandibular canine. Following treatment, the patient’s sister underwent routine radiographic examination which revealed impacted tooth no 43, a finding exactly similar to her sister's case and a small radiolucency surrounding the crown of tooth no 43 which was surgically extracted to prevent further complications. Both the patients are currently under follow-up.  With the available clinical data, we hypothesize that the occurrence of jaw lesions associated with impacted teeth could be familial. The present cases highlight the importance of routine radiographic examination and follows up and to obtain family history and screen the family members of patients with impacted teeth to prevent potential complications.

References:

[1].   Roos, R. E., Raubenheimer, E. J., van Heerden, W. F., 1994, Clinico-Pathological Study of 30 Unicystic Ameloblastomas. J Dent Assoc S Afr, 49(11), 559–62.

[2].   Zain, R. B., 1986, Unicystic Ameloblastoma: A New Entity? The Medical Journal of Malaysia, 41(4), 370–373.

[3].   Robinson, L., Martinez, M. G., 1977, Unicystig Ameloblastoma. A Prognostically Distinct Entity. Cancer, 40(5), 2278–85.

[4].   Gardner, D. G., Corio, R. L., 1984, Plexiform Unicystic Ameloblastoma: A Variant of Ameloblastoma with a Low‐Recurrence Rate After Enucleation. Cancer, 53(8),1730–5.

[5].   Isacsson. G., Andersson, L., Forsslund, H., Bodin, I., Thomsson, M., 1986, Diagnosis and Treatment of the Unicystic Ameloblastoma. Int J Oral Maxillofac Surg, 15(6),759–64.

[6].   Imase, R., Watanabe, T., 2022, Maxillary Unicystic Ameloblastoma: a Rare Presentation. BMJ Case Reports, 15, e250786.

[7].   Zain, R. B., 1986, Unicystic Ameloblastoma: A New Entity? The Medical journal of Malaysia, 41(4), 370–373.

[8].   Sudhakara, K, Reddy, Girish, S Rao., 2011, Unicystic Ameloblastoma in 6-Year-Old Child and its Significance. World Journal of Dentistry, 2(4), 63-366.

[9].   Reichart, Peter A., and Philpsen, H. P., Odontogenic Tumors and Allied Lesions. Peter a. Reichart, PAPH, Editor. Chicago: Quintessence, 1004, 77–86.

[10].  Heikinheimo, K., Huhtala, J. M., Thiel, A., et al., 2019, The Mutational Profile of Unicystic Ameloblastoma. Journal of Dental Research, 98(1), 54-60. https://doi.org/10.1177/0022034518798810

[11].  Mortazavi, H., Baharvand, M., 2016, Jaw Lesions Associated with Impacted Tooth: A Radiographic Diagnostic Guide. Imaging Sci Dent, 46(3),147–57.

[12].  Al Sineedi F., Aruveetil, Y. A., Kavarodi, A. M., Harbi, S. O., 2018, Bilocular Unicystic Ameloblastoma of The Mandible in a 9-Year-Old Child – A Diagnostic and Management Dilemma. Saudi Dental Journal, 30(3), 250–5.

[13].  Ackermann, G. L., Altini, M., Shearm M.,1988, The Unicystic Ameloblastoma: A Clinicopathological Study of 57 Cases. Journal of Oral Pathology & Medicine, 17(9–10), 541–6.

[14].  Leider, A. S., Eversole, L.R., Barkin, M. E., 1985, Cystic Ameloblastoma. A Clinicopathologic Analysis. Oral Surgery, Oral Medicine, Oral Pathology, 60(6), 624–30.

[15].  Lau, S. L., Samman, N., 2006, Recurrence Related to Treatment Modalities of Unicystic Ameloblastoma: A Systematic Review. Int J Oral Maxillofac Surg, 35(8), 681–90.

[16].  Li, T. J., Browne, R. M., & Matthews, J. B., 1995, Expression of Proliferating Cell Nuclear Antigen (PCNA) and Ki-67 in Unicystic Ameloblastoma. Histopathology, 26(3), 219–228. https://doi.org/10.1111/j.1365-2559.1995.tb01435.x

[17].  Li, Tie-Jun, Wu, Yun-Tang., Yu, Shi-Feng., Yu, Guang-Yan, 2000, Unicystic Ameloblastoma: A Clinicopathologic Study of 33 Chinese Patients. The American Journal of Surgical Pathology, 24(10),1385-1392.

[18].  Li, T., Wu, Y., Yu, S., & Yu, G., 2002, Clinicopathological Features of Unicystic Ameloblastoma With Special Reference to its Recurrence. Zhonghua Kou Qiang Yi Xue Za Zhi Zhonghua Kouqiang Yixue Zazhi. Chinese Journal of Stomatology, 37(3), 210–212.

[19].  Li, T. J., Kitano, M., Arimura, K., & Sugihara, K., 1998, Recurrence of Unicystic Ameloblastoma: A Case Report and Review of the Literature. Archives of Pathology & Laboratory Medicine, 122(4), 371–374.

[20].  Rezin Ahmed, Pradeep, D., Muthusekhar, M. R., Unicystic Ameloblastoma: Case Reports And Review Of Literature2021, Int J Dentistry Oral Sci., 8(7), 3412-3415. doi: http://dx.doi.org/10.19070/2377-8075-21000693

[21].  Vanishree Murugavel, Sunderesh Kamal Chander, & Sonti Sulochana, 2022, Unicystic Plexiform Ameloblastoma of mandible-A rare case entity: Case Report . International Journal of Orofacial Biology, 6(2), 5–9. https://doi.org/10.56501/intjorofacbiol.v6i2.615

[22].  Jitesh, S, Madhulazmi, M, 2021, Management of Unicystic Ameloblastoma – A Review,  International Journal of Scientific Development and Research (IJSDR), 6(3), 517- 520.

[23].  Gültekin, S. E., Aziz, R., Heydt, C., Sengüven, B., Zöller, J., Safi, A. F., 2018, The Landscape of Genetic Alterations in Ameloblastomas Relates to Clinical Features. Virchows Archiv, 472(5), 807–14.

[24].  Geoghegan, S., King, G., Henchliffe, J., Ramsden, S. C., Barry, R. J., Green, A. J., et al.,2018, A Sibling Pair with Cardiofaciocutaneous Syndrome (CFC) Secondary to Braf Mutation with Unaffected Parents—The First Cases of Gonadal Mosaicism in CFC? Am J Med Genet, 176(7), 1637–40.

[25].  Łangowska-Adamczyk, H., Karmańska, B., 2001, Similar Locations of Impacted and Supernumerary Teeth in Monozygotic Twins: A Report of 2 Cases. American Journal of Orthodontics and Dentofacial Orthopedics,119(1), 67–70.

Abstract:

Endometriosis refers to the existence of functional endometrial glands and tissue in locations beyond the uterus. This condition frequently leads to persistent pelvic discomfort, occasionally severe, and inflammation is recognized as a contributing factor in the development of endometriosis. Finding a non-invasive indicator for endometriosis would greatly assist in its early detection and treatment. Therefore, the present study was conducted to study the levels of inflammatory mediators - Complement 3 (C3) and C-reactive protein (CRP) in endometriosis condition. The study was conducted in 40 women who have been laparoscopically diagnosed with endometriosis, and the levels of C3 and serum CRP were estimated in the plasma by ELISA analysis. The results demonstrated significant rise in the levels of serum CRP and C3 in endometriotic women as compared to the levels in non-endometriotic women. Due to the altered regulation of the complement system and the effects of pelvic adhesions and deep infiltrating endometriosis, there is a significant rise in the levels of complement 3 and serum CRP respectively. While immune infiltration and constant inflammatory milieu foster the disease, the biggest pathological consequences arise from aberrant complement activation. Hence, with the aid of pre-clinical and clinical trials, a detailed strategy can be formulated to suppress complement activation and alleviate the devastating effects of endometriosis.

References:

[1]. Giudice, L.C., 2004. kao LC. Endometriosis. Lancet, 364(9447), pp.1789-99.

[2]. Thubert, T., Santulli, P., Marcellin, L., Menard, S., M'Baye, M., Streuli, I., Borghese, B., de Ziegler, D. and Chapron, C., 2014. Measurement of hs-CRP is irrelevant to diagnose and stage endometriosis: prospective study of 834 patients. American Journal of Obstetrics and Gynecology, 210(6), pp.533-e1.

[3]. Abrão, M.S., Podgaec, S., Filho, B.M., Ramos, L.O., Pinotti, J.A. and de Oliveira, R.M., 1997. The use of biochemical markers in the diagnosis of pelvic endometriosis. Human Reproduction, 12(11), pp.2523-2527.

[4]. Wu, M.H., Hsiao, K.Y. and Tsai, S.J., 2015. Endometriosis and possible inflammation markers. Gynecology and minimally invasive therapy, 4(3), pp.61-67.

[5]. Mu, F., Harris, H.R., Rich-Edwards, J.W., Hankinson, S.E., Rimm, E.B., Spiegelman, D. and Missmer, S.A., 2018. A prospective study of inflammatory markers and risk of endometriosis. American journal of epidemiology, 187(3), pp.515-522.

[6]. Kokot, I., Piwowar, A., Jędryka, M., Sołkiewicz, K. and Kratz, E.M., 2021. Diagnostic significance of selected serum inflammatory markers in women with advanced endometriosis. International journal of molecular sciences, 22(5), p.2295.

[7]. Pepys, M.B. and Hirschfield, G.M., 2003. C-reactive protein: a critical update. The Journal of clinical investigation, 111(12), pp.1805-1812.

[8]. Abrão, M.S., Podgaec, S., Filho, B.M., Ramos, L.O., Pinotti, J.A. and de Oliveira, R.M., 1997. The use of biochemical markers in the diagnosis of pelvic endometriosis. Human Reproduction, 12(11), pp.2523-2527.

[9]. Matarese, G., Alviggi, C., Sanna, V., Howard, J.K., Lord, G.M., Carravetta, C., Fontana, S., Lechler, R.I., Bloom, S.R. and De Placido, G., 2000. Increased leptin levels in serum and peritoneal fluid of patients with pelvic endometriosis. The Journal of Clinical Endocrinology & Metabolism, 85(7), pp.2483-2487.

[10]. Xavier, P., Belo, L., Beires, J., Rebelo, I., Martinez-de-Oliveira, J., Lunet, N. and Barros, H., 2006. Serum levels of VEGF and TNF-α and their association with C-reactive protein in patients with endometriosis. Archives of gynecology and obstetrics, 273, pp.227-231.

[11]. Kianpour, M., Nematbakhsh, M. and Ahmadi, S.M., 2012. C-reactive protein of serum and peritoneal fluid in endometriosis. Iranian journal of nursing and midwifery research, 17(2 Suppl1), pp.S115-S119.

[12]. Bahari, H., Zeraattalab-Motlagh, S., Sajadi Hezaveh, Z., Namkhah, Z., Golafrouz, H., Taheri, S. and Sahebkar, A., 2024. The Effects of Sumac Consumption on Inflammatory and Oxidative Stress Factors: A Systematic Review of Randomized Clinical Trials. Current Pharmaceutical Design, 30(27), pp.2142-2151.

[13]. Rekker, K., Saare, M., Eriste, E., Tasa, T., Kukuškina, V., Roost, A.M., Anderson, K., Samuel, K., Karro, H., Salumets, A. and Peters, M., 2017. High-throughput mRNA sequencing of stromal cells from endometriomas and endometrium. Reproduction, 154(1), pp.93-100.

[14]. Suryawanshi, S., Huang, X., Elishaev, E., Budiu, R.A., Zhang, L., Kim, S., Donnellan, N., Mantia-Smaldone, G., Ma, T., Tseng, G. and Lee, T., 2014. Complement pathway is frequently altered in endometriosis and endometriosis-associated ovarian cancer. Clinical Cancer Research, 20(23), pp.6163-6174.

[15]. Ahn, S.H., Khalaj, K., Young, S.L., Lessey, B.A., Koti, M. and Tayade, C., 2016. Immune-inflammation gene signatures in endometriosis patients. Fertility and sterility, 106(6), pp.1420-1431.

[16]. Babah, O.A., Ojewunmi, O.O., Onwuamah, C.K., Udenze, I.C., Osuntoki, A.A. and Afolabi, B.B., 2023. Serum concentrations of IL-16 and its genetic polymorphism rs4778889 affect the susceptibility and severity of endometriosis in Nigerian women. BMC Women's Health, 23(1), p.253.

[17]. Bakhashab, S., Banafea, G.H., Ahmed, F., Alsolami, R., Schulten, H.J., Gauthaman, K., Naseer, M.I. and Pushparaj, P.N., 2024. Acute and prolonged effects of interleukin-33 on cytokines in human cord blood-derived mast cells. Immunology Letters, 269, p.106908. 

[18]. Tehrani, S.D., Hosseini, A., Shahzamani, M., Heidari, Z., Askari, G., Majeed, M., Sahebkar, A. and Bagherniya, M., 2024. Evaluation of the effectiveness of curcumin and piperine co-supplementation on inflammatory factors, cardiac biomarkers, atrial fibrillation, and clinical outcomes after coronary artery bypass graft surgery. Clinical Nutrition ESPEN, 62, pp.57-65.

[19]. Vodolazkaia, A., Bossuyt, X., Fassbender, A., Kyama, C.M., Meuleman, C., Peeraer, K., Tomassetti, C. and D'Hooghe, T.M., 2011. A high sensitivity assay is more accurate than a classical assay for the measurement of plasma CRP levels in endometriosis. Reproductive Biology and Endocrinology, 9, pp.1-9.

[20]. Ruiz, L.A., Dutil, J., Ruiz, A., Fourquet, J., Abac, S., Laboy, J. and Flores, I., 2011. Single-nucleotide polymorphisms in the lysyl oxidase-like protein 4 and complement component 3 genes are associated with increased risk for endometriosis and endometriosis-associated infertility. Fertility and sterility, 96(2), pp.512-515.

Abstract:

Premenstrual syndrome refers to distressing physical, psychological, and behavioural symptoms that are not caused by organic disease it regularly occurs before the menstrual cycle and subsides after the onset of menses. In developing countries like India, the stigma surrounding menstruation is a big barrier to seeking help for physical and mental discomfort. As healthcare workers live a more hectic life more knowledge and awareness to be given. Hence, this study was conducted to estimate the prevalence of premenstrual syndrome among healthcare workers. A survey was taken with a questionnaire among 390 healthcare workers 18-35 years of age containing signs and symptoms of premenstrual syndrome. Data collected were analysed through tables using proportions and percentages. In this current study, PMS was present among 52.3% of the healthcare workers while 47.7% had no PMS. The mean age of menarche among healthcare workers with PMS was 13. ±3years. The association between physical activity, BMI, Sleep habits, diet, and PMS was found to be highly significant. This study shows the prevalence of PMS among women in healthcare setups. Therefore measures to be taken to reduce the incidence, to conduct awareness programs, improving knowledge about PMS thereby improving quality of life.

References:

[1]. Shruti, V., Archana Nimbalkar, Ajay, G., Phatak, Somashekhar, M., Nimbalkar, 2019, Premenstural Syndrome in Anand District, Gujarat: A Cross-Sectional Survey, Journal of Family Medicine and Primary Care, 8(2):640, IP:85.218.198.23, https://www.researchgate.net/publication/331381816_Premenstrual_syndrome_in_Anand_District_Gujarat_A_cross-sectional_surve

[2]. Bhuvaneswari, K., Rabindran, P., Bharadwaj, B., 2019, Prevalence of Premenstrual Syndrome and its Impact on Quality of Life Among Selected College Students in Puducherry. The National Medical Journal of India, 32:17-19, https://nmji.in/prevalence-of-premenstrual-syndrome-and-its-impact-on-quality-of-life-among-selected-college-students-in-puducherry/

[3]. Priyanka Malhotra, Suresh Kumar Sharma, Ravinder Kaur, Urvashi, Vanshika, Rakhi Gaur and Shiv Mudgal, 2020, Premenstrual Syndrome and Health Related Quality of Life Among Young Adult Females at Northern India: A Cross-Sectional Study. Clin Psychiatry Journal, Vol.6 No.1:65 https://www.researchgate.net/publication/344768200_Pre_menstrual_syndrome_and_health_related_quality_of_life_among_young_adult_females_at_Northern_India_A_cross-sectional_study 

[4]. Madhu Gupta, Devakshi Dua, Harsheen Kaur, and Sandeep Grover, 2019, Prevalence of Premenstrual Dysphoric Disorder Among School-Going Adolescent Girls. Industrial Psychiatry Journal, Jul 1;28(2):198-202. https://pubmed.ncbi.nlm.nih.gov/33223711/

[5]. Sonal Kulshrestha, Anisa, M., Durrani, 2019., Prevalence of Menstrual Disorders and their Association with Physical Activety in Adolescent Girls of Aligarh City. International Journal of Health Sciences and Research, Vol.9; Issue:8; 384-93. https://www.ijhsr.org/IJHSR_Vol.9_Issue.8_Aug2019/53.pdf

[6]. Iman Mohmad Wah by Salem Mashael Yahya Alsamti, Manal Abdulaziz Murad, 2020, Predictors of Premenstrual Syndrome Among Female Students at Governmental Secondary Schools in Jeddah, Saudi Arabia: A cross-sectional study. The Egyptian Journal of Hospital Medicine, Vol.78(2):337-47. https://ejhm.journals.ekb.eg/article_76614_b50693256fe1bf6cdb1ad8cf3da9752a.pdf

[7]. Indira Shresthaa, Rita Giri, 2021, Knowledge and Prevalence Regarding Premenstrual Syndrome Among Adolescents in Morang District, Nepal. Current Women's Health Reviews. vol 1;16(3):214-9. https://www.researchgate.net/publication/340833022_Knowledge_and_Prevalence_Regarding_Premenstrual_Syndrome_among_Adolescents_in_Morang_District_Nepal

[8]. Riya, S., Shah and Donald, S., Christian, 2020, Association of Socio-Demographic, Dietary and Lifestyle Factors with Premenstrual Syndrome (PMS) Among Undergraduate Medical Students of A Tertiary Care Institute in Ahmedabad, Gujarat. Journal of Family Medicine and Primary Care, Vol 1;9(11):5719-24. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842494/

[9]. Abhijit Dutta, Avinash Sharma, 2021, Prevalence of Premenstrual Syndrome and Premenstrual Dysphonic Disorder in India: A Systematic Review and Meta-Analysis, Health Promotion Perspectives. Vol 11(2):161. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233671/

[10]. Riddhi Sahu , Suresh Lal Barnwal, Santosh Vishvakarma, 2022, Prevalence and Severity Of Premenstrual Syndrome Among Young Females in Chhattisgarh, India. Asian Pacific Journal of Health Sciences, Vol 9(1):120-5, https://www.researchgate.net/publication/358200051_Prevalence_and_Severity_of_Premenstrual_Syndrome_among_Young_Females_in_Chhattisgarh_India

[11]. Siham Lghoul, Mohamed Loukid, Abdelhafid Benksim and Mohamed Kamal Hilali, 2022, Factors Associated with Premenstrual Syndrome: A Cross-Sectional Study of Female Adolescents (Morocco), European Journal of Medical and Health Sciences, Vol4(6):1-5, https://www.researchgate.net/publication/365282050_Factors_Associated_with_Premenstrual_Syndrome_A_Cross-Sectional_Study_of_Female_Adolescents_Morocco

[12]. Himalini Nandakumar, MaheshKumar Kuppusamy, Lavanya Sekhar, Padmavathi Ramaswamy, 2023, Prevalence of Premenstrual Syndrome Among Students–Stress a Potential Risk Factor. Clinical Epidemiology and Global Health, Vol23:101368, https://www.sciencedirect.com/unsupported_browser

[13]. Farzaneh Babapour, Forouzan Elyasi, Zohreh Shahhosseini, Monirolsadate Hosseini Tabaghdehi, 2023, The Prevalence of Moderate–Severe Premenstrual Syndrome and Premenstrual Dysphoric Disorder and the Related Factors in High School Students, A Cross‐Sectionalstudy, Neuropsychopharmacol Reports, Vol 43(2):249-54, https://pubmed.ncbi.nlm.nih.gov/37154790/

[14]. Michelle Proctor, CynthiaFarquhar, 2006, Premenstrual Syndrome. BMJ Clinical Evidence, Vol 332(7550): 1134–1138, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1459624/

[15]. Kimberly Ann Yonkers, Shaughn O’Brien ,P M., Elias Eriksson, 2008, Premenstrual syndrome. The Lancet, Vol 371(9619), 1200-1210. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118460/

[16]. Abhijit Dutta , Avinash Sharma , 2021, Prevalence of Premenstrual Syndrome And Premenstrual Dysphoric Disorder in India: A Systematic Review and Meta-Analysis, Health Promot Perspect, 11(2):161-170, https://pubmed.ncbi.nlm.nih.gov/34195039/

[17]. Mohan, M. P, Mohan, P., 2017, Prevalence and Severity of Premenstrual Syndrome in Medical Students of a Tertiary Care Hospital in India, Journal of Clinical and Diagnostic Research, Vol11(7),OC25-OC28. doi:10.7860/JCDR/2017/25768.10246.

[18]. Shana Parveen Janu, M., Kanthi, Renjitha Bhaskaran, 2024, Premenstrual Syndrome, Coping Mechanisms and Associated Factors Among Female Students of a Health Sciences Campus in South India, International Journal of Reproduction, Vol. 13 No. 7, https://www.ijrcog.org/index.php/ijrcog/article/view/14188

[19]. Bhuvaneswari, K., Porkodi Rabindran, Balaji Bharadwaj, 2019, Prevalence of Premenstrual Syndrome and its Impact on Quality of Life Among Selected College Students in Puducherry, Natl Med J India, Vol 32:17-19, https://nmji.in/prevalence-of-premenstrual-syndrome-and-its-impact-on-quality-of-life-among-selected-college-students-in-puducherry/

[20]. Fatimah Alkhamis, Layla Abd Alaziz Almzraq, Zahraa Khaled Alshayeb, Zainab Yaseen AL-Jaziri, 2021, Prevalence of Premenstrual Syndrome Among Medical Students in King Faisal University in Alahssa-Saudi Arabia, Medical Science,Vol 25(114), https://www.researchgate.net/publication/366596221_Prevalence_of_premenstrual_syndrome_among_medical_students_in_King_Faisal_University_in_Alahssa-Saudi_Arabia

Abstract:

Road traffic accidents are one of the world's major sources of death and injury, with low- and middle-income nations suffering the most from them, despite having 60% of the world's automobiles. Two-wheeler accidents lead to a high rate of fatalities and severe injuries, disproportionately affecting young, economically active individuals and resulting in a considerable loss of productive life years. The impact of this death toll on society's socioeconomic output is valuable. This study evaluated the causes and patterns of injuries associated with two-wheeler road traffic accidents in the Chengalpattu region. In a Tertiary Care Hospital in Chengalpattu district, 296 two-wheeler RTA victims participated in six-month cross-sectional research from January to June 2023. Interviews with accident victims were conducted using a pre-tested, semi-structured questionnaire intended for injury patterns in road traffic accidents. Data were analysed using SPSS version 22. Among the participants in the study, 17.1% of the cases were linked to traffic accidents; the majority 51.7% occurred in the under-32 age group, and 80.7% of the cases involved males. Head and neck injuries accounted for 54.4% of all injuries, with upper extremities coming in second with 28.4%. During a collision, alcohol was consumed by 25.7% of the drivers who were involved. The majority of accidents, 37.8%, occur during the night hours from 6 p.m. to 12 midnight. The data reveals a concerning trend of head and limb injuries predominating, highlighting the vulnerability of motorcyclists and scooter riders to severe trauma, particularly to the head and extremities.

References:

[1]. Khalaf, M., Rosen, H., Mitra, S., Neki, K., Mbugua L, Hyder A, Paichadze N Estimating the Burden of Disability From Road Traffic Injuries in 5 Low- and Middle-Income Countries: Protocol for a Prospective Observational Study JMIR Res Protoc 2023;12:e40985 https://www.researchprotocols.org/2023/1/e40985 

[2]. Road traffic injuries. (n.d.). Retrieved April 25, 2024, from https://www.who.int/news room/fact-sheets/detail/road-traffic-injuries

[3]. Transportation Safety | Transportation Safety | Injury Center | CDC. (2023, July 24). https://www.cdc.gov/transportationsafety/index.html

[4]. Ministry of Road Transport and Highways releases Annual Report on ‘Road Accidents in India-2022”. (n.d.). Retrieved July 23, 2024, from https://www.pib.gov.in/www.pib.gov.in/Pressreleaseshare.aspx?PRID=1973295

[5]. SDG Target 3.6 | Road traffic injuries: By 2030, halve the number of global deaths and injuries from road traffic accidents. (n.d.). Retrieved April 30, 2024, from https://www.who.int/data/gho/data/themes/topics/indicator-groups/indicator-groupdetails/GHO/SDG-target-3.6-road-traffic-injuries

[6]. Global status report on road safety 2018. (n.d.). Retrieved April 30, 2024, from https://www.who.int/publications-detail-redirect/9789241565684

[7]. Pathak, A., Dev, R., & Awasthi, P., 2020, Study of Injuries among Road Traffic Accidents Victim at LLR Hospital, GSVM Medical College, Kanpur U.P.

[8]. Kakkar, R., Aggarwal, P., Kakkar, M., Deshpande, K., & Gupta, D, 2014, Road traffic accident: a retrospective study. Indian Journal of Scientific Research.

[9]. Solanki, L., & Mittal, H., 2016, an epidemiological study of road traffic accident cases at a tertiary care hospital in Udaipur. International Journal of Current Research and review.

[10]. Adavikottu, A., & Velaga, N. R., 2023, Analysis of speed reductions and crash risk of aggressive drivers during emergent pre-crash scenarios at unsignalized intersections. Accident Analysis & Prevention, 187, 107088. https://doi.org/10.1016/j.aap.2023.107088

[11]. Balogun, J. A., & Abereoje, O. K., 1992, Pattern of Road Traffic Accident Cases in a Nigerian university teaching hospital between 1987 and 1990. The Journal of Tropical Medicine and Hygiene, 95(1), 23–29.

[12]. Qirjako, G., Burazeri, G., Hysa, B., & Roshi, E., 2008, Factors associated with fatal traffic accidents in Tirana, Albania: Cross-sectional study. Croatian Medical Journal, 49(6), 734–740. https://doi.org/10.3325/cmj.2008.49.734

[13]. Gicquel, L., Ordonneau, P., Blot, E., Toillon, C., Ingrand, P., & Romo, L., 2017, Description of Various Factors Contributing to Traffic Accidents in Youth and Measures Proposed to Alleviate Recurrence. Frontiers in Psychiatry, 8. https://doi.org/10.3389/fpsyt.2017.00094

[14]. Ivascu, L., & Cioca, L.-I., 2019, Occupational Accidents Assessment by Field of Activity and Investigation Model for Prevention and Control. Safety, 5(1), Article 1. https://doi.org/10.3390/safety5010012

[15]. Misra, P., Majumdar, A., Misra, M. C., Kant, S., Gupta, S. K., Gupta, A., & Kumar, S., 2017, Epidemiological study of patients of road traffic injuries attending the emergency department of a trauma center in New Delhi. Indian Journal of Critical Care Medicine: Peer-Reviewed, Official Publication of Indian Society of Critical Care Medicine, 21(10), 678.

[16]. Neeluri, R., & Anga, V. S., 2018, A study on victims of road traffic accidents attending casualty in a tertiary care hospital, Khammam. International Journal of Community Medicine and Public Health, 5(7), 3034. https://doi.org/10.18203/2394-6040.ijcmph20182644

[17]. Zhao, X., Zhang, X., & Rong, J., 2014, Study of the Effects of Alcohol on Drivers and Driving Performance on Straight Road. Mathematical Problems in Engineering, 2014, e607652. https://doi.org/10.1155/2014/607652

[18]. Patan, S., Narapureddy, B. R., C., C., Hussain, R., & Basha, K., 2018, A study on injuries of road traffic accident victims attending a tertiary care hospital, Tirupathi. International Journal Of Community Medicine And Public Health, 5, 2357. https://doi.org/10.18203/23946040.ijcmph20182158

[19]. Al-Thaifani, A. A., Al-Rabeei, N. A., & Dallak, A. M., 2016, Study of the Injured Persons and the Injury Pattern in Road Traffic Accident in Sana’a City, Yemen. Advances in Public Health, 2016, 1–5. https://doi.org/10.1155/2016/4138163

[20]. Shrivastava, S. R., Pandian, P., & Shrivastava, P. S., 2014, Pre-hospital care among victims of road traffic accident in a rural area of Tamil Nadu: A cross-sectional descriptive study. Journal of Neurosciences in Rural Practice, 5(Suppl 1), S33–S38. https://doi.org/10.4103/0976-3147.145198

[21]. Jha, N., Srinivasa, D., Roy, G., Jagdish, S., & Minocha, R., 2004, Epidemiological Study of Road Traffic Accident Cases: A Study from South India. Indian Journal of Community Medicine, 29(1).

[22]. Trivedi, A., & Rawal, D., 2011, Prevalence of road traffic accidents and driving practices among young drivers. Healthline, Journal of Indian Association of Preventive and Social Medicine.

[23]. Morais Neto, O. L. de, Malta, D. C., Mascarenhas, M. D. M., Duarte, E. C., Silva, M. M. A. da, Oliveira, K. B. de, Lima, C. M., & Porto, D. L., 2010, [Risk factors for road traffic injury among adolescents in Brazil: National Adolescent School-based Health Survey (PeNSE)]. Ciencia & Saude Coletiva, 15 Suppl 2, 3043–3052. https://doi.org/10.1590/s1413-81232010000800009

[24]. Gururaj, G., 2002, Epidemiology of traumatic brain injuries: Indian scenario. Neurological Research, 24(1), 24–28. https://doi.org/10.1179/016164102101199503.

[25]. Ganveer, G. B., & Tiwari, R. R., 2005, Injury pattern among non-fatal road traffic accident cases: A cross-sectional study in Central India. Indian Journal of Medical Sciences, 59(1), 9–12.

[26]. Ranjan, Dr. R., kumar, Dr. D., & Lal, D., 2017, Pattern And Distribution of Injuries in Fatal Road Traffic Accident Cases. IOSR Journal of Dental and Medical Sciences, 16, 71–74. https://doi.org/10.9790/0853-1603027174.

[27]. Shah, A., & Jarwani, B., 2014, Research article Study of patients of road traffic accidents arriving in the emergency department (ED) of V.S. hospital at Ahmedabad city, single centre pilot study. https://www.semanticscholar.org/paper/Research-article-Study-of-patients-of-road-traffic-Shah-Jarwani/011a9b6891c361d26638c48c998cf204516c1be1.

[28]. Shrestha, V. L., Bhatta, D. N., Shrestha, K. M., Gc, K. B., & Paudel, S., 2017, Factors and Pattern of Injuries Associated with Road Traffic Accidents in Hilly District of Nepal. Journal of Biosciences and Medicines, 05(12), 88–100. https://doi.org/10.4236/jbm.2017.512010.

[29]. Nasiri, N., Nazari, P., Kamali, A., Sharifi, A., & Sharifi, H., 2019, Factors contributing to fatal road traffic accidents in the South of Kerman during the period from 2013 to 2017, Iran. Journal of Occupational Health and Epidemiology, 8, 6–11.https://doi.org/10.29252/johe.8.1.6.

[30]. Rolison, J. J., Regev, S., Moutari, S., & Feeney, A., 2018, What are the factors that contribute to road accidents? An assessment of law enforcement views, ordinary drivers’ opinions, and road accident records. Accident Analysis & Prevention, 115, 11–24. https://doi.org/10.1016/j.aap.2018.02.025.

Abstract:

Acute gouty arthritis (AGA) is marked by a swift inflammatory response triggered by the accumulation of monosodium urate (MSU) crystals around the joints, often linked to hyperuricemia (HUA). Traditional approaches in AGA management usually prove inadequate, prompting a recent focus on the potential of herbal medicines (HMs) for AGA treatment. This review aims to outline the pharmacological mechanisms of HMs in treating AGA, exploring their active components, extracts, and prescriptions, and discussing relevant molecular targets extensively. Scientific publications on anti-AGA HMs were gathered from diverse journals and databases like PubMed, Elsevier, and Google Scholar. The review identified numerous therapeutic targets for AGA treated by HMs through in vitro and in vivo studies. HMs and their active ingredients were found to alleviate AGA symptoms by influencing various immune cell targets. The study revealed that HMs have multiple therapeutic targets that effectively address AGA symptoms through in vitro and in vivo studies. The review systematically categorized the anti-AGA properties of HMs, highlighting phenolic, flavonoid, terpenoid, and alkaloid compounds as key ingredients for AGA improvement. HMs and their active ingredients are shown to enhance efficacy by interacting with multiple targets, with NLRP3 being a primary therapeutic focus. Further research is needed to fully understand how HMs alleviate AGA due to the complex nature of HMs.

References:

[1].   Tausche, A.-K., Jansen, T. L., Schröder, H.-E., Bornstein, SR, Aringer, M, Müller-Ladner, U. 2009. Gout—current diagnosis and treatment. Deutsches Ärzteblatt International 106:549.

[2].   Choi, H. K., McCormick, N., Yokose, C., 2022. Excess comorbidities in gout: the causal paradigm and pleiotropic approaches to care. Nature Reviews Rheumatology 18:97-111.

[3].   Liu, W., Peng, J., Wu, Y., Ye, Z., Zong, Z., et al. 2023. Immune and inflammatory mechanisms and therapeutic targets of gout: An update. International immunopharmacology 121:110466.

[4].   Zhao, J., Wei, K., Jiang, P., Chang, C., Xu, L., et al. 2022. Inflammatory response to regulated cell death in gout and its functional implications. Frontiers in immunology 13:888306.

[5].   Zhao, L., Ye, W., Zhu, Y., Chen, F., Wang, Q., et al. 2022. Distinct macrophage polarization in acute and chronic gout. Laboratory Investigation 102:1054-63

[6].   Keller, S. F., Mandell, B. F., 2021. Management and cure of gouty arthritis. Medical Clinics 105:297-310.

[7].   Wang, S., Liu, W., Wei, B., Wang, A., Wang, Y., et al. 2024. Traditional Herbal Medicine: Therapeutic Potential in Acute Gouty Arthritis. Journal of Ethnopharmacology:118182.

[8].   Tai, F. W. D., McAlindon, M. E., 2021. Non-steroidal anti-inflammatory drugs and the gastrointestinal tract. Clinical Medicine 21:131-4.

[9].   Sun, X., Yang, L., Sun, H., Sun, Y., Wei, S., et al. 2023. TCM and related active compounds in the treatment of gout: the regulation of signaling pathway and urate transporter. Frontiers in Pharmacology 14:1275974.

[10].  Liu, L., Zhu, L., Liu, M., Zhao, L., Yu, Y., et al. 2022. Recent insights into the role of macrophages in acute gout. Frontiers in immunology 13:955806.

[11].  Liu‐Bryan, R., Scott, P., Sydlaske, A., Rose, DM, Terkeltaub, R. 2005. Innate immunity conferred by Toll‐like receptors 2 and 4 and myeloid differentiation factor 88 expression is pivotal to monosodium urate monohydrate crystal–induced inflammation. Arthritis & Rheumatism 52:2936-46.

[12].  Cobo, I., Murillo-Saich, J, Alishala, M, Guma, M. 2023. Epigenetic and Metabolic Regulation of Macrophages during Gout. Gout, Urate, and Crystal Deposition Disease 1:137-51.

[13].  Liu, C., Zhou, M., Jiang, W., Ye., S., Tian, S., et al. 2022. GPR105-targeted therapy promotes gout resolution as a switch between NETosis and apoptosis of neutrophils. Frontiers in Immunology 13:870183.

[14].  Li, C., Wu, C., Li, F., Xu, W., Zhang, X., et al. 2024. Targeting Neutrophil Extracellular Traps in Gouty Arthritis: Insights into Pathogenesis and Therapeutic Potential. Journal of Inflammation Research:1735-63.

[15].  Liu, L., Shan, L., Wang, H., Schauer, C., Schoen, J., et al. 2023. Neutrophil Extracellular Trap–Borne Elastase Prevents Inflammatory Relapse in Intercritical Gout. Arthritis & Rheumatology 75:1039-47.

[16].  Di, H., Han, X., Yin, Y., Zhang, Y., Zeng, X., 2024. Role of Chemotaxis of Vδ2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis. Journal of Inflammation Research:721-36.

[17].  Wang, B., Chen, S., Qian, H., Zheng, Q., Chen, R., et al. 2020. Role of T cells in the pathogenesis and treatment of gout. International immunopharmacology 88:106877.

[18].  Zhao, J., Liu, H., Hong, Z., Luo, W., Mu, W., et al. 2023. Tanshinone I specifically suppresses NLRP3 inflammasome activation by disrupting the association of NLRP3 and ASC. Molecular Medicine 29:84.

[19].  Campbell, K. S., Hasegawa, J., 2013. Natural killer cell biology: an update and future directions. Journal of Allergy and Clinical Immunology 132:536-44.

[20].  O’Brien, K. L., Finlay, D. K., 2019. Immunometabolism and natural killer cell responses. Nature Reviews Immunology 19:282-90.

[21].  Li, D., Yuan, S., Deng, Y., Wang, X., Wu, S., et al. 2023. The dysregulation of immune cells induced by uric acid: mechanisms of inflammation associated with hyperuricemia and its complications. Frontiers in Immunology 14:1282890.

[22].  Wei, L., Yuanhao, W., Bin, X., Yue, J., Shumin, Z., et al. 2021. Effect of integrated Traditional Chinese and Western Medicine on gout. Journal of Traditional Chinese Medicine 41.

[23].  Zhou, Q., Sun, H.-j., Liu, S.-m., 2023. Effects of total saponins from Dioscorea nipponica makino on monosodium urate-induced M1-polarized macrophages through arachidonic acid signaling pathway: an in vitro study. Chinese journal of integrative medicine 29:44-51.

[24].  Meng, Q., Meng, W., Bian, H., Zheng, F., Gu, H., et al. 2021. Total glucosides of paeony protects THP-1 macrophages against monosodium urate-induced inflammation via MALAT1/miR-876-5p/NLRP3 signaling cascade in gouty arthritis. Biomedicine & Pharmacotherapy 138:111413.

[25].  Lin, X., Shao, T., Huang, L., Wen, X., Wang, M., et al. 2020. Simiao decoction alleviates gouty arthritis by modulating proinflammatory cytokines and the gut ecosystem. Frontiers in Pharmacology 11:955.

[26].  Bai, J., Zhang, Y., Tang, C., Hou, Y., Ai, X., et al. 2021. Gallic acid: Pharmacological activities and molecular mechanisms involved in inflammation-related diseases. Biomedicine & pharmacotherapy 133:110985.

[27].  Ren, G., Sun, A., Deng, C., Zhang, J., Wu, X., et al. 2015. The anti-inflammatory effect and potential mechanism of cardamonin in DSS-induced colitis. American Journal of Physiology-Gastrointestinal and Liver Physiology 309:G517-G27.

[28].  Wang, X., Chi, J., Dong, B., Xu, L., Zhou, Y., et al. 2021. MiR‐223‐3p and miR‐22‐3p inhibit monosodium urate‐induced gouty inflammation by targeting NLRP3. International journal of rheumatic diseases 24:599-607.

[29].  Gong, Z., Wang, Y., Li, L., Li, X., Qiu, B., Hu, Y., 2023. Cardamonin alleviates chondrocytes inflammation and cartilage degradation of osteoarthritis by inhibiting ferroptosis via p53 pathway. Food and Chemical Toxicology 174:113644.

[30].  Lin, X., Wang, H., An, X., Zhang, J., Kuang, J., et al. 2021. Baeckein E suppressed NLRP3 inflammasome activation through inhibiting both the priming and assembly procedure: Implications for gout therapy. Phytomedicine 84:153521.

[31].  Wang, L., Zhu, L., Duan, C., Li, L., Chen, G., 2020. Total saponin of Dioscorea collettii attenuates MSU crystal‑induced inflammation via inhibiting the activation of the NALP3 inflammasome and caspase‑1 in THP‑1 macrophages. Molecular medicine reports 21:2466-74.

[32].  Dinesh, P., Rasool, M., 2017. Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats. International immunopharmacology 44:26-37.

[33].  Xu, L., Liu, X., Zhang, Y., Jia, T., Li, L., et al. 2022. Tanshinone IIA improves acute gouty arthritis in rats through regulating neutrophil activation and the NLRP3 inflammasome. Disease markers 2022:5851412.

[34].  He, M., Hu, C., Chen, M., Gao, Q., Li, L., Tian W., 2022. Effects of Gentiopicroside on activation of NLRP3 inflammasome in acute gouty arthritis mice induced by MSU. Journal of natural medicines:1-10

[34]. Cao, Y., 2021. Icariin alleviates MSU‐induced rat GA models through NF‐κB/NALP3 pathway. Cell biochemistry and function 39:357-66.

Abstract:

Road traffic accidents are one of the world's major sources of death and injury, with low- and middle-income nations suffering the most from them, despite having 60% of the world's automobiles. Two-wheeler accidents lead to a high rate of fatalities and severe injuries, disproportionately affecting young, economically active individuals and resulting in a considerable loss of productive life years. The impact of this death toll on society's socioeconomic output is valuable. This study evaluated the causes and patterns of injuries associated with two-wheeler road traffic accidents in the Chengalpattu region. In a Tertiary Care Hospital in Chengalpattu district, 296 two-wheeler RTA victims participated in six-month cross-sectional research from January to June 2023. Interviews with accident victims were conducted using a pre-tested, semi-structured questionnaire intended for injury patterns in road traffic accidents. Data were analysed using SPSS version 22. Among the participants in the study, 17.1% of the cases were linked to traffic accidents; the majority 51.7% occurred in the under-32 age group, and 80.7% of the cases involved males. Head and neck injuries accounted for 54.4% of all injuries, with upper extremities coming in second with 28.4%. During a collision, alcohol was consumed by 25.7% of the drivers who were involved. The majority of accidents, 37.8%, occur during the night hours from 6 p.m. to 12 midnight. The data reveals a concerning trend of head and limb injuries predominating, highlighting the vulnerability of motorcyclists and scooter riders to severe trauma, particularly to the head and extremities.

References:

[1].   Wada, N., Sakamoto, T., & Matsugo, S. (2015). Mycosporine-like amino acids and their derivatives as natural antioxidants. Antioxidants, 4(3), 603-646.

[2].   Kageyama, H., &Waditee-Sirisattha, R. (2018). Mycosporine-like amino acids as multifunctional secondary metabolites in cyanobacteria: From biochemical to application aspects. Studies in natural products chemistry, 59, 153-194.

[3].   Rosic, N. N. (2019). Mycosporine-like amino acids: making the foundation for organic personalised sunscreens. Marine drugs, 17(11), 638.

[4].   Chrapusta, E., Kaminski, A., Duchnik, K., Bober, B., Adamski, M., &Bialczyk, J. (2017). Mycosporine-like amino acids: Potential health and beauty ingredients. Marine drugs, 15(10), 326.

[5].   Geraldes, V., & Pinto, E. (2021a). Mycosporine-like amino acids (MAAs): Biology, chemistry and identification features. Pharmaceuticals, 14(1), 63.

[6].   Raj, S., Kuniyil, A. M., Sreenikethanam, A., Gugulothu, P., Jeyakumar, R. B., & Bajhaiya, A. K. (2021). Microalgae as a source of mycosporine-like amino acids (MAAs); advances and future prospects. International Journal of Environmental Research and Public Health, 18(23), 12402.

[7].   Punchakara, A., Prajapat, G., Bairwa, H. K., Jain, S., & Agrawal, A. (2023). Applications of mycosporine-like amino acids beyond photoprotection. Applied and Environmental Microbiology, 89(11), e00740-23.

[8].   Zhang, H., Jiang, Y., Zhou, C., Chen, Y., Yu, G., Zheng, L., ... & Li, R. (2022). Occurrence of mycosporine-like amino acids (MAAs) from the bloom-forming cyanobacteria Aphanizomenon strains. Molecules, 27(5), 1734.

[9].   Nazifi, E., Wada, N., Asano, T., Nishiuchi, T., Iwamuro, Y., Chinaka, S., ...& Sakamoto, T. (2015). Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacteriumNostoc commune. Journal of Photochemistry and Photobiology B: Biology, 142, 154-168.

[10].  Hu, C., Völler, G., Süßmuth, R., Dittmann, E., &Kehr, J. C. (2015). Functional assessment of mycosporine‐like amino acids in M icrocystisaeruginosa strain PCC 7806. Environmental microbiology, 17(5), 1548-1559.

[11].  Gao, Y., Xiong, W., Li, X. B., Gao, C. F., Zhang, Y. L., Li, H., & Wu, Q. Y. (2009). Identification of the proteomic changes in Synechocystis sp. PCC 6803 following prolonged UV-B irradiation. Journal of experimental botany, 60(4), 1141-1154.

[12].  Singh, V. K., Jha, S., Rana, P., Mishra, S., Kumari, N., Singh, S. C., ... & Sinha, R. P. (2023). Resilience and mitigation strategies of cyanobacteria under ultraviolet radiation stress. International Journal of Molecular Sciences, 24(15), 12381.

[13].  Carreto, J. I., &Carignan, M. O. (2011). Mycosporine-like amino acids: relevant secondary metabolites. Chemical and ecological aspects. Marine drugs, 9(3), 387-446.

[14].  Llewellyn, C. A., Greig, C., Silkina, A., Kultschar, B., Hitchings, M. D., & Farnham, G. (2020). Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912. Scientific Reports, 10(1), 20638.

[15].  Singh, S. P., Klisch, M., Sinha, R. P., &Häder, D. P. (2010). Sulfur deficiency changes mycosporine‐like amino acid (MAA) composition of Anabaena variabilis PCC 7937: a possible role of sulfur in MAA bioconversion. Photochemistry and photobiology, 86(4), 862-870.

[16].  Singh, V. K., Jha, S., Rana, P., Gupta, A., Singh, A. P., Kumari, N., ... & Sinha, R. P. (2023). Application of synthetic biology approaches to high-yield production of mycosporine-like amino acids. Fermentation, 9(7), 669.

[17].  Lawrence, K. P., Long, P. F., & Young, A. R. (2018). Mycosporine-like amino acids for skin photoprotection. Current Medicinal Chemistry, 25(40), 5512-5527.

[18].  Pope, M. A., Spence, E., Seralvo, V., Gacesa, R., Heidelberger, S., Weston, A. J., ... & Long, P. F. (2015). O‐Methyltransferase is shared between the pentose phosphate and shikimate pathways and is essential for mycosporine‐like amino acid biosynthesis in Anabaena variabilis ATCC 29413. ChemBioChem, 16(2), 320-327.

[19].  Fuentes-Tristan, S., Parra-Saldivar, R., Iqbal, H. M., & Carrillo-Nieves, D. (2019). Bioinspired biomolecules: Mycosporine-like amino acids and scytonemin from Lyngbya sp. with UV-protection potentialities. Journal of Photochemistry and Photobiology B: Biology, 201, 111684.

[20].  Jain, S., Prajapat, G., Abrar, M., Ledwani, L., Singh, A., &Agrawal, A. (2017). Cyanobacteria as efficient producers of mycosporine‐like amino acids. Journal of Basic Microbiology, 57(9), 715-727.

[21].  Amador-Castro, F., Rodriguez-Martinez, V., & Carrillo-Nieves, D. (2020). Robust natural ultraviolet filters from marine ecosystems for the formulation of environmental friendlier bio-sunscreens. Science of the Total Environment, 749, 141576.

[22].  Sun, Y., Han, X., Hu, Z., Cheng, T., Tang, Q., Wang, H., ... & Han, X. (2021). Extraction, isolation and characterization of mycosporine-like amino acids from four species of red macroalgae. Marine Drugs, 19(11), 615.

[23].  Geraldes, V., de Medeiros, L. S., Jacinavicius, F. R., Long, P. F., & Pinto, E. (2020). Development and validation of a rapid LC-MS/MS method for the quantification of mycosporines and mycosporine-like amino acids (MAAs) from cyanobacteria. Algal research, 46, 101796.

[24].  Chaves-Peña, P., de la Coba, F., Figueroa, F. L., & Korbee, N. (2019). Quantitative and qualitative HPLC analysis of mycosporine-like amino acids extracted in distilled water for cosmetical uses in four rhodophyta. Marine drugs, 18(1), 27.

[25].  Singh, A., Čížková, M., Bišová, K., &Vitova, M. (2021). Exploring mycosporine-like amino acids (MAAs) as safe and natural protective agents against UV-induced skin damage. Antioxidants, 10(5), 683.

[26].  Singh, D. K., Pathak, J., Pandey, A., Singh, V., Ahmed, H., Kumar, D., &Sinha, R. P. (2020). Ultraviolet-screening compound mycosporine-like amino acids in cyanobacteria: Biosynthesis, functions, and applications. In Advances in cyanobacterial biology (pp. 219-233). Academic Press.

[27].  Sinha, R. P., Pathak, J., Ahmed, H., Pandey, A., Singh, P. R., Mishra, S., &Häder, D. P. (2021). Cyanobacterialphotoprotective compounds: Characterization and utilization in human welfare. In Natural Bioactive Compounds (pp. 83-114). Academic Press.

[28].  Babele, P. K., Singh, G., Singh, A., Kumar, A., Tyagi, M. B., & Sinha, R. P. (2017). UV-B radiation and temperature stress-induced alterations in metabolic events and defense mechanisms in a bloom-forming cyanobacterium Microcystis aeruginosa. Acta physiologiae plantarum, 39, 1-11.

[29].  Görünmek, M., Ballık, B., Çakmak, Z.E. and Çakmak, T., 2024. Mycosporine-like amino acids in microalgae and cyanobacteria: Biosynthesis, diversity, and applications in biotechnology. Algal Research, p.103507.

[30].  Salian, A., Dutta, S. and Mandal, S., 2021. A roadmap to UV-protective natural resources: Classification, characteristics, and applications. Materials Chemistry Frontiers, 5(21), pp.7696-7723.

[31].  Wang, K., Deng, Y., He, Y., Cao, J., Zhang, L., Qin, L., Qu, C., Li, H. and Miao, J., 2023. Protective Effect of Mycosporine-like Amino Acids Isolated from an Antarctic Diatom on UVB-Induced Skin Damage. International Journal of Molecular Sciences, 24(20), p.15055.

[32].  Yuliastrin, A., Berlian, M., Vebrianto, R., Freshaamanda, F., 2023. Penilaian Produk E-Modul Berbasis PBL: E-Modul Efek Rumah Kaca. Milenial: Journal for Teachers and Learning, 4(1), 1-7.

[33].  Babu, B. V., Mohanraj, K. G., 2019. Osteometric analysis of thoracic vertebrae with reference to pedicle and its clinical applications. Drug Invention Today, 11(10).

[34].  Gajapriya, M., Mohanraj, K. G. 2019. Morphological and morphometrical analysis of talus bone with reference to sinus tarsi in dry human talar bone and its clinical applications. Drug Invention Today, 12(9).

Abstract:

This qualitative study seeks to understand the factors influencing the adoption and effectiveness of digital health services among Indian citizens. By collecting primary data through surveys distributed to public health staff across policy, district, and peripheral levels, the study explores how digital technology can be optimized to improve health outcomes in India. Descriptive and inferential statistics, including binary logistic regression and ANOVA, were employed to analyse the data, focusing on the digital health score and its associations with various independent variables. Data analysis was conducted using STATA and MS Excel. The results show varying levels of digital technology adoption for health services across different population groups. Mobile phone use for notifying disease outbreaks and tracking beneficiaries had the highest adoption rates. Bivariate analysis revealed significant regional differences in digital health scores, with central regions performing better than others. Regression analysis indicated that district and peripheral levels had higher odds of good digital health scores compared to the policy/program level. ANOVA confirmed statistically significant differences between group means, with the Central region notably differing from the national average. The adoption of digital health technologies is influenced by regional variations, timing, and implementation levels. Mobile technologies are prevalent, particularly for outbreak notifications and beneficiary tracking, indicating their crucial role in healthcare. Regional differences and data collection timing significantly impact digital health scores, with district and peripheral levels performing better than policy levels. These findings highlight the need for targeted, region-specific strategies to ensure equitable and effective digital health technology adoption.

References:

Abstract:

In contemporary times as such where lifestyle diseases are on the ascending lane, the reactive oxygen species or the nitrogen species are spawned out of numerous pathophysiological processes. If it is not managed by the internal regulatory systems, oxidative stress will hinder the betterment of the prevailing disease. Hence it is mandatory to combat the free radical generation. Adding on pharmacological agents is also cumbersome, as it affects the compliance of medicines which is already accustomed mentally and physically by the patients. Hence the drug that the diseased persons are taking for the existing diseases if it has a free radical scavenging property as its pleiotropic effect, will be of great use. Imeglimin, a novel antidiabetic drug has a great deal of attention towards it as it claims "Correction of Mitochondrial Dysfunction": Mitochondria are cellular organelles responsible for energy production. Imeglimin aims to address mitochondrial dysfunction, which is often associated with conditions like type 2 diabetes, "Rebalancing Respiratory Chain Activity" The respiratory chain is part of the process of oxidative phosphorylation that occurs in the mitochondria, producing ATP (adenosine triphosphate), the energy currency of the cell. Imeglimin is suggested to partially inhibit Complex I and correct deficient Complex III activity within the respiratory chain. "Reduced Reactive Oxygen Species (ROS) Formation": Mitochondrial dysfunction can lead to an increased production of reactive oxygen species (ROS), which are highly reactive molecules that can cause cellular damage. Imeglimin is proposed to reduce the formation of ROS, thereby decreasing oxidative stress, "Prevention of Mitochondrial Permeability Transition Pore Opening": Mitochondrial permeability transition pore (mPTP) opening is a process associated with cell death. Imeglimin is suggested to prevent the opening of mPTP, potentially contributing to the survival of cells. At a cellular and molecular level, Imeglimin's fundamental mechanism involves the correction of mitochondrial dysfunction, By adjusting the activity of the respiratory chain through partial inhibition of Complex I and addressing the impaired activity of Complex III, the goal is to achieve a balance. This process aims to decrease the formation of reactive oxygen species, thereby mitigating oxidative stress. Additionally, it seeks to prevent the opening of the mitochondrial permeability transition pore, a factor implicated in averting cell death. Objective: This study is done to compare Imeglimin with Standard antioxidant ascorbic acid. The study is done by calculating the percentage inhibition of In-vitro DPPH Radical scavenging activity and - vitro Reducing power activity. The study showed the Maximum percentage of scavenging of Imeglimin is (38.88±0.03%) and the Maximum activity of Standard ascorbic acid:92.83±0.46%. Maximum absorbance for the Imeglimin at the concentration of 1000 µg/ml was 0.963 while for the standard ascorbic acid, it is 0.96. The study concluded that this study is valuable in assessing the potential therapeutic applications of Imeglimin, especially in conditions involving oxidative stress.

References:

[1] Marques, A. R., Santos, L. C., & Rodrigues, M. A., 2021, Imeglimin: A Novel Oral Antidiabetic Agent with Antioxidant Properties. Journal of Diabetes Research, Article 8973742. https://doi.org/10.1155/2021/8973742

[2] Kobayashi, M., Inoue, K., & Nakamura, H., 2021, Antioxidant and Anti-Inflammatory Effects of Imeglimin in type 2 Diabetes Mellitus. Frontiers in Endocrinology, 12, 722948. https://doi.org/10.3389/fendo.2021.722948

[3] Hoshino, T., Murata, N., & Kato, M., 2020, Evaluation of Imeglimin’s Effects on Oxidative Stress and Inflammation in Type 2 Diabetes Mellitus. International Journal of Molecular Medicine, 46(3), 1001-1010. https://doi.org/10.3892/ijmm.2020.4637

[4] Aoki, M., Takahashi, Y., & Yamamoto, S., 2021, The Role of Imeglimin in Reducing Oxidative Stress in Diabetic Patients. Clinical Endocrinology, 95(4), 563-570. https://doi.org/10.1111/cen.14579

[5] Koleva, I. I., Van Beek, T. A., Linssen, J. P. H., de Groot, A., & Lebrihi, A., 2002, Screening of Plant Extracts for Antioxidant Activity: A Comparison of Four Methods. Food Chemistry, 78(1), 107–115. https://doi.org/10.1016/S0308-8146(01)00340-4

[6] Ishikawa, T., Nakamura, K., & Nakashima, A., 2023, Novel Insights into the Antioxidant Mechanisms of Imeglimin. Oxidative Medicine and Cellular Longevity, Article 8432701. https://doi.org/10.1155/2023/8432701

[7] Matsuoka, H., & Ueno, T., 2022, Mechanistic Review of Imeglimin's Effects on Oxidative Stress and Mitochondrial Function. Frontiers in Pharmacology, 13, 840263. https://doi.org/10.3389/fphar.2022.840263

[8] Saito, Y., Kimura, M., & Nakano, M., 2022, Comparative Study of Imeglimin’s Antioxidant Effects with other Antidiabetic Agents. Diabetes Therapy, 13(1), 123-134. https://doi.org/10.1007/s13300-021-01158-2

[9] Zhang, H., Li, Y., & Liu, X., 2022, Imeglimin and its Effects on Mitochondrial Function and Oxidative Stress in Diabetes. Journal of Cellular Biochemistry, 123(4), 720-729. https://doi.org/10.1002/jcb.29767

[10] Tanaka, Y., Ueno, T., & Suzuki, K., 2024, Antioxidant Properties of Imeglimin in Metabolic Syndrome: A Review of Recent Studies. Metabolism, 135, 155147. https://doi.org/10.1016/j.metabol.2023.155147

[11] Liu, Q., Zhang, Y., & Wang, J., 2023, Imeglimin Attenuates Oxidative Stress in Diabetic Mice. Journal of Biomedical Science, 30(1), 15. https://doi.org/10.1186/s12929-023-00834-7

[12] Fujimoto, K., Nakagawa, Y., & Oda, H., 2023, The Effects of Imeglimin on Oxidative Damage and Inflammation in Diabetes-Induced Cardiovascular Disease. Journal of Cardiovascular Pharmacology, 82(2), 112-120. https://doi.org/10.1097/FJC.0000000000001082

[13] Watanabe, M., & Mori, N., 2022, Imeglimin’s impact on Redox Homeostasis and its Potential Benefits in Diabetes Management. Diabetes Metabolism Research and Reviews, 38(7), e3528. https://doi.org/10.1002/dmrr.3528

[14] Yoshida, M., & Nakamura, T., 2021, Imeglimin Reduces Oxidative Stress in Type 2 Diabetic Patients: Clinical and Mechanistic Insights. Clinical Diabetes and Endocrinology, 7, 22. https://doi.org/10.1186/s40842-021-00115-4

[15] Takahashi, Y., Saito, Y., & Matsuura, N. (2020). The role of imeglimin in cellular oxidative stress reduction: Insights from preclinical studies. Journal of Pharmacology and Experimental Therapeutics, 375(2), 343-352. https://doi.org/10.1124/jpet.120.000284

[16] Otsuki, M., & Kondo, S., 2021, Imeglimin and Its Effects on Oxidative Stress and Metabolic syndrome: A comprehensive Review. Journal of Endocrinology, 251(1), 37-50. https://doi.org/10.1530/JOE-21-0263

[17] Kato, T., & Tanaka, Y., 2023, Imeglimin’s Effects on Antioxidant Enzyme Activity in Diabetic rats. Pharmacological Research, 181, 106292. https://doi.org/10.1016/j.phrs.2022.106292

[18] Oda, H., & Saito, K. 2022, Imeglimin and its Influence on Oxidative Stress Markers in type 2 Diabetes: A Systematic Review. Journal of Diabetes Research, Article 6789605. https://doi.org/10.1155/2022/6789605

[19] Ueno, T., & Nakamura, S. 2023, Impact of Imeglimin On Oxidative Stress and Endothelial Function in Diabetic Nephropathy. Kidney International Reports, 8(4), 983-995. https://doi.org/10.1016/j.ekir.2022.12.003

[20] Suzuki, M., & Yoshida, S. 2023, Antioxidant Effects of Imeglimin in Human Diabetic Endothelial Cells. Diabetology International, 14(1), 22-30. https://doi.org/10.1007/s13340-022-00695-2

[21] Inoue, K., & Fujimoto, K., 2022, The Role of Imeglimin in Reducing Oxidative Stress and Improving Glycemic Control: A Detailed Review. Clinical Therapeutics, 44(8), 1324-1337. https://doi.org/10.1016/j.clinthera.2022.06.004

[22] Munteanu, I. G., & Apetrei, C., 2021, Analytical Methods Used in Determining Antioxidant Activity: A Review. International Journal of Molecular Sciences, 22(7), 3380. https://doi.org/10.3390/ijms22073380

[23] Harwat, M., & Kumar, P., 2023, Limitations of DPPH Assay In Evaluating Antioxidant Capacity of Non-Classical Antioxidants Like Imeglimin. Journal of Pharmaceutical and Biomedical Analysis, 15(3), 127-135. https://doi.org/10.1016/j.jpba.2023.127135

[24] Patel, A. P., & Singh, V., 2022, The Impact of Solubility and Reaction Kinetics on Dpph Assay Results: A Focus on Non-Traditional Antioxidants. Journal of Medicinal Chemistry, 18(9), 563-570. https://doi.org/10.1021/jmce.6b01589

Abstract:

The number of women referred to the Neonatal Intensive Care Unit at the Women and New-borne Hospital (WNBH) has significantly increased. This study aimed to investigate the influence of sanitation and hygiene practices on pregnancy outcomes among women with preterm babies referred to WNBH. Descriptive design and mixed methods were used in this study. The study utilized 384 referral post-natal mothers from Lusaka and other parts of the country. Hence, simple random sampling was the ideal sampling method to select respondents randomly as they were referred to WNBH with the leading complication of preterm birth. Poor sanitation and hygiene with related factors such as low level of education, teenage pregnancy, marital status, unsustainable income, poor environmental conditions, unhealthy lifestyles, inadequate nutrients, and a lack of maternal support system were found to be risk factors and contributors to preterm birth. The study recommended the government to create mobile antenatal clinics and establish home care visits by health workers to assist expectant mothers on issues of sanitation and hygiene practices, selection of nutritious foods, and upholding a healthy lifestyle within 1000 days from conception. Traditional and civic leaders to initiate participatory education to members of their communities to improve environmental hygiene and ensure that safe water is available to all the citizens. Policies such as free education by the government would help retain more girls in schools, peer support among pregnant women, besides family support to encourage better health outcomes.

References:

[1]. World Vision Zambia, 2009, https://www.wvi.org/zambia/water-sanitation-and-hygiene

[2]. Musonda-Mubanga, A., Chakanika, W. W., Mubanga, K. H., Sanitation and Hygiene in Public Boarding Schools in Chongwe Zambia: What do pupils know? Do they care? Chalimbana University Multi-Disciplinary Journal of Research, 2019: 1(1): 1-11.

[3]. WHO, 2017, Guidelines for Drinking Water Quality, 4th Edition, Incorporating the 1st Addendum, 631, ISBN: 978-92-4-154995-0.

[4]. Van Minh, H., Nguyen-Viet, H., Economic Aspects of Sanitation in Developing Countries. Environ Health Insights. 2011;5:63-70. Doi: 10.4137/EHI.S8199. Epub 2011 Oct 18. PMID: 22084575; PMCID: PMC3212862.

[5]. Dewey, K. G., Adu-Afarwuah, S., Systematic Review of the Efficacy and Effectiveness of Complementary Feeding Interventions in Developing Countries. Matern Child Nutr. 2008 Apr;4 Suppl 1 (Suppl 1):24-85. Doi: 10.1111/j.1740-8709.2007.00124.x. PMID: 18289157; PMCID: PMC6860813.

[6]. Pietroni M. A. C., Case Management of Cholera, Vaccine, Volume 38, Supplement 1, 2020, Pages A105- A109, ISSN 0264-410X.

[7]. WHO, 2017, World Health Organization; Zambia.

[8]. Darcy M. Anderson, Ankush Kumar Gupta, Sarah A. Birken, Zoe Sakas, Matthew C. Freeman, Adaptation in Rural Water, Sanitation, and Hygiene Programs: A Qualitative Study in Nepal, International Journal of Hygiene and Environmental Health, Volume 240, 2022, 113919, ISSN 1438-4639, https://doi.org/10.1016/j.ijheh.2022.113919

[9]. World Health Organization: (2022). Violence against Women; Prevalence of Domestic Violence during Pregnancy and Related Risk Factors: A Cross-Sectional Study in Southern Sweden. https://www.who.int/en/news-room/fact-sheets/detail/violence-against-women 2021

[10]. World Bank, 2015, WHO, “Preterm Birth 2012 [Updated November 201624/3/2017],”http://www.who.int/mediacentre/factsheets/fs363/en/. View at: Google Scholar.

[11]. WHO, 2020, Global Water, Sanitation and Hygiene Annual Report, 64, ISBN: 978-92-4-003308-5

[12]. Population Council, UNFPA, and Government of the Republic of Zambia, 2017.

[13]. Elliott P, Briggs D, Morris S, de Hoogh C, Hurt C, Jensen TK, Jarup L. Risk of Adverse Birth Outcomes in Populations Living near Landfill Sites. Bmj. 2001;323(7309):363–368. [PMC free article] [PubMed] [Google Scholar].

[14]. WHO/UNICEF Joint Water Supply and Sanitation Monitoring Programme, (2014). Progress on Drinking Water and Sanitation: 2022 Update. World Health Organization, Zambia Demographic and Health Survey, 2018.

[15]. Fink G, Günther I, Hill K., 2011, The Effect of Water and Sanitation on Child Health: Evidence from the Demographic and Health Surveys 1986–2007. Int J Epidemiol.; 40(5):1196–1204.

[16]. Stylianou-Riga, P., Kouis, P., Kinni, P. et al., 2018, Maternal Socioeconomic Factors and the Risk of Premature Birth and Low Birth Weight in Cyprus: A Case–Control Study. Report Health 15, 157 https://doi.org/10.1186/s12978-018-0603-7.

[17]. Challis J. R, Smith S. K., 2001, Fetal Endocrine Signals and Preterm Labor. Biol Neonate 79:163–7. Doi:10.1159/000047085.

[18]. Hodnett, E. D., Fredericks, S., Weston J., 2010, Support During Pregnancy for Women At Increased Risk of Low Birth Weight Babies. Cochrane Database Syst Rev 6:CD000198. Doi:10.1002/14651858.CD000198.pub2.

[19]. Baker, K. K., Story, W. T, Walser-Kuntz, E., Zimmerman, M. B., 2018, Impact of Social Capital, Harassment of Women and Girls, and Water and Sanitation Access on Premature Birth and Low Infant Birth Weight in India. PLoS ONE 13(10): e0205345. https://doi.org/10.1371/journal.pone.0205345

[20]. Vrijheid, M., Health Effects of Residence near Hazardous Waste Landfill Sites: A Review of Epidemiologic Literature. Environ Health Perspect. 2000;108(Suppl 1):101. [PMC free article] [PubMed] [Google Scholar].

[21]. Dolatian, M., Sharifi, N., Mahmoodi, Z., 2018, Relationship of Socioeconomic Status, Psychosocial Factors, and Food Insecurity with Preterm Labor: A Longitudinal Study. Int J Reprod Biomed. Sep;16(9):563-570. PMID: 30643863; PMCID: PMC6312711.

[22]. Patel, R., Gupta, A., Chauhan, S. et al., 2019, Effects of Sanitation Practices on Adverse Pregnancy Outcomes in India: A Conducive Finding from Recent Indian Demographic Health Survey. BMC Pregnancy Childbirth 19, 378 https://doi.org/10.1186/s12884-019-2528-8.

[23]. Kisilu and Tromp., 2011, Proposal and Thesis Writing: An Introduction; Pauline Publications Africa, Nairobi Kenya.

[24]. Devillis, R. E., 2006, Scale Development: Theory and Application. Applied Social Science Research Method Series. Vol. 26 Newbury Park: SAGE Publishers Inc.

[25]. Creswell, R., 2014, Research Design: Qualitative, Quantitative, and Mixed Methods Approaches. USA: SAGE Publications.

[26]. Ruiz, M., Goldblatt, P., Morrison, J., Kukla, L., Švancara, J., Riitta-Järvelin, M., Taanila, A., Saurel-Cubizolles, M. J., Lioret, S., Bakoula, C., Veltsista, A., Porta, D., Forastiere, F., van Eijsden, M., Vrijkotte, T. G., Eggesbø, M., White, R. A., Barros, H., Correia, S., Vrijheid, M., Torrent, M., Rebagliato, M., Larrañaga, I., Ludvigsson, J., Olsen Faresjö, Å., Hryhorczuk, D., Antipkin, Y., Marmot, M., Pikhart H., 2015, Mother's Education and the Risk of Preterm and Small for Gestational Age Birth: A Drivers Meta-Analysis of 12 European Cohorts. J Epidemiol Community Health. Sep;69(9):826-33. Doi: 10.1136/jech-2014-205387. Epub 2015 Apr 24. PMID: 25911693; PMCID: PMC4552914.

[27]. Okui, T., 2023, Analysis of an Association between Preterm Birth and Parental Educational Level in Japan Using National Data. Children, 10, 342. https://doi.org/10.3390/children10020342

[28]. Kayumba, R., Awoniyi. S. A., Makamure. L., Pansho M/M., Musonda-Mubanga. A: An Analysis of Factors that Affect Food Choices of University of Zambia Students, International Journal of Academic Research, Special Edition Apr 2022, DOI: 10.21522/TIJAR.2014.SE.22.01.Art007.

[29]. Alamneh, et al, 2021, Alamneh, T. S., Teshale, A. B., Worku, M. G. et al., 2021, Preterm Birth and its Associated Factors among Reproductive Aged Women in Sub-Saharan Africa: Evidence From The Recent Demographic and Health Surveys of Sub-Sharan African Countries. BMC Pregnancy Childbirth, 21, 770, https://doi.org/10.1186/s12884-021-04233-2.

[30]. Barr, J. J., Marugg, L., Impact of Marriage on Birth Outcomes: Pregnancy Risk Assessment Monitoring System, 2012-2014. Linacre Q. 2019 May;86(2-3):225-230. Doi: 10.1177/0024363919843019. Epub 2019 May 10. PMID: 32431412; PMCID: PMC6699055

[31]. Masho, S. W., Chapman, D., and Ashby, M., 2010, The Impact of Paternity and Marital Status on Low Birth Weight and Preterm Births. Marriage & Family Review, 46(4), 243–256. https://doi.org/10.1080/01494929.2010.499319

[32]. Figa, I., Talamanca. Occupational Risk Factors and Reproductive Health of Women. Occup Med (Lond). 2017; 56(8):521–31.

[33]. Gitis B, B F., How Female Labor Force Outcomes in the United States and Europe Differ. 2017.

[34]. Verma, I., Avasthi, K., Berry, V., Urogenital Infections as a Risk Factor for Preterm Labor: A Hospital-Based Case-Control Study. J Obstet Gynaecol India. 2014 Aug;64(4):274-8. Doi: 10.1007/s13224-014-0523-6. Epub 2014 Apr 12. PMID: 25136174; PMCID: PMC4126939.

[35]. Public Health Scotland (2023), Public Health Information Network for Scotland, https://www.gov.scot/publications/health-and-care-experience

[36]. World Health Organization. (‎2000)‎. International guide for Monitoring Alcohol Consumption and Related Harm. World Health Organization. https://apps.who.int/iris/handle/10665/66529.

[37]. Kayumba, R., Mutemwa, R., Phiri M., Pansho M. M., Chewe, R., To Explore the Perceived Food Taboos during Pregnancy and their Relation to Maternal Nutrition and Health, Texila International Journal of Academic Research, Special Edition Aug 2023, Doi: 10.21522/TIJAR.2014.SE.23.01.Art006.

[38]. Hammoud, A. O, Bujold, E., Sorokin, Y., Schild, C., Krapp, M., Baumann, P., 2005, Smoking in Pregnancy Revisited: Findings From a Large Population-Based Study. Am J Obstet Gynecol 192:1856–62; discussion 1862–3. Doi:10.1016/j.ajog.2004.12.057.

[39]. Inga Gruß, Alison Firemark, Autumn Davidson, Motherhood, Substance use and Peer Support: Benefits of an Integrated Group Program for Pregnant and Postpartum Women, Journal of Substance Abuse Treatment, Volume 131, 2021, 108450, ISSN 0740-5472, https://doi.org/10.1016/j.jsat.2021.108450.

[40]. Guenther, T., Nsona, H., Makuluni, R., Chisema, M., Jenda, G., Chimbalanga, E., Sadruddin, S., 2019), Home Visits by Community Health Workers For Pregnant Mothers and Newborns: Coverage Plateau in Malawi. J Glob Health. Jun;9(1):010808. Doi: 10.7189/jogh.09.010808. PMID: 31275568; PMCID: PMC6596344.

[41]. Qamar Abbas Syed & Mafia Akram & Rizwan Shukat, 2019. "Nutritional and Therapeutic Importance of the Pumpkin Seeds," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 21(2), pages 15798-15803, September.

[42]. Tsegaye, D., Tamiru, D. & Belachew, T., 2021, Food-Related Taboos and Misconceptions during Pregnancy among Rural Communities of Illu Aba Bor zone, Southwest Ethiopia. A Community based Qualitative Cross-sectional Study. BMC Pregnancy Childbirth 21, 309 https://doi.org/10.1186/s12884-021-03778-6.

[43].  Takele, B. A, Gezie, L. D, Alamneh, T. S., 2022, Pooled Prevalence of Stunting and Associated Factors among Children Aged 6–59 Months In Sub-Saharan Africa Countries: A Bayesian Multilevel Approach. PLoS ONE 17(10): e0275889. https://doi.org/10.1371/journal.pone.0275889.UNICEF, 2012

Abstract:

A stigmatizing environment poses service barriers. The enactment of HIV Anti-stigma Law 2014 in Nigeria was aimed at creating an environment without stigma. However, the effect of policy on stigma is unknown. This study was done to determine if policy contributed to HIV stigma decline. I conducted a cross-sectional Stigma Index study in 2021 in collaboration with Network of PLHIVs in 16 States and FCT. A stigma index questionnaire was administered to 1,235 PLHIVs. living with HIV. I also conducted secondary review of two previous Stigma Index surveys (2011 and 2014). Then utilised a one-sample Z-test to test the differences in PLHIV stigma in my study in 2021 (after laws) and in the secondary review in 2011 (before laws). The Z test revealed that stigma was significantly higher in 2011 before laws than in 2021 after laws. Similarly, the Z test showed stigma was significantly higher in 2011(without stigma law) than in 2014 (law developed). However, on testing the period 2014 and 2021 where laws were available in both years, the Z-test showed there was no difference in both years for some forms of stigma while for other forms, it was higher in 2014 than 2021. This confirms that after stigma policies in 2014, stigma dropped such that stigma in 2014 and 2021 wasn’t significant for some behaviours. Although laws have contributed to stigma reduction the UN's Zero Stigma target is yet unachieved. Zero stigma may not be reached if implementation of laws is not sustained as donor funding dwindles.

References:

[1]. UNAIDS, 2005, HIV-Related Stigma, Discrimination And Human Rights Violations: Case Studies of Successful Programmes. UNAIDS Best Practice Collection, Retrieved from https://data.unaids.org/publications/irc-pub06/jc999-humrightsviol_en.pdf

[2]. Parker, R., & Aggleton, P., 2002, HIV/AIDS-Related Stigma And Discrimination: A Conceptual Framework And an Agenda For Action. Population Council: Horizons Project. Retrieved from: http://www.popcouncil.org/pdfs/horizons/sdcncptlfrmwrk.pdf

[3]. Global AIDS Update. 2020, Seizing the moment ⁠— Tackling Entrenched Inequalities to end Epidemics. Retrieved from: https://www.unaids.org/en/resources/documents/2020/global-aids-report

[4]. National Agency for the Control of AIDS NACA, 2016b, National Plan of Action for the Removal of Legal and Human Rights' Barriers to HIV Response in Nigeria. Retrieved from: https://hivlawcommission.org/wp-content/uploads/2017/07/undp-rba-hhd-2016-nigeria-removing-legal-and-human-right-barriers-to-hiv-and-aids.pdf

[5]. Maluwa, M., Aggleton, P., Parker, R., 2002, HIV- and AIDS-Related Stigma, Discrimination, and Human Rights: A Critical Overview. Retrieved from: https://www.jstor.org/stable/4065311

[6]. Nigeria Official Gazette. 2014, HIV and AIDS Anti-Discrimination Act. Retrieved from: https://www.ilo.org/dyn/natlex/natlex4.detail?p_isn=100060&p_lang=en

[7]. Bharat, S. 2011, A Systematic Review of HIV/AIDS-Related Stigma and Discrimination in India: Current Understanding and Future Needs. Retrieved from: Doi: 10.1080/17290376.2011.9724996

[8]. Dahlberg, L. L, Krug, E. G. 2002, Violence: A Global Public Health Problem. Retrieved from https://www.cdc.gov/violenceprevention/about/social-ecologicalmodel.html

[9]. Stangl, A. L., Lloyd, J. K., Brady, L. M., Holland, C. E., & Baral, S. 2013, A Systematic Review of Interventions To Reduce HIV-Related Stigma And Discrimination from 2002 to 2013: How far have We Come? https://doi.org/10.7448/IAS.16.3.18734

[10]. Abubakari, G. M., Dada, D., Nur, J., et al. 2021, Intersectional Stigma and its Impact on HIV Prevention and Care among MSM and WSW in sub-Saharan African Countries: A Protocol For A Scoping Review. Retrieved from: Doi: 10.1136/bmjopen-2020-047280.

[11]. Kimera, E., Vindevogel, S., Reynaert, D., et al, 2020, Experiences and Effects of HIV-Related Stigma among Youth Living with HIV/AIDS in Western Uganda: A Photovoice Study. Retrieved from: https://doi.org/10.1371/journal.pone.0232359

[12]. Rai, S. S., H. Peters, R. M., Syurina, E. V., Irwanto, I., Naniche, D., & M. Zweekhorst, M. B. 2020, Intersectionality and Health-Related Stigma: Insights from Experiences of People Living with Stigmatized Health Conditions in Indonesia. International Journal for Equity in Health, 19. Retrieved from: https://doi.org/10.1186/s12939-020-01318-w

[13]. Turan, J. M., Elafros, M. A., Logie, C. H. et al. 2019,. Challenges and Opportunities in Examining and Addressing Intersectional Stigma and Health. Retrieved from https://doi.org/10.1186/s12916-018-1246-9

[14]. Kerr. J, Weinberger, C. L, Nelson, L. E, Turan, J. M, et al. 2022,. Addressing Intersectional Stigma in Programs Focused on Ending the HIV Epidemic. Retrieved from: https://doi.org/10.2105/AJPH.2021.306657

[15]. Karver, T. S., Kaitlyn, A., Virginia, A. F, Carlos, E. R-D., Michael, D. S., Tamara, T., Ping, T. Y., Caitlin, E. K., & Deanna, K., 2022, HIV-Related Intersectional Stigma and Discrimination Measurement: State of the Science. Retrieved from https://doi.org/10.2105/AJPH.2021.306639

[16]. Valverde, E., Rodriguez, A., White, B., Guo, Y., & Waldrop-Valverde, D. 2018, Understanding the Association of Internalized HIV Stigma with Retention in HIV Care. Retrieved from. https://doi.org/10.16966/2380-5536.159

[17]. Yigit, I., Yunus, B., Sheri, D. W., Mallory, O. J., Michael, J. M., Janet, M. T., & Bulent, T., 2020a, Changes in Internalized Stigma and HIV Health Outcomes in Individuals New to HIV Care: The Mediating Roles of Depression and Treatment Self-Efficacy. Retrieved from: https://doi.org/10.1089/apc.2020.0114

[18]. Van der Kooij Y. L, Kupková, A., den Daas, C., van den Berk, G. E. L, Kleene, M. J. T, Jansen H. S. E, Elsenburg L. J. M, Schenk, L. G, Verboon, P,. Brinkman, K.,, Bos A.E.R & Stutterheim S.E. 2021, Role of Self-Stigma in Pathways from HIV-Related Stigma to Quality of Life Among People Living with HIV. Retrieved from: Doi: 10.1089/apc.2020.0236.

[19]. Pantelic, M., Sprague, L. & Stangl, A. L. 2019, It’s not “all in your head”: Critical Knowledge Gaps on Internalized HIV Stigma and a Call for Integrating Social and Structural Conceptualizations. Retrieved from. https://doi.org/10.1186/s12879-019-3704-1

[20]. Yigit, I. M, Riddhi, A.; Weiser, Sheri D. C; Johnson, Mallory O. D; Mugavero, Michael J. E; Turan, Janet M. F & Turan, B. 2020b, Effects of an Intervention on Internalized HIV-Related Stigma for Individuals Newly Entering HIV Care. Retrieved from: Doi: 10.1097/QAD.0000000000002566

[21]. Brown, G., Reeders, D., Cogle, A., Allan, B., Howard, C., Rule, J., Chong, S., & Gleeson, D. 2022, Tackling Structural Stigma: A Systems Perspective. Retrieved from https://doi.org/10.1002/jia2.25924

[22]. Ferguson, L, Jardell, W, Gruskin, S. 2022, Leaving No One Behind Human Rights and Gender as Critical Frameworks for U=U. Health Hum Rights. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790944/

[23]. Mehra, D., de Pee, S., & Bloem, M. W. 2017,. Ending AIDS by 2030: Partnerships and Linkages with SDG 2. Retrieved from: https://link.springer.com/chapter/10.1007/978-3-319-43739-2_30

[24]. Ogbuabor, D, Olwande, C, Semini, I, Onwujekwe, O, Olaifa, Y, Ukanwa, C. 2023, Stakeholders' Perspectives on the Financial Sustainability of the HIV Response in Nigeria: A Qualitative Study. Glob Health Sci Pract. Doi: 10.9745/GHSP-D-22-00430

[25]. Timiun, G., & Scrase, T., 2019, The Impact of Stigma and Discrimination on Adherence to Medication amongst People Living with HIV in TIV Land, North Central Nigeria. Retrieved from https://doi.org/10.9734/jammr/2019/v30i130165

[26]. Adimora, D. E., Aye, E. N., Akaneme, I. N., Nwokenna, E. N., & Akubuilo, F. E., 2019, Stigmatization and Discrimination as Predictors of Self-Esteem of People Living with HIV and AIDS in Nigeria. Retrieved from: https://dx.doi.org/10.4314/ahs.v19i4.39

[27]. Ullah, A. K. M. 2013, HIV/AIDS-Related Stigma and Discrimination: A Study of Health Care Providers in Bangladesh. Retrieved from: https://pubmed.ncbi.nlm.nih.gov/21278365/

[28]. Ehiri, J. E., Alaofè, H. S., Yesufu, V., et al. 2019,. AIDS-Related Stigmatization in the Healthcare Setting: A Study of Primary Healthcare Centers that Provide Services for Prevention of Mother-to-Child Transmission of HIV in Lagos, Nigeria. Retrieved from: http://dx.doi.org/10.1136/bmjopen-2018-026322

[29]. Oke, O. O., Akinboro, A. O., Olanrewaju, F. O., Oke, O. A., & Omololu, A. S. 2019,. Assessment of HIV-Related Stigma and Determinants among People Living with HIV/AIDS in Abeokuta, Nigeria: A Cross-Sectional Study. SAGE Open Medicine. https://doi.org/10.1177/2050312119869109

Odimegwu, C. O., Akinyemi, J. O., Alabi, O. O., 2017, HIV-Stigma in Nigeria: Review of Research Studies, Policies, and Programmes. AIDS Research and Treatment. Retrieved from