Piperine Attenuates Insulin Resistance in Adipocytes by Modulating the Expression of Proinflammatory Signaling Molecules in Experimental Diabetic Wistar Rats

Download Article

DOI: 10.21522/TIJPH.2013.SE.24.01.Art013

Authors : Gayathri R, Vishnu Priya Veeraraghavan, Kavitha. S, J. Selvaraj, Hema Shree K, Anuska Balaji


The development of insulin resistance and diabetes in obese people is caused by metabolic inflammation. In adipose tissues in the abdomen, macrophages infiltrate and cause insulin resistance. As a result of the macrophages adopting an M1 polarization, pro-inflammatory cytokines are released. Piperine, known for its potent anti-inflammatory properties, is being studied as a potential treatment for inflammatory diseases. Our findings suggest that piperine, an immunomodulator, can effectively treat obesity-related diabetes.


[1] Matafome P, Seiça R (2017) Function and Dysfunction of Adipose Tissue. Obesity and Brain Function 3–31.

[2] Gray SL, Vidal-Puig AJ (2007) Adipose Tissue Expandability in the Maintenance of Metabolic Homeostasis. Nutr Rev 65:S7–S12.

[3] Roy PK, Islam J, Lalhlenmawia H (2023) Prospects of potential adipokines as therapeutic agents in obesity-linked atherogenic dyslipidemia and insulin resistance. Egypt Heart J 75:24.

[4] Suwana J, Mahidon M, Mahāwitthayālai Mahidon. Faculty of Science (2005) Effects of Piperine on Wistar Rats.

[5] BrahmaNaidu P, Nemani H, Meriga B, Mehar SK, Potana S, Ramgopalrao S (2014) Mitigating efficacy of piperine in the physiological derangements of high fat diet induced obesity in Sprague Dawley rats. Chem Biol Interact 221:42–51.

[6] Vm AE (2019) Epidemics and Pandemics in India throughout History: A Review Article. Indian Journal of Public Health Research & Development.

[7] Sivakumar N, Geetha RV, Priya V (2021) Gayathri R, Dhanraj Ganapathy. Targeted Phytotherapy For Reactive Oxygen Species Linked Oral Cancer. Int J Dentistry Oral Sci 8:1425–1429.

[8] Nasim I, Rajesh Kumar S, Vishnupriya V, Jabin Z (2020) Cytotoxicity and anti-microbial analysis of silver and graphene oxide bio nanoparticles. Bioinformation 16:831.

[9] Tripathi AK, Ray AK, Mishra SK (2022) Molecular and pharmacological aspects of piperine as a potential molecule for disease prevention and management: evidence from clinical trials. Beni Suef Univ J Basic Appl Sci 11:16.

[10] Sama V, Nadipelli M, Yenumula P, Bommineni MR, Mullangi R (2012) Effect of piperine on antihyperglycemic activity and pharmacokinetic profile of nateglinide. Arzneimittelforschung 62:384–388.

[11] Pingale PL (2022) Drug Delivery Technology: Herbal Bioenhancers in Pharmaceuticals. Walter de Gruyter GmbH & Co KG.

[12] Vishaka S, Sridevi G, Selvaraj J (2022) An in vitro analysis on the antioxidant and anti-diabetic properties of Kaempferia galanga rhizome using different solvent systems. J Adv Pharm Technol Res 13:S505.

[13] Padmapriya A, Preetha S, Selvaraj J, Sridevi G (2022) Effect of Carica papaya seed extract on IL-6 and TNF-α in human lung cancer cell lines-an In vitro study. Research Journal of Pharmacy and Technology 15:5478–5482.

[14] Pratheebha C, Devi RG, Priya AJ EFFECT OF PEPPERMINT AND ROSEMARY OIL ON CONSTIPATION. Plant Cell Biotechnol. Mol. Biol.

[15] Knowledge about the effects of medicinal plants against COVID-19 among dental students-A questionnaire study.

[16] Lakshmi T Medicinal value and oral health aspects of acacia catechu-an update. Int. J. Dent. Oral Sci.

[17] Liu C, Yuan Y, Zhou J, Hu R, Ji L, Jiang G (2020) Piperine ameliorates insulin resistance via inhibiting metabolic inflammation in monosodium glutamate-treated obese mice. BMC Endocr Disord 20:152.

[18] Wang X, Zhang Y, Zhang L, Wang W, Che H, Zhang Y (2022) Piperine attenuates hepatic steatosis and insulin resistance in high-fat diet-induced obesity in Sprague-Dawley rats. Nutr Res 108:9–21.

[19] Woo H-M, Kang J-H, Kawada T, Yoo H, Sung M-K, Yu R (2007) Active spice-derived components can inhibit inflammatory responses of adipose tissue in obesity by suppressing inflammatory actions of macrophages and release of monocyte chemoattractant protein-1 from adipocytes. Life Sci 80:926–931.

[20] Dinda B (2022) Natural Products in Obesity and Diabetes: Therapeutic Potential and Role in Prevention and Treatment. Springer Nature.

[21] Koodathil J, Venkatachalam G, Bhaskaran K (2023) and antidiabetic activity of bitter honey in streptozotocin-nicotinamide-induced diabetic Wistar rats. J Med Life 16:91–100.

[22] Abijeth B, Ezhilarasan D (2020) Syringic acid induces apoptosis in human oral squamous carcinoma cells through mitochondrial pathway. J Oral Maxillofac Pathol 24:40–45.

[23] Heidari H, Bagherniya M, Majeed M, Sathyapalan T, Jamialahmadi T, Sahebkar A (2023) Curcumin-piperine co-supplementation and human health: A comprehensive review of preclinical and clinical studies. Phytother Res. https://doi.org/10.1002/ptr.7737.

[24] Kumar Seetharaman P, Ramalingam P, Chandrika M, Rajan R, Chelliah J, Bo L (2023) Antidiabetic potential of Gymnemic acid mediated gold nanoparticles (Gym@AuNPs) on Streptozotocin-induced diabetic rats-An implication on in vivo approach. Int J Pharm 636:122843.

[25] Muller CJF, de Beer D, Joubert E, Louw J, Gabuza K, Fey SJ (2011) Assessment of the Antidiabetic Potential of an Aqueous Extract of Honeybush (Cyclopia Intermedia) in Streptozotocin and Obese Insulin Resistant Wistar Rats.

[26] Kishore SOG, Priya AJ, Narayanan L Controlling of oral pathogens using turmeric and tulsi herbal formulation mediated copper nanoparticles. Plant Cell Biotechnol. Mol. Biol.

[27] Bhupatiraju L, Bethala K, Wen Goh K, et al (2023) Influence of extract on diabetes induced rat brain aging. J Med Life 16:307–316.

[28] Karthik EVG, Priya V (2021) Gayathri. R, Dhanraj Ganapathy. Health Benefits Of Annona Muricata-A Review. Int J Dentistry Oral Sci 8:2965–2967

[29] Priya DV, (2020) Knowledge and awareness on HIV/AIDS among college students in A university hospital setting. Int J Dent Oral Sci 1182–1186

[30]   Ganapathy D, (2021) Awareness of hazards caused by long-term usage of polyethylene terephthalate (PET) bottles. Int J Dent Oral Sci 2976–2980