Preliminary Phytochemical Analysis and Anti-Cholesterol Potential of Ethanolic Seed Extract of Illicium Verum

Download Article

DOI: 10.21522/TIJPH.2013.SE.24.01.Art024

Authors : Gayathri R, Vishnu Priya Veeraraghavan, Kavitha S, J. Selvaraj, Vaishali. B


Illicium Verum commonly called star anise, which is indigenous to northeast Vietnam and Southwest China. Illicium verum is one of the vital ingredients of Chinese medicinal herbs. Principal component (Trans-anethol) exhibits antimicrobial activity such as antiviral, anti-bacterial, antifungal and antiparasitic activities. Cholesterol, a waxy or fat like substance which is found in each and every cell of our body. Cholesterol helps our metabolism work efficiently, but if our body has too much cholesterol in blood, it causes several harmful effects. Anti-cholesterol drugs are useful in reducing the blood cholesterol level. Ethanolic extract of Illicium verum was found to be rich in phytochemicals such as Alkaloids, terpenoids and steroids. The ethanolic extract of illicium verum exhibited a significant antioxidant potential (Ic50 = 300 µg/ml) and anti-cholesterol potential (Ic50 = 400 µg/ml) as compared to standard drugs.


[1] Rocha L, Tietbohl LAC (2016). Staranise (Illicium verum Hook) Oils. Essential Oils in Food Preservation, Flavor and Safety 751–756.

[2] Lim TK (2013). Illicium verum. Edible Medicinal and Non-Medicinal Plants 151–160.

[3] Hussaini J, Faculty of Medicine, Microbiology Department, Mara UT, Buloh 47000 Sg, Selangor, Malaysia, Maziz MN (2012). Antiulcer and Antibacterial Evaluations of Illicium Verum Ethanolic Fruits Extract (Ivefe). International Journal of Scientific Research 2:410–412.

[4] Damayanti R, Tamrin, Eddyanto, Alfian Z (2019). Illicium verum Essential Oil as Antibacterial Agent. Proceedings of the 1st International Conference on Chemical Science and Technology Innovation.

[5] Yu C, Yang W, Jiang S, Wang T, Yang Z (2021). Effects of star anise (illicium verum hook.f.) essential oil administration under three different dietary energy levels on growth performance, nutrient, and energy utilization in broilers. Anim Sci J. 92: e13496.

[6] Gayathri V, Kiruba, D. (2014). Preliminary Phytochemical Analysis of Leaf Powder Extracts of Psidium Guajava L. Journal of Pharmacognosy and Phytochemical.

[7] Patil BR, Madhu CS, Manukumar HM, Tribhuvan (2014). Phytochemical, Nutritional and Mineral Constituents of Illicium Verum Hook (Star anise). World Journal of Pharmaceutical Research 3:2888–2896.

[8] Patra JK, Das G, Bose S, Banerjee S, Vishnuprasad CN, Rodriguez‐Torres MP, Shin H (2020). Star anise (Illicium verum): Chemical Compounds, Antiviral Properties, and Clinical Relevance. Phytotherapy Research 34:1248–1267.

[9] Shankar P, Gayathri R, Selvaraj J, Priya VV (2021). Antidiabetic and Antiinflammatory Potentials of Sida Acuta Leaf Ethanolic Extract. Journal of Pharmaceutical Research International 33:8.

[10] Jayaraman S, Priya VV, Gayathri R, Others (2023). Effect of Antioxidant Vitamins on Protein Kinase-C And Phosphotyrosine Phosphatase 1b Expression in The Liver of Glyphosate-Induced Experimental Diabetic Rats. Journal of Namibian Studies: History Politics Culture 33:5951–5962.

[11] Xu Z, Hileuskaya K, Kraskouski A, Yang Y, Huang Z, Zhao Z (2024) Inhibition of α-glucosidase activity and intestinal glucose transport to assess the in vivo anti-hyperglycemic potential of dodecyl-acylated phlorizin and polydatin derivatives. Food Funct.

[12] Banu KS, Cathrine L (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science 2:25–32.

[13] Subaraman M, Gayathri R, Priya VV (2020). In vitro antidiabetic activity of crude acetone leaf extract of Annona muricata. Drug Invention Today.

[14] Rosenfeld ME (2013). Inflammation and atherosclerosis: direct versus indirect mechanisms. Curr Opin Pharmacol 13:154–160.

[15] Vijayakumar K, Rengarajan RL, Radhakrishnan R, Vijaya Anand A (2018). Hypolipidemic Effect of Psidium guajava Leaf Extract Against Hepatotoxicity in Rats. Pharmacogn Mag 14:4.

[16] Harini P, Veeraraghavan VP, Selvaraj J, Gayathri R, Kavitha S (2022) Antidiabetic activity of Kabasura Kudineer Chooranam. J Adv Pharm Technol Res 13: S383–S386.

[17] Das MP, Selva Kumar S (2013) Preliminary Phytochemical Analysis of Illicium verum and Wedelia chinensis. International Journal of PharmTech Research 5:974–4304.

[18] Eveline, Eveline E, Novita A (2020) Antibacterial Potential of Star Anise (Illicium verum Hook. f.) Against Food Pathogen Bacteria. Microbiology Indonesia 14:3.

[19] Venkateshwaran V, Vishnu Priya V, Jayaraman S, Gayathri R, Kavitha S (2023) Role of Antioxidant Vitamins on the Expression on the Sparc, Munc. 18 and Syntaxin Mrna in the Gastrocnemius Muscle of Glyphosate Induced Experimental Rats. HIV AIDS 23:157–167.

[20] Ganapathy D, (2021) Awareness of hazards caused by long-term usage of polyethylene terephthalate (PET) bottles. Int J Dent Oral Sci 2976–2980

[21] Karthik EVG, Priya V (2021) Gayathri. R, Dhanraj Ganapathy. Health Benefits Of Annona Muricata-A Review. Int J Dentistry Oral Sci 8:2965–2967

[22] Ganesan A, Muthukrishnan A, Veeraraghavan V (2021) Effectiveness of Salivary Glucose in Diagnosing Gestational Diabetes Mellitus. Contemp Clin Dent 12:294–300