Comparative Analysis of Antimicrobial Efficacy: Harnessing Cocos Nucifera Extracts in Novel Toothpaste Formulations

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DOI: 10.21522/TIJPH.2013.SE.24.01.Art025

Authors : Aravind Kumar Subramanian, Nivethigaa Balakrishnan


Natural resources, ranging from herbs like neem and clove to minerals like fluoride, are harnessed for their antimicrobial properties in oral care products. These resources provide sustainable alternatives to synthetic chemicals, offering effective yet eco-friendly solutions against oral microorganisms. The present study aims to assess the antimicrobial efficacy of two toothpaste formulations derived from the extracts of Cocos nucifera. Two toothpaste preparations were made from the pulp extracts of Cocos nucifera. Group 1 was prepared from milk extracted from freshly ground coconut pulp. The second extract was obtained by the lyophilization process. Both toothpaste formulations were then analyzed for their antimicrobial efficacy by assessing the zone of inhibition for the following oral pathogens; Streptococcus mutans, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Lactobacillus rhamnosus, Candida albicans by agar well diffusion assay. Statistical analysis was performed using the SPSS software. It was found that the toothpaste derived from the extracts of coconut milk had antimicrobial efficacy against S.mutans, S.aureus and C.albicans in the various dilutions tested and the toothpaste made from the lyophilized extract exhibited antimicrobial efficacy against P.aeruginosa in various dilutions. Toothpaste derived from the coconut milk extract demonstrated significant efficacy against tested oral pathogens, suggesting its suitability for incorporation into oral care products. Its antimicrobial properties highlight its potential for combating oral infections and enhancing oral hygiene routines. Further research and development could optimize its utilization in various dental formulations.


[1] Asghar, Muhammad Tuseef, Yus Aniza Yusof, Mohd Noriznan Mokhtar, Mohammad Effendy Ya’acob, Hasanah Mohd Ghazali, Lee Sin Chang, And Yanty Noorzianna Manaf. 2020. “Coconut (Cocos Nucifera L.) Sap As A Potential Source Of Sugar: Antioxidant And Nutritional Properties.” Food Science & Nutrition 8 (4): 1777–87. Https://Doi.Org/10.1002/Fsn3.1191.

[2] Avinash, Bhagyalakshmi, B. S. Avinash, B. M. Shivalinga, S. Jyothikiran, And M. N. Padmini. 2013. “Going Green With Eco-Friendly Dentistry.” The Journal Of Contemporary Dental Practice 14 (4): 766–69. Https://Doi.Org/10.5005/Jp-Journals-10024-1400.

[3] Chauhan, Durgesh Nandini, Prabhu Raj Singh, Kamal Shah, And Nagendra Singh Chauhan. 2020. Natural Oral Care In Dental Therapy. John Wiley & Sons. Https://Play.Google.Com/Store/Books/Details?Id=V8ppdwaaqbaj.

[4] Chen, Xiuqin, Eric Banan-Mwine Daliri, Namhyeon Kim, Jong-Rae Kim, Daesang Yoo, And Deog-Hwan Oh. 2020. “Microbial Etiology And Prevention Of Dental Caries: Exploiting Natural Products To Inhibit Cariogenic Biofilms.” Pathogens 9 (7). Https://Doi.Org/10.3390/Pathogens9070569.

[5] G, Priyadharshini, Deepak Pandiar, Rajeshkumar Shanmugam, And Reshma Poothakulath Krishnan. 2023. “An In Vitro Evaluation Of Anti-Inflammatory And Antioxidant Activities Of Cocos Nucifera And Triticum Aestivum Formulation.” Cureus 15 (11): E48649. Https://Doi.Org/10.7759/Cureus.48649.

[6] Henrietta, H., K. Kalaiyarasi, And A. Raj. 2022. “Coconut Tree (Cocos Nucifera) Products: A Review Of Global Cultivation And Its Benefits.” Journal Of Sustainability And Environmental Management, May. Https://Doi.Org/10.3126/Josem.V1i2.45377.

[7] Jenner, F., V. Jaleel, R. Kulshrestha, G. Maheswar, P. Rao, And J. Kranthi. 2013. “Evaluating The Antimicrobial Activity Of Commercially Available Herbal Toothpastes On Microorganisms Associated With Diabetes Mellitus.” The Journal Of Contemporary Dental Practice 14 (5): 924–29. Https://Doi.Org/10.5005/JP-JOURNALS-10024-1427.

[8] Kumar, Rajeev, Mohd A. Mirza, Punnoth Poonkuzhi Naseef, Mohamed Saheer Kuruniyan, Foziyah Zakir, And Geeta Aggarwal. 2022. “Exploring The Potential Of Natural Product-Based Nanomedicine For Maintaining Oral Health.” Molecules 27 (5). Https://Doi.Org/10.3390/Molecules27051725.

[9] Lopes, Pedro C., Teresa Carvalho, Ana T. P. C. Gomes, Nelio Veiga, Letícia Blanco, Maria José Correia, And Anna Carolina Volpi Mello-Moura. 2024. “White Spot Lesions: Diagnosis And Treatment - A Systematic Review.” BMC Oral Health 24 (1): 58. Https://Doi.Org/10.1186/S12903-023-03720-6.

[10] Lopez, Maria Asuncion A., Generose B. Solas, And Alicia I. Yu. N.D. “The Antiviral Properties Of A Miracle Oil (Virgin Coconut Oil) From Cocos Nucifera L.: A Review.” Accessed March 19, 2024. Http://Www.Pharmacognosyasia.Com/Files/Other/AJPV5I4p0519.Pdf.

[11] Malik, Shiza, Khalid Muhammad, And Yasir Waheed. 2023. “Nanotechnology: A Revolution In Modern Industry.” Molecules 28 (2). Https://Doi.Org/10.3390/Molecules28020661.

[12] Nimbulkar, Gargi, Vikram Garacha, Vittaldas Shetty, Ketaki Bhor, Kumar Chandan Srivastava, Deepti Shrivastava, And Mohammed G. Sghaireen. 2020. “Microbiological And Clinical Evaluation Of Neem Gel And Chlorhexidine Gel On Dental Plaque And Gingivitis In 20-30 Years Old Adults: A Randomized Parallel-Armed, Double-Blinded Controlled Trial.” Journal Of Pharmacy & Bioallied Sciences 12 (Suppl 1): S345–51. Https://Doi.Org/10.4103/Jpbs.JPBS_101_20.

[13] Prasathkumar, Murugan, And Subramaniam Sadhasivam. 2021. “Chitosan/Hyaluronic Acid/Alginate And An Assorted Polymers Loaded With Honey, Plant, And Marine Compounds For Progressive Wound Healing—Know-How.” International Journal Of Biological Macromolecules 186 (September): 656–85. Https://Doi.Org/10.1016/J.Ijbiomac.2021.07.067.

[14] Qiu, Wei, Yujie Zhou, Zixin Li, Tu Huang, Yuhan Xiao, Lei Cheng, Xian Peng, Lixin Zhang, And Biao Ren. 2020. “Application Of Antibiotics/Antimicrobial Agents On Dental Caries.” Biomed Research International 2020 (January): 5658212. Https://Doi.Org/10.1155/2020/5658212.

[15] Rahman, Farjana, Md Abdul Majed Patwary, Md Abu Bakar Siddique, Muhammad Shahriar Bashar, Md Aminul Haque, Beauty Akter, Rimi Rashid, Md Anamul Haque, And A. K. M. Royhan Uddin. 2022. “Green Synthesis Of Zinc Oxide Nanoparticles Using Cocos Nucifera Leaf Extract: Characterization, Antimicrobial, Antioxidant And Photocatalytic Activity.” Royal Society Open Science 9 (11): 220858. Https://Doi.Org/10.1098/Rsos.220858.

[16] Rajendran, Ratheesh, M. Sadique Hussain, Raghu Sandhya, Mohammed Ashik, Arun Jacob Thomas, And Reni Elizabeth Mammen. 2022. “Effect Of Remineralization Agents On White Spot Lesions: A Systematic Review.” Journal Of Pharmacy & Bioallied Sciences 14 (Suppl 1): S7–12. Https://Doi.Org/10.4103/Jpbs.Jpbs_836_21.

[17] Rukmini, J. N., Sunkari Manasa, Chenna Rohini, Lavanya Putchla Sireesha, Sachan Ritu, And G. K. Umashankar. 2017. “Antibacterial Efficacy Of Tender Coconut Water (Cocos Nucifera L) On Streptococcus Mutans: An In-Vitro Study.” Journal Of International Society Of Preventive & Community Dentistry 7 (2): 130–34. Https://Doi.Org/10.4103/Jispcd.JISPCD_275_16.

[18] Sampson, Victoria, And Ariane Sampson. 2020. “Diagnosis And Treatment Options For Anterior White Spot Lesions.” British Dental Journal 229 (6): 348–52. Https://Doi.Org/10.1038/S41415-020-2057-X.

[19] Uddin, A. K. M. Royhan, Md Abu Bakar Siddique, Farjana Rahman, A. K. M. Atique Ullah, And Rahat Khan. 2020. “Cocos Nucifera Leaf Extract Mediated Green Synthesis Of Silver Nanoparticles For Enhanced Antibacterial Activity.” Journal Of Inorganic And Organometallic Polymers And Materials 30 (9): 3305–16. Https://Doi.Org/10.1007/S10904-020-01506-9.

[20] “Visual Assessment Of Extent Of White Spot Lesions In Subjects Treated With Fixed Orthodontic Appliances: A Retrospective Study.” N.D. Https://Www.Wjoud.Com/Doi/WJOUD/Pdf/10.5005/Jp-Journals-10015-2042.

[21] Balakrishnan N, Subramanian A, Eeswaramoorthy R, Et Al. (September 05, 2023) Enamel Remineralization Efficacy Of Coconut Milk And Lyophilized Coconut Extract In Different Concentrations On Demineralized Enamel Surfaces: An In-Vitro Study. Cureus 15(9): E44712. DOI 10.7759/Cureus.44712

[22] Vogel, Christine Érika, Louise Crovesy, Eliane Lopes Rosado, And Márcia Soares-Mota. 2020. “Effect Of Coconut Oil On Weight Loss And Metabolic Parameters In Men With Obesity: A Randomized Controlled Clinical Trial.” Food & Function 11 (7): 6588–94. Https://Doi.Org/10.1039/D0fo00872a.

[23] “White Spot Lesions: A Serious But Often Ignored Complication Of Orthodontic Treatment.” N.D. Https://Opendentistryjournal.Com/VOLUME/16/ELOCATOR/E187421062202230/FULLTEXT/.

[24] Rieshy V, Priya J, Arivarasu L, Kumar Sr, Devi G. Enhanced Antimicrobial Activity Of Herbal Formulation Mediated Copper Nanoparticles Against Clinical Pathogens. Pcbmb [Internet]. 2020 Nov. 14 [Cited 2024 Apr. 27];21(53-54):52-6.

[25] Maliael, Mathew T.; Varghese, Remmiya M.; Subramanian, Aravind K.. Antibacterial Activity Of Nanoparticle-Coated Orthodontic Archwires: A Systematic Review. Journal Of International Oral Health 15(1):P 1-7, Jan–Feb 2023. | DOI: 10.4103/Jioh.Jioh_152_22

[26] Yong, Jean W. H., Liya Ge, Yan Fei Ng, And Swee Ngin Tan. 2009. “The Chemical Composition And Biological Properties Of Coconut (Cocos Nucifera L.) Water.” Molecules 14 (12): 5144–64. Https://Doi.Org/10.3390/Molecules14125144.

[27] Iffat Nasim, S. Rajeshkumar, V Vishnupriya. Green Synthesis Of Reduced Graphene Oxide Nanoparticles, Its Characterization And Antimicrobial Properties Against Common Oral Pathogens. Int J Dentistry Oral Sci. 2021;8(2):1520-1525. Doi: Dx.Doi.Org/10.19070/2377-8075-21000332

[28] Nasim I, Jabin Z, Kumar SR, Vishnupriya V. Green Synthesis Of Calcium Hydroxide-Coated Silver Nanoparticles Using Andrographis Paniculata And Ocimum Sanctum Linn. Leaf Extracts: An Antimicrobial And Cytotoxic Activity Journal Of Conservative Dentistry : JCD. 2022 Jul-Aug;25(4):369-374. DOI: 10.4103/Jcd.Jcd_411_21. PMID: 36187870; PMCID: PMC9520649.