Evaluation of Biological Response Elicited by Two Novel Tooth Cream Formulations of Cocos nucifera- Cell Line Studies and MTT Assay on Human Gingival Fibroblast

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DOI: 10.21522/TIJPH.2013.SE.24.01.Art026

Authors : Aravind Kumar Subramanian, Nivethigaa Balakrishnan


The current research endeavors to assess the biological reactions induced by two newly formulated tooth creams from Cocos nucifera. Freshly harvested coconut from the Tirupur district of Tamil Nadu underwent processing to create two distinct formulations: one utilizing coconut milk and the other lyophilized coconut extract. Tooth cream samples were prepared and tested against commercial tooth cream following ISO 10993-5 recommendations. Human gingival fibroblast cells were isolated and cultured according to approved protocols, and cytotoxicity evaluations were conducted through MTT assay and live/dead staining. Results indicated high cell viability in both coconut-based formulations, comparable to the commercial tooth cream. Live/dead staining revealed predominantly live cells with minimal cytotoxic effects. Novel coconut-derived tooth creams exhibit comparable biocompatibility to commercial formulations, showing high cell viability and minimal cytotoxicity with human gingival fibroblasts. This suggests coconut-based tooth creams as safe alternatives for oral care, advocating further investigation into their efficacy and tissue safety. Overall, our findings endorse natural compound utilization in dental care formulations.


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